Experiment: Chloramphenicol 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2580 and Pf6N2E2_2581 overlap by 1 nucleotides Pf6N2E2_2581 and Pf6N2E2_2582 are separated by 0 nucleotides Pf6N2E2_2582 and Pf6N2E2_2583 are separated by 72 nucleotides Pf6N2E2_2583 and Pf6N2E2_2584 are separated by 9 nucleotides Pf6N2E2_2584 and Pf6N2E2_2585 are separated by 232 nucleotides
Pf6N2E2_2580 - Flagellar biosynthesis protein FliP, at 3,038,941 to 3,039,690
_2580
Pf6N2E2_2581 - Flagellar biosynthesis protein FliQ, at 3,039,690 to 3,040,127
_2581
Pf6N2E2_2582 - Flagellar motor switch protein FliN, at 3,040,128 to 3,040,586
_2582
Pf6N2E2_2583 - Flagellar motor switch protein FliM, at 3,040,659 to 3,041,627
_2583
Pf6N2E2_2584 - Flagellar biosynthesis protein FliL, at 3,041,637 to 3,042,140
_2584
Pf6N2E2_2585 - Flagellar hook-length control protein FliK, at 3,042,373 to 3,043,746
_2585
Position (kb)
3040
3041
3042 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3039.692 kb on - strand at 3039.692 kb on - strand at 3039.855 kb on + strand, within Pf6N2E2_2581 at 3040.242 kb on + strand, within Pf6N2E2_2582 at 3040.243 kb on - strand, within Pf6N2E2_2582 at 3040.243 kb on - strand, within Pf6N2E2_2582 at 3040.243 kb on - strand, within Pf6N2E2_2582 at 3040.617 kb on - strand at 3040.617 kb on - strand at 3040.641 kb on + strand at 3040.641 kb on + strand at 3040.641 kb on + strand at 3040.972 kb on + strand, within Pf6N2E2_2583 at 3040.973 kb on - strand, within Pf6N2E2_2583 at 3041.095 kb on + strand, within Pf6N2E2_2583 at 3041.096 kb on - strand, within Pf6N2E2_2583 at 3041.338 kb on + strand, within Pf6N2E2_2583 at 3041.339 kb on - strand, within Pf6N2E2_2583 at 3041.353 kb on + strand, within Pf6N2E2_2583 at 3041.353 kb on + strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.354 kb on - strand, within Pf6N2E2_2583 at 3041.515 kb on + strand, within Pf6N2E2_2583 at 3041.639 kb on - strand at 3041.639 kb on - strand at 3041.647 kb on - strand at 3041.870 kb on + strand, within Pf6N2E2_2584 at 3041.887 kb on + strand, within Pf6N2E2_2584 at 3041.887 kb on + strand, within Pf6N2E2_2584 at 3041.887 kb on + strand, within Pf6N2E2_2584 at 3041.887 kb on + strand, within Pf6N2E2_2584 at 3041.888 kb on - strand, within Pf6N2E2_2584 at 3041.888 kb on - strand, within Pf6N2E2_2584 at 3041.888 kb on - strand, within Pf6N2E2_2584 at 3041.888 kb on - strand, within Pf6N2E2_2584 at 3041.911 kb on + strand, within Pf6N2E2_2584 at 3041.911 kb on + strand, within Pf6N2E2_2584 at 3041.911 kb on + strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3041.912 kb on - strand, within Pf6N2E2_2584 at 3042.019 kb on + strand, within Pf6N2E2_2584 at 3042.019 kb on + strand, within Pf6N2E2_2584 at 3042.040 kb on - strand, within