Experiment: Rifampicin 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4541 and Pf6N2E2_4542 are separated by 206 nucleotides Pf6N2E2_4542 and Pf6N2E2_4543 are separated by 6 nucleotides Pf6N2E2_4543 and Pf6N2E2_4544 overlap by 4 nucleotides Pf6N2E2_4544 and Pf6N2E2_4545 are separated by 25 nucleotides Pf6N2E2_4545 and Pf6N2E2_4546 overlap by 14 nucleotides
Pf6N2E2_4541 - hypothetical protein, at 5,274,006 to 5,274,455
_4541
Pf6N2E2_4542 - negative regulator of hrp expression HrpV, at 5,274,662 to 5,275,039
_4542
Pf6N2E2_4543 - hypothetical protein, at 5,275,046 to 5,275,255
_4543
Pf6N2E2_4544 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 5,275,252 to 5,277,369
_4544
Pf6N2E2_4545 - hypothetical protein, at 5,277,395 to 5,277,832
_4545
Pf6N2E2_4546 - hypothetical protein, at 5,277,819 to 5,278,043
_4546
Position (kb)
5275
5276
5277
5278 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 5274.362 kb on - strand, within Pf6N2E2_4541 at 5274.471 kb on + strand at 5274.471 kb on + strand at 5274.471 kb on + strand at 5274.472 kb on - strand at 5274.666 kb on + strand at 5274.666 kb on + strand at 5274.667 kb on - strand at 5274.678 kb on + strand at 5274.678 kb on + strand at 5274.679 kb on - strand at 5274.806 kb on + strand, within Pf6N2E2_4542 at 5274.806 kb on + strand, within Pf6N2E2_4542 at 5274.807 kb on - strand, within Pf6N2E2_4542 at 5274.807 kb on - strand, within Pf6N2E2_4542 at 5274.822 kb on + strand, within Pf6N2E2_4542 at 5274.893 kb on + strand, within Pf6N2E2_4542 at 5274.893 kb on + strand, within Pf6N2E2_4542 at 5274.893 kb on + strand, within Pf6N2E2_4542 at 5274.894 kb on - strand, within Pf6N2E2_4542 at 5274.894 kb on - strand, within Pf6N2E2_4542 at 5274.899 kb on + strand, within Pf6N2E2_4542 at 5274.957 kb on - strand, within Pf6N2E2_4542 at 5274.957 kb on - strand, within Pf6N2E2_4542 at 5274.957 kb on - strand, within Pf6N2E2_4542 at 5274.967 kb on - strand, within Pf6N2E2_4542 at 5274.967 kb on - strand, within Pf6N2E2_4542 at 5274.967 kb on - strand, within Pf6N2E2_4542 at 5275.017 kb on - strand at 5275.017 kb on - strand at 5275.017 kb on - strand at 5275.017 kb on - strand at 5275.017 kb on - strand at 5275.039 kb on + strand at 5275.039 kb on + strand at 5275.039 kb on + strand at 5275.040 kb on - strand at 5275.040 kb on - strand at 5275.069 kb on + strand, within Pf6N2E2_4543 at 5275.070 kb on - strand, within Pf6N2E2_4543 at 5275.226 kb on + strand, within Pf6N2E2_4543 at 5275.226 kb on + strand, within Pf6N2E2_4543 at 5275.557 kb on - strand, within Pf6N2E2_4544 at 5275.722 kb on - strand, within Pf6N2E2_4544 