Experiment: Chloramphenicol 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_55 and Pf6N2E2_56 are separated by 45 nucleotides Pf6N2E2_56 and Pf6N2E2_57 overlap by 43 nucleotides Pf6N2E2_57 and Pf6N2E2_58 overlap by 6 nucleotides
Pf6N2E2_55 - Probable hydrolase, at 95,444 to 96,277
_55
Pf6N2E2_56 - FIG002571: 4-hydroxybenzoyl-CoA thioesterase domain protein, at 96,323 to 96,772
_56
Pf6N2E2_57 - hypothetical protein, at 96,730 to 96,891
_57
Pf6N2E2_58 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 96,886 to 97,845
_58
Position (kb) 96
97 Strain fitness (log2 ratio) -1
0
1
2 at 96.352 kb on + strand at 96.367 kb on - strand at 96.411 kb on + strand, within Pf6N2E2_56 at 96.412 kb on - strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.413 kb on + strand, within Pf6N2E2_56 at 96.414 kb on - strand, within Pf6N2E2_56 at 96.414 kb on - strand at 96.414 kb on - strand, within Pf6N2E2_56 at 96.414 kb on - strand, within Pf6N2E2_56 at 96.414 kb on - strand, within Pf6N2E2_56 at 96.543 kb on - strand, within Pf6N2E2_56 at 96.543 kb on - strand, within Pf6N2E2_56 at 96.706 kb on + strand, within Pf6N2E2_56 at 96.707 kb on - strand, within Pf6N2E2_56 at 96.707 kb on - strand, within Pf6N2E2_56 at 96.707 kb on - strand, within Pf6N2E2_56 at 97.377 kb on + strand, within Pf6N2E2_58 at 97.411 kb on + strand, within Pf6N2E2_58 at 97.411 kb on + strand, within Pf6N2E2_58 at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.002 mg/ml remove 96,352 + +0.1 96,367 - +0.1 96,411 + Pf6N2E2_56 0.20 +0.2 96,412 - Pf6N2E2_56 0.20 +0.7 96,413 + Pf6N2E2_56 0.20 -0.4 96,413 + Pf6N2E2_56 0.20 -0.8 96,413 + Pf6N2E2_56 0.20 -0.5 96,413 + Pf6N2E2_56 0.20 -0.3 96,413 + Pf6N2E2_56 0.20 +0.2 96,413 + Pf6N2E2_56 0.20 +0.3 96,413 + Pf6N2E2_56 0.20 -0.9 96,413 + Pf6N2E2_56 0.20 -0.3 96,414 - Pf6N2E2_56 0.20 -0.1 96,414 - +1.9 96,414 - Pf6N2E2_56 0.20 -0.0 96,414 - Pf6N2E2_56 0.20 -1.1 96,414 - Pf6N2E2_56 0.20 -0.7 96,543 - Pf6N2E2_56 0.49 +0.0 96,543 - Pf6N2E2_56 0.49 +1.0 96,706 + Pf6N2E2_56 0.85 +0.4 96,707 - Pf6N2E2_56 0.85 +0.5 96,707 - Pf6N2E2_56 0.85 +0.4 96,707 - Pf6N2E2_56 0.85 +0.0 97,377 + Pf6N2E2_58 0.51 -0.5 97,411 + Pf6N2E2_58 0.55 -0.9 97,411 + Pf6N2E2_58 0.55 -0.2 97,889 + +0.2 97,889 + +0.3 97,889 + +0.4 97,889 + +0.1 97,889 + +0.5 97,889 + +0.4 97,889 + +0.5
Or see this region's nucleotide sequence