Experiment: Recovery broth with Tiamulin 1e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14490 and BBR_RS14495 are separated by 54 nucleotides BBR_RS14495 and BBR_RS14500 are separated by 183 nucleotides
BBR_RS14490: BBR_RS14490 - ribonuclease D, at 1,025,170 to 1,026,471
_RS14490
BBR_RS14495: BBR_RS14495 - trigger factor, at 1,026,526 to 1,027,899
_RS14495
BBR_RS14500: BBR_RS14500 - hypothetical protein, at 1,028,083 to 1,029,483
_RS14500
Position (kb)
1026
1027
1028 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1025.667 kb on - strand, within BBR_RS14490 at 1025.681 kb on - strand, within BBR_RS14490 at 1025.683 kb on - strand, within BBR_RS14490 at 1025.698 kb on - strand, within BBR_RS14490 at 1025.726 kb on + strand, within BBR_RS14490 at 1025.739 kb on - strand, within BBR_RS14490 at 1025.777 kb on + strand, within BBR_RS14490 at 1025.785 kb on - strand, within BBR_RS14490 at 1025.787 kb on + strand, within BBR_RS14490 at 1025.787 kb on + strand, within BBR_RS14490 at 1025.851 kb on + strand, within BBR_RS14490 at 1025.883 kb on + strand, within BBR_RS14490 at 1025.915 kb on - strand, within BBR_RS14490 at 1025.944 kb on - strand, within BBR_RS14490 at 1025.949 kb on - strand, within BBR_RS14490 at 1025.952 kb on - strand, within BBR_RS14490 at 1025.960 kb on - strand, within BBR_RS14490 at 1025.960 kb on - strand, within BBR_RS14490 at 1026.021 kb on - strand, within BBR_RS14490 at 1026.031 kb on - strand, within BBR_RS14490 at 1026.035 kb on - strand, within BBR_RS14490 at 1026.037 kb on - strand, within BBR_RS14490 at 1026.056 kb on - strand, within BBR_RS14490 at 1026.133 kb on + strand, within BBR_RS14490 at 1026.133 kb on + strand, within BBR_RS14490 at 1026.146 kb on - strand, within BBR_RS14490 at 1026.155 kb on + strand, within BBR_RS14490 at 1026.212 kb on - strand, within BBR_RS14490 at 1026.261 kb on - strand, within BBR_RS14490 at 1026.265 kb on + strand, within BBR_RS14490 at 1026.279 kb on - strand, within BBR_RS14490 at 1026.517 kb on + strand at 1026.639 kb on + strand at 1026.760 kb on + strand, within BBR_RS14495 at 1027.204 kb on + strand, within BBR_RS14495 at 1027.217 kb on + strand, within BBR_RS14495 at 1027.257 kb on + strand, within BBR_RS14495 at 1027.304 kb on + strand, within BBR_RS14495 at 1027.425 kb on + strand, within BBR_RS14495 at 1027.461 kb on + strand, within BBR_RS14495 at 1027.689 kb on + strand, within BBR_RS14495 at 1027.719 kb on + strand, within BBR_RS14495 at 1027.855 kb on + strand at 1027.882 kb on + strand at 1028.003 kb on + strand at 1028.003 kb on + strand at 1028.003 kb on + strand at 1028.024 kb on - strand at 1028.041 kb on + strand at 1028.069 kb on + strand at 1028.069 kb on + strand at 1028.085 kb on - strand at 1028.085 kb on - strand at 1028.132 kb on + strand at 1028.146 kb on - strand at 1028.184 kb on + strand at 1028.184 kb on + strand at 1028.192 kb on - strand at 1028.226 kb on - strand, within BBR_RS14500 at 1028.243 kb on + strand, within BBR_RS14500 at 1028.243 kb on - strand, within BBR_RS14500 at 1028.432 kb on - strand, within BBR_RS14500 at 1028.464 kb on + strand, within BBR_RS14500 at 1028.528 kb on + strand, within BBR_RS14500 at 1028.552 kb on - strand, within BBR_RS14500 at 1028.642 kb on + strand, within BBR_RS14500 at 1028.643 kb on - strand, within