Experiment: Rifampicin 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_327 and Pf6N2E2_328 are separated by 246 nucleotides Pf6N2E2_328 and Pf6N2E2_329 are separated by 161 nucleotides Pf6N2E2_329 and Pf6N2E2_330 are separated by 241 nucleotides
Pf6N2E2_327 - Alginate lyase precursor (EC 4.2.2.3), at 395,293 to 395,949
_327
Pf6N2E2_328 - hypothetical protein, at 396,196 to 396,531
_328
Pf6N2E2_329 - L-lactate permease, at 396,693 to 398,456
_329
Pf6N2E2_330 - Mesenchymal stem cell protein DSCD75, at 398,698 to 399,249
_330
Position (kb) 396
397
398
399 Strain fitness (log2 ratio) -2
-1
0
1
2 at 395.855 kb on + strand, within Pf6N2E2_327 at 395.855 kb on + strand, within Pf6N2E2_327 at 395.856 kb on - strand, within Pf6N2E2_327 at 395.856 kb on - strand, within Pf6N2E2_327 at 395.952 kb on + strand at 395.952 kb on + strand at 395.952 kb on + strand at 395.952 kb on + strand at 396.112 kb on + strand at 396.146 kb on + strand at 396.146 kb on + strand at 396.146 kb on + strand at 396.283 kb on + strand, within Pf6N2E2_328 at 396.283 kb on + strand, within Pf6N2E2_328 at 396.283 kb on + strand, within Pf6N2E2_328 at 396.284 kb on - strand, within Pf6N2E2_328 at 396.298 kb on + strand, within Pf6N2E2_328 at 396.298 kb on + strand, within Pf6N2E2_328 at 396.482 kb on - strand, within Pf6N2E2_328 at 396.547 kb on + strand at 396.547 kb on + strand at 396.547 kb on + strand at 396.548 kb on - strand at 396.548 kb on - strand at 396.548 kb on - strand at 396.548 kb on - strand at 396.548 kb on - strand at 396.565 kb on - strand at 396.574 kb on + strand at 396.574 kb on + strand at 396.713 kb on + strand at 396.714 kb on - strand at 396.714 kb on - strand at 396.714 kb on - strand at 396.714 kb on - strand at 396.714 kb on - strand at 396.714 kb on - strand at 396.714 kb on - strand at 396.733 kb on + strand at 396.733 kb on + strand at 396.734 kb on - strand at 396.734 kb on - strand at 396.734 kb on - strand at 396.734 kb on - strand at 396.734 kb on - strand at 396.734 kb on - strand at 397.126 kb on - strand, within Pf6N2E2_329 at 397.126 kb on - strand, within Pf6N2E2_329 at 397.126 kb on - strand, within Pf6N2E2_329 at 397.134 kb on - strand, within Pf6N2E2_329 at 397.134 kb on - strand, within Pf6N2E2_329 at 397.171 kb on + strand, within Pf6N2E2_329 at 397.172 kb on - strand, within Pf6N2E2_329 at 397.199 kb on - strand, within Pf6N2E2_329 at 397.199 kb on - strand, within Pf6N2E2_329 at 397.274 kb on - strand, within Pf6N2E2_329 at 397.292 kb on + strand, within Pf6N2E2_329 at 397.729 kb on + strand, within Pf6N2E2_329 at 397.729 kb on + strand, within Pf6N2E2_329 at 397.730 kb on - strand, within Pf6N2E2_329 at 398.248 kb on + strand, within Pf6N2E2_329 at 398.249 kb on - strand, within Pf6N2E2_329 at 398.437 kb on + strand at 398.438 kb on - strand at 398.474 kb on + strand at 398.516 