Pf6N2E2_2584 at 3042.040 kb on - strand, within Pf6N2E2_2584 at 3042.040 kb on - strand, within Pf6N2E2_2584 at 3042.114 kb on + strand at 3042.114 kb on + strand at 3042.146 kb on + strand at 3042.147 kb on - strand at 3042.157 kb on + strand at 3042.201 kb on + strand at 3042.304 kb on + strand at 3042.305 kb on - strand at 3042.315 kb on + strand at 3042.315 kb on + strand at 3042.315 kb on + strand at 3042.315 kb on + strand at 3042.380 kb on + strand at 3042.380 kb on + strand at 3042.380 kb on + strand at 3042.381 kb on - strand at 3042.381 kb on - strand at 3042.383 kb on + strand at 3042.384 kb on - strand at 3042.417 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloramphenicol 0.002 mg/ml remove 3,039,692 - +0.6 3,039,692 - -0.9 3,039,855 + Pf6N2E2_2581 0.38 -0.0 3,040,242 + Pf6N2E2_2582 0.25 +0.7 3,040,243 - Pf6N2E2_2582 0.25 +0.3 3,040,243 - Pf6N2E2_2582 0.25 +1.1 3,040,243 - Pf6N2E2_2582 0.25 +0.7 3,040,617 - +0.6 3,040,617 - -0.1 3,040,641 + +0.6 3,040,641 + +0.6 3,040,641 + -0.1 3,040,972 + Pf6N2E2_2583 0.32 +2.2 3,040,973 - Pf6N2E2_2583 0.32 +0.8 3,041,095 + Pf6N2E2_2583 0.45 +0.6 3,041,096 - Pf6N2E2_2583 0.45 +0.4 3,041,338 + Pf6N2E2_2583 0.70 +0.4 3,041,339 - Pf6N2E2_2583 0.70 +0.4 3,041,353 + Pf6N2E2_2583 0.72 +0.4 3,041,353 + Pf6N2E2_2583 0.72 +0.2 3,041,354 - Pf6N2E2_2583 0.72 +0.7 3,041,354 - Pf6N2E2_2583 0.72 -0.5 3,041,354 - Pf6N2E2_2583 0.72 +0.3 3,041,354 - Pf6N2E2_2583 0.72 +0.4 3,041,354 - Pf6N2E2_2583 0.72 +0.2 3,041,515 + Pf6N2E2_2583 0.88 +0.8 3,041,639 - +0.1 3,041,639 - -0.7 3,041,647 - -0.5 3,041,870 + Pf6N2E2_2584 0.46 +0.0 3,041,887 + Pf6N2E2_2584 0.50 +0.6 3,041,887 + Pf6N2E2_2584 0.50 +1.7 3,041,887 + Pf6N2E2_2584 0.50 -0.1 3,041,887 + Pf6N2E2_2584 0.50 +0.2 3,041,888 - Pf6N2E2_2584 0.50 -0.2 3,041,888 - Pf6N2E2_2584 0.50 +0.9 3,041,888 - Pf6N2E2_2584 0.50 -0.4 3,041,888 - Pf6N2E2_2584 0.50 +0.1 3,041,911 + Pf6N2E2_2584 0.54 +1.5 3,041,911 + Pf6N2E2_2584 0.54 +0.1 3,041,911 + Pf6N2E2_2584 0.54 +0.5 3,041,912 - Pf6N2E2_2584 0.55 +1.1 3,041,912 - Pf6N2E2_2584 0.55 +0.0 3,041,912 - Pf6N2E2_2584 0.55 +0.4 3,041,912 - Pf6N2E2_2584 0.55 -0.3 3,041,912 - Pf6N2E2_2584 0.55 +0.3 3,041,912 - Pf6N2E2_2584 0.55 +0.1 3,042,019 + Pf6N2E2_2584 0.76 -0.1 3,042,019 + Pf6N2E2_2584 0.76 -0.0 3,042,040 - Pf6N2E2_2584 0.80 -0.2 3,042,040 - Pf6N2E2_2584 0.80 +0.7 3,042,040 - Pf6N2E2_2584 0.80 +0.3 3,042,114 + -0.0 3,042,114 + -0.1 3,042,146 + +0.0 3,042,147 - +1.3 3,042,157 + +0.2 3,042,201 + +0.2 3,042,304 + +0.6 3,042,305 - -0.7 3,042,315 + -0.6 3,042,315 + -1.9 3,042,315 + +0.6 3,042,315 + +0.0 3,042,380 + +0.0 3,042,380 + +0.8 3,042,380 + +0.5 3,042,381 - +0.4 3,042,381 - +0.1 3,042,383 + +0.6 3,042,384 - +0.5 3,042,417 + +0.3
Or see this region's nucleotide sequence