at 5276.129 kb on + strand, within Pf6N2E2_4544 at 5276.129 kb on + strand, within Pf6N2E2_4544 at 5276.130 kb on - strand, within Pf6N2E2_4544 at 5276.253 kb on - strand, within Pf6N2E2_4544 at 5276.465 kb on + strand, within Pf6N2E2_4544 at 5276.465 kb on + strand, within Pf6N2E2_4544 at 5276.466 kb on - strand, within Pf6N2E2_4544 at 5276.466 kb on - strand, within Pf6N2E2_4544 at 5276.750 kb on + strand, within Pf6N2E2_4544 at 5276.751 kb on - strand, within Pf6N2E2_4544 at 5276.861 kb on + strand, within Pf6N2E2_4544 at 5276.862 kb on - strand, within Pf6N2E2_4544 at 5276.862 kb on - strand, within Pf6N2E2_4544 at 5277.026 kb on + strand, within Pf6N2E2_4544 at 5277.027 kb on - strand, within Pf6N2E2_4544 at 5277.027 kb on - strand, within Pf6N2E2_4544 at 5277.027 kb on - strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.041 kb on + strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.042 kb on - strand, within Pf6N2E2_4544 at 5277.148 kb on + strand, within Pf6N2E2_4544 at 5277.168 kb on + strand at 5277.169 kb on - strand at 5277.187 kb on + strand at 5277.219 kb on - strand at 5277.224 kb on + strand at 5277.224 kb on + strand at 5277.224 kb on + strand at 5277.352 kb on + strand at 5277.352 kb on + strand at 5277.352 kb on + strand at 5277.352 kb on + strand at 5277.352 kb on + strand at 5277.353 kb on - strand at 5277.377 kb on + strand at 5277.478 kb on + strand, within Pf6N2E2_4545 at 5278.003 kb on + strand, within Pf6N2E2_4546 at 5278.003 kb on + strand, within Pf6N2E2_4546 at 5278.003 kb on + strand, within Pf6N2E2_4546 at 5278.003 kb on + strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.004 kb on - strand, within Pf6N2E2_4546 at 5278.068 kb on + strand at 5278.068 kb on + strand at 5278.068 kb on + strand at 5278.090 kb on + strand at 5278.091 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.215 kb on - strand at 5278.219 kb on - strand at 5278.219 kb on - strand at 5278.244 kb on + strand at 5278.244 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.003 mg/ml remove 5,274,362 - Pf6N2E2_4541 0.79 +0.1 5,274,471 + +0.3 5,274,471 + +0.6 5,274,471 + +0.1 5,274,472 - +1.1 5,274,666 + +0.7 5,274,666 + -0.0 5,274,667 - -0.5 5,274,678 + -0.3 5,274,678 + +0.5 5,274,679 - +0.5 5,274,806 + Pf6N2E2_4542 0.38 -0.2 5,274,806 + Pf6N2E2_4542 0.38 -0.8 5,274,807 - Pf6N2E2_4542 0.38 +2.1 5,274,807 - Pf6N2E2_4542 0.38 -0.1 5,274,822 + Pf6N2E2_4542 0.42 -1.2 5,274,893 + Pf6N2E2_4542 0.61 +0.3 5,274,893 + Pf6N2E2_4542 0.61 +0.3 5,274,893 + Pf6N2E2_4542 0.61 -0.8 5,274,894 - Pf6N2E2_4542 0.61 -0.2 5,274,894 - Pf6N2E2_4542 0.61 +0.4 5,274,899 + Pf6N2E2_4542 0.63 -0.7 5,274,957 - Pf6N2E2_4542 0.78 +0.2 5,274,957 - Pf6N2E2_4542 0.78 -1.2 5,274,957 - Pf6N2E2_4542 0.78 +0.1 5,274,967 - Pf6N2E2_4542 0.81 -1.1 5,274,967 - Pf6N2E2_4542 0.81 -0.1 5,274,967 - Pf6N2E2_4542 0.81 +0.3 5,275,017 - -0.2 5,275,017 - +0.4 5,275,017 - +0.8 5,275,017 - -0.3 5,275,017 - -0.5 5,275,039 + +0.2 5,275,039 + +0.3 5,275,039 + -2.8 5,275,040 - -0.3 5,275,040 - -0.6 5,275,069 + Pf6N2E2_4543 0.11 -0.1 5,275,070 - Pf6N2E2_4543 0.11 -0.9 5,275,226 + Pf6N2E2_4543 0.86 -1.6 5,275,226 + Pf6N2E2_4543 0.86 +0.1 5,275,557 - Pf6N2E2_4544 0.14 -0.2 5,275,722 - Pf6N2E2_4544 0.22 -0.6 5,276,129 + Pf6N2E2_4544 0.41 -1.1 5,276,129 + Pf6N2E2_4544 0.41 -0.1 5,276,130 - Pf6N2E2_4544 0.41 -0.7 5,276,253 - Pf6N2E2_4544 0.47 -0.2 5,276,465 + Pf6N2E2_4544 0.57 +0.7 5,276,465 + Pf6N2E2_4544 0.57 -2.0 5,276,466 - Pf6N2E2_4544 0.57 +0.2 5,276,466 - Pf6N2E2_4544 0.57 -0.1 5,276,750 + Pf6N2E2_4544 0.71 -1.0 5,276,751 - Pf6N2E2_4544 0.71 -0.7 5,276,861 + Pf6N2E2_4544 0.76 -0.7 5,276,862 - Pf6N2E2_4544 0.76 -0.4 5,276,862 - Pf6N2E2_4544 0.76 -1.6 5,277,026 + Pf6N2E2_4544 0.84 -0.6 5,277,027 - Pf6N2E2_4544 0.84 -1.0 5,277,027 - Pf6N2E2_4544 0.84 -0.2 5,277,027 - Pf6N2E2_4544 0.84 -1.3 5,277,041 + Pf6N2E2_4544 0.84 -1.7 5,277,041 + Pf6N2E2_4544 0.84 +0.3 5,277,041 + Pf6N2E2_4544 0.84 -0.2 5,277,041 + Pf6N2E2_4544 0.84 -0.9 5,277,041 + Pf6N2E2_4544 0.84 +0.3 5,277,041 + Pf6N2E2_4544 0.84 -0.2 5,277,041 + Pf6N2E2_4544 0.84 +0.5 5,277,042 - Pf6N2E2_4544 0.85 -0.7 5,277,042 - Pf6N2E2_4544 0.85 +0.4 5,277,042 - Pf6N2E2_4544 0.85 -0.3 5,277,042 - Pf6N2E2_4544 0.85 -0.0 5,277,042 - Pf6N2E2_4544 0.85 -1.7 5,277,042 - Pf6N2E2_4544 0.85 +0.9 5,277,148 + Pf6N2E2_4544 0.90 +0.2 5,277,168 + +0.2 5,277,169 - +1.5 5,277,187 + -0.5 5,277,219 - -1.8 5,277,224 + +0.5 5,277,224 + +0.4 5,277,224 + -0.0 5,277,352 + -1.8 5,277,352 + -0.2 5,277,352 + +0.3 5,277,352 + -0.7 5,277,352 + +0.2 5,277,353 - -2.1 5,277,377 + +0.2 5,277,478 + Pf6N2E2_4545 0.19 -0.7 5,278,003 + Pf6N2E2_4546 0.82 -0.9 5,278,003 + Pf6N2E2_4546 0.82 +4.2 5,278,003 + Pf6N2E2_4546 0.82 +0.4 5,278,003 + Pf6N2E2_4546 0.82 +0.2 5,278,004 - Pf6N2E2_4546 0.82 -0.4 5,278,004 - Pf6N2E2_4546 0.82 +1.4 5,278,004 - Pf6N2E2_4546 0.82 -0.1 5,278,004 - Pf6N2E2_4546 0.82 -1.0 5,278,004 - Pf6N2E2_4546 0.82 +0.2 5,278,068 + -0.6 5,278,068 + -0.3 5,278,068 + +0.4 5,278,090 + +0.1 5,278,091 - -0.7 5,278,215 - -0.6 5,278,215 - +1.0 5,278,215 - -0.9 5,278,215 - -0.8 5,278,215 - -0.9 5,278,215 - +0.1 5,278,219 - -0.6 5,278,219 - +0.8 5,278,244 + -0.1 5,278,244 + -0.2
Or see this region's nucleotide sequence