Experiment: MoYLS4 with Dimethyl Sulfoxide 10 %
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0107 and DvMF_0108 overlap by 4 nucleotides DvMF_0108 and DvMF_R0008 are separated by 325 nucleotides DvMF_R0008 and DvMF_0109 are separated by 80 nucleotides
DvMF_0107: DvMF_0107 - selenide, water dikinase (RefSeq), at 115,303 to 116,355
_0107
DvMF_0108: DvMF_0108 - transcriptional regulator, LysR family (RefSeq), at 116,352 to 117,314
_0108
DvMF_R0008: DvMF_R0008 - tRNA-Leu (RefSeq), at 117,640 to 117,724
_R0008
DvMF_0109: DvMF_0109 - trigger factor (RefSeq), at 117,805 to 119,103
_0109
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-1
0
1 at 115.611 kb on - strand, within DvMF_0107 at 115.622 kb on - strand, within DvMF_0107 at 115.752 kb on + strand, within DvMF_0107 at 116.228 kb on + strand, within DvMF_0107 at 116.237 kb on - strand, within DvMF_0107 at 116.290 kb on + strand at 116.321 kb on + strand at 116.428 kb on - strand at 116.462 kb on - strand, within DvMF_0108 at 116.624 kb on - strand, within DvMF_0108 at 116.929 kb on - strand, within DvMF_0108 at 116.964 kb on + strand, within DvMF_0108 at 117.101 kb on + strand, within DvMF_0108 at 117.101 kb on + strand, within DvMF_0108 at 117.144 kb on - strand, within DvMF_0108 at 117.242 kb on - strand at 117.410 kb on + strand at 117.508 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Dimethyl Sulfoxide 10 % remove 115,611 - DvMF_0107 0.29 -0.5 115,622 - DvMF_0107 0.30 -0.3 115,752 + DvMF_0107 0.43 +0.4 116,228 + DvMF_0107 0.88 -0.6 116,237 - DvMF_0107 0.89 -0.1 116,290 + -0.2 116,321 + +0.0 116,428 - -0.6 116,462 - DvMF_0108 0.11 -0.3 116,624 - DvMF_0108 0.28 -1.1 116,929 - DvMF_0108 0.60 -0.8 116,964 + DvMF_0108 0.64 -0.5 117,101 + DvMF_0108 0.78 -1.3 117,101 + DvMF_0108 0.78 -0.4 117,144 - DvMF_0108 0.82 -0.8 117,242 - -0.5 117,410 + -0.5 117,508 - -1.5
Or see this region's nucleotide sequence