BBR_RS14500 at 1028.671 kb on - strand, within BBR_RS14500 at 1028.711 kb on - strand, within BBR_RS14500 at 1028.730 kb on - strand, within BBR_RS14500 at 1028.756 kb on + strand, within BBR_RS14500 at 1028.756 kb on + strand, within BBR_RS14500 at 1028.779 kb on + strand, within BBR_RS14500 at 1028.782 kb on - strand, within BBR_RS14500 at 1028.784 kb on + strand, within BBR_RS14500 at 1028.792 kb on - strand, within BBR_RS14500 at 1028.792 kb on - strand, within BBR_RS14500 at 1028.792 kb on - strand, within BBR_RS14500 at 1028.827 kb on + strand, within BBR_RS14500 at 1028.835 kb on - strand, within BBR_RS14500 at 1028.839 kb on + strand, within BBR_RS14500 at 1028.893 kb on + strand, within BBR_RS14500
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Tiamulin 1e-04 mM remove 1,025,667 - BBR_RS14490 0.38 +1.3 1,025,681 - BBR_RS14490 0.39 +0.2 1,025,683 - BBR_RS14490 0.39 -0.7 1,025,698 - BBR_RS14490 0.41 +0.1 1,025,726 + BBR_RS14490 0.43 +0.2 1,025,739 - BBR_RS14490 0.44 +0.0 1,025,777 + BBR_RS14490 0.47 +0.8 1,025,785 - BBR_RS14490 0.47 -0.4 1,025,787 + BBR_RS14490 0.47 +0.7 1,025,787 + BBR_RS14490 0.47 -0.0 1,025,851 + BBR_RS14490 0.52 +0.4 1,025,883 + BBR_RS14490 0.55 -1.0 1,025,915 - BBR_RS14490 0.57 +0.1 1,025,944 - BBR_RS14490 0.59 +0.7 1,025,949 - BBR_RS14490 0.60 -1.2 1,025,952 - BBR_RS14490 0.60 +0.8 1,025,960 - BBR_RS14490 0.61 +0.9 1,025,960 - BBR_RS14490 0.61 -0.0 1,026,021 - BBR_RS14490 0.65 -0.0 1,026,031 - BBR_RS14490 0.66 -1.0 1,026,035 - BBR_RS14490 0.66 -0.1 1,026,037 - BBR_RS14490 0.67 +0.4 1,026,056 - BBR_RS14490 0.68 +0.2 1,026,133 + BBR_RS14490 0.74 +0.8 1,026,133 + BBR_RS14490 0.74 -0.5 1,026,146 - BBR_RS14490 0.75 -0.4 1,026,155 + BBR_RS14490 0.76 -1.5 1,026,212 - BBR_RS14490 0.80 -1.0 1,026,261 - BBR_RS14490 0.84 -0.6 1,026,265 + BBR_RS14490 0.84 -0.4 1,026,279 - BBR_RS14490 0.85 -0.1 1,026,517 + +2.4 1,026,639 + +0.4 1,026,760 + BBR_RS14495 0.17 +0.1 1,027,204 + BBR_RS14495 0.49 -1.6 1,027,217 + BBR_RS14495 0.50 -0.1 1,027,257 + BBR_RS14495 0.53 -0.1 1,027,304 + BBR_RS14495 0.57 -1.9 1,027,425 + BBR_RS14495 0.65 +1.7 1,027,461 + BBR_RS14495 0.68 -0.1 1,027,689 + BBR_RS14495 0.85 -0.5 1,027,719 + BBR_RS14495 0.87 -0.5 1,027,855 + -1.0 1,027,882 + -0.8 1,028,003 + +0.6 1,028,003 + +0.2 1,028,003 + -0.6 1,028,024 - +0.3 1,028,041 + -0.1 1,028,069 + +0.8 1,028,069 + -0.1 1,028,085 - +1.1 1,028,085 - +1.2 1,028,132 + +0.2 1,028,146 - -0.8 1,028,184 + +0.4 1,028,184 + +0.1 1,028,192 - -0.2 1,028,226 - BBR_RS14500 0.10 +1.3 1,028,243 + BBR_RS14500 0.11 -1.1 1,028,243 - BBR_RS14500 0.11 -0.3 1,028,432 - BBR_RS14500 0.25 +0.1 1,028,464 + BBR_RS14500 0.27 +0.1 1,028,528 + BBR_RS14500 0.32 -0.0 1,028,552 - BBR_RS14500 0.33 +0.8 1,028,642 + BBR_RS14500 0.40 +0.7 1,028,643 - BBR_RS14500 0.40 +0.0 1,028,671 - BBR_RS14500 0.42 -0.1 1,028,711 - BBR_RS14500 0.45 +0.6 1,028,730 - BBR_RS14500 0.46 +0.6 1,028,756 + BBR_RS14500 0.48 +0.5 1,028,756 + BBR_RS14500 0.48 +0.4 1,028,779 + BBR_RS14500 0.50 +0.3 1,028,782 - BBR_RS14500 0.50 -1.1 1,028,784 + BBR_RS14500 0.50 -2.5 1,028,792 - BBR_RS14500 0.51 -0.5 1,028,792 - BBR_RS14500 0.51 +2.4 1,028,792 - BBR_RS14500 0.51 +1.3 1,028,827 + BBR_RS14500 0.53 -0.7 1,028,835 - BBR_RS14500 0.54 +0.8 1,028,839 + BBR_RS14500 0.54 -0.0 1,028,893 + BBR_RS14500 0.58 +1.0
Or see this region's nucleotide sequence