Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15680 and BPHYT_RS15685 overlap by 1 nucleotides BPHYT_RS15685 and BPHYT_RS15690 are separated by 111 nucleotides BPHYT_RS15690 and BPHYT_RS15695 are separated by 75 nucleotides
BPHYT_RS15680: BPHYT_RS15680 - isoleucine--tRNA ligase, at 3,555,664 to 3,558,501
_RS15680
BPHYT_RS15685: BPHYT_RS15685 - lipoprotein signal peptidase, at 3,558,501 to 3,559,019
_RS15685
BPHYT_RS15690: BPHYT_RS15690 - phosphopantothenate synthase, at 3,559,131 to 3,560,345
_RS15690
BPHYT_RS15695: BPHYT_RS15695 - luciferase, at 3,560,421 to 3,561,440
_RS15695
Position (kb)
3559
3560
3561 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3558.499 kb on + strand at 3559.058 kb on + strand at 3559.058 kb on + strand at 3560.415 kb on - strand at 3560.664 kb on + strand, within BPHYT_RS15695 at 3560.665 kb on - strand, within BPHYT_RS15695 at 3560.693 kb on + strand, within BPHYT_RS15695 at 3560.693 kb on + strand, within BPHYT_RS15695 at 3560.694 kb on - strand, within BPHYT_RS15695 at 3560.794 kb on - strand, within BPHYT_RS15695 at 3560.794 kb on - strand, within BPHYT_RS15695 at 3560.845 kb on + strand, within BPHYT_RS15695 at 3561.075 kb on + strand, within BPHYT_RS15695
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 3,558,499 + +3.7 3,559,058 + -0.0 3,559,058 + +0.4 3,560,415 - -0.5 3,560,664 + BPHYT_RS15695 0.24 +1.3 3,560,665 - BPHYT_RS15695 0.24 -0.7 3,560,693 + BPHYT_RS15695 0.27 +1.2 3,560,693 + BPHYT_RS15695 0.27 -1.7 3,560,694 - BPHYT_RS15695 0.27 +1.2 3,560,794 - BPHYT_RS15695 0.37 +0.2 3,560,794 - BPHYT_RS15695 0.37 -0.4 3,560,845 + BPHYT_RS15695 0.42 -0.1 3,561,075 + BPHYT_RS15695 0.64 -0.1
Or see this region's nucleotide sequence