Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29700 and BPHYT_RS29705 are separated by 209 nucleotides BPHYT_RS29705 and BPHYT_RS29710 are separated by 12 nucleotides BPHYT_RS29710 and BPHYT_RS29715 overlap by 4 nucleotides
BPHYT_RS29700: BPHYT_RS29700 - cytochrome C1, at 2,213,904 to 2,215,961
_RS29700
BPHYT_RS29705: BPHYT_RS29705 - (2Fe-2S)-binding protein, at 2,216,171 to 2,216,896
_RS29705
BPHYT_RS29710: BPHYT_RS29710 - FAD-binding molybdopterin dehydrogenase, at 2,216,909 to 2,217,910
_RS29710
BPHYT_RS29715: BPHYT_RS29715 - acylaldehyde oxidase, at 2,217,907 to 2,220,129
_RS29715
Position (kb)
2216
2217
2218 Strain fitness (log2 ratio)
-2
-1
0
1 at 2215.942 kb on - strand at 2216.050 kb on + strand at 2216.050 kb on + strand at 2216.434 kb on - strand, within BPHYT_RS29705 at 2216.442 kb on - strand, within BPHYT_RS29705 at 2216.660 kb on - strand, within BPHYT_RS29705 at 2216.660 kb on - strand, within BPHYT_RS29705 at 2217.593 kb on + strand, within BPHYT_RS29710 at 2217.621 kb on - strand, within BPHYT_RS29710 at 2218.429 kb on + strand, within BPHYT_RS29715 at 2218.874 kb on - strand, within BPHYT_RS29715
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 2,215,942 - -0.2 2,216,050 + +0.1 2,216,050 + +0.9 2,216,434 - BPHYT_RS29705 0.36 -0.1 2,216,442 - BPHYT_RS29705 0.37 -0.1 2,216,660 - BPHYT_RS29705 0.67 +0.1 2,216,660 - BPHYT_RS29705 0.67 +0.1 2,217,593 + BPHYT_RS29710 0.68 +1.1 2,217,621 - BPHYT_RS29710 0.71 +0.3 2,218,429 + BPHYT_RS29715 0.23 -2.1 2,218,874 - BPHYT_RS29715 0.43 -0.9
Or see this region's nucleotide sequence