kb on + strand at 398.516 kb on + strand at 398.516 kb on + strand at 398.516 kb on + strand at 398.516 kb on + strand at 398.517 kb on - strand at 398.517 kb on - strand at 398.517 kb on - strand at 398.518 kb on + strand at 398.519 kb on - strand at 398.623 kb on + strand at 398.683 kb on + strand at 398.683 kb on + strand at 398.683 kb on + strand at 398.683 kb on + strand at 398.684 kb on - strand at 398.684 kb on - strand at 399.074 kb on + strand, within Pf6N2E2_330 at 399.074 kb on + strand, within Pf6N2E2_330 at 399.075 kb on - strand, within Pf6N2E2_330 at 399.107 kb on + strand, within Pf6N2E2_330 at 399.108 kb on - strand, within Pf6N2E2_330 at 399.108 kb on - strand, within Pf6N2E2_330 at 399.283 kb on + strand at 399.284 kb on - strand at 399.307 kb on - strand at 399.392 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.003 mg/ml remove 395,855 + Pf6N2E2_327 0.86 -1.1 395,855 + Pf6N2E2_327 0.86 -0.4 395,856 - Pf6N2E2_327 0.86 -0.1 395,856 - Pf6N2E2_327 0.86 +0.7 395,952 + +0.4 395,952 + -0.1 395,952 + -2.4 395,952 + +1.3 396,112 + +0.6 396,146 + +1.2 396,146 + -1.0 396,146 + +0.9 396,283 + Pf6N2E2_328 0.26 +0.2 396,283 + Pf6N2E2_328 0.26 +1.2 396,283 + Pf6N2E2_328 0.26 +1.1 396,284 - Pf6N2E2_328 0.26 +1.0 396,298 + Pf6N2E2_328 0.30 -1.0 396,298 + Pf6N2E2_328 0.30 +1.2 396,482 - Pf6N2E2_328 0.85 +0.5 396,547 + +0.3 396,547 + -1.0 396,547 + -0.7 396,548 - +1.4 396,548 - -0.1 396,548 - -0.9 396,548 - -0.0 396,548 - -0.7 396,565 - -1.3 396,574 + -0.5 396,574 + +0.5 396,713 + -0.1 396,714 - +0.4 396,714 - -0.1 396,714 - +0.2 396,714 - -1.1 396,714 - +0.3 396,714 - -0.7 396,714 - +0.3 396,733 + -0.4 396,733 + -1.7 396,734 - +2.1 396,734 - -0.6 396,734 - -0.7 396,734 - +0.4 396,734 - +1.4 396,734 - +0.0 397,126 - Pf6N2E2_329 0.25 +0.5 397,126 - Pf6N2E2_329 0.25 -0.4 397,126 - Pf6N2E2_329 0.25 -0.2 397,134 - Pf6N2E2_329 0.25 -0.2 397,134 - Pf6N2E2_329 0.25 +1.0 397,171 + Pf6N2E2_329 0.27 +0.3 397,172 - Pf6N2E2_329 0.27 +0.5 397,199 - Pf6N2E2_329 0.29 +0.5 397,199 - Pf6N2E2_329 0.29 +1.1 397,274 - Pf6N2E2_329 0.33 +1.5 397,292 + Pf6N2E2_329 0.34 +1.0 397,729 + Pf6N2E2_329 0.59 +0.4 397,729 + Pf6N2E2_329 0.59 +2.0 397,730 - Pf6N2E2_329 0.59 -0.5 398,248 + Pf6N2E2_329 0.88 +0.0 398,249 - Pf6N2E2_329 0.88 -0.2 398,437 + +0.3 398,438 - -0.0 398,474 + +0.1 398,516 + +0.4 398,516 + -0.5 398,516 + -0.2 398,516 + +0.0 398,516 + -0.4 398,517 - +1.5 398,517 - +0.5 398,517 - +0.1 398,518 + -0.6 398,519 - -0.8 398,623 + -1.5 398,683 + +0.0 398,683 + -0.8 398,683 + -0.8 398,683 + -0.2 398,684 - +0.4 398,684 - +0.7 399,074 + Pf6N2E2_330 0.68 -0.4 399,074 + Pf6N2E2_330 0.68 +1.0 399,075 - Pf6N2E2_330 0.68 +0.5 399,107 + Pf6N2E2_330 0.74 -0.1 399,108 - Pf6N2E2_330 0.74 -0.9 399,108 - Pf6N2E2_330 0.74 -1.9 399,283 + -0.3 399,284 - -0.0 399,307 - +0.7 399,392 + +0.2
Or see this region's nucleotide sequence