Experiment: Rifampicin 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1138 and Pf6N2E2_1139 are separated by 152 nucleotides Pf6N2E2_1139 and Pf6N2E2_1140 are separated by 33 nucleotides Pf6N2E2_1140 and Pf6N2E2_1141 are separated by 1 nucleotides
Pf6N2E2_1138 - Transcriptional regulator, AraC family, at 1,334,575 to 1,335,606
_1138
Pf6N2E2_1139 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 1,335,759 to 1,336,730
_1139
Pf6N2E2_1140 - Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-), at 1,336,764 to 1,337,507
_1140
Pf6N2E2_1141 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 1,337,509 to 1,337,802
_1141
Position (kb)
1335
1336
1337 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1335.004 kb on + strand, within Pf6N2E2_1138 at 1335.004 kb on + strand, within Pf6N2E2_1138 at 1335.005 kb on - strand, within Pf6N2E2_1138 at 1335.005 kb on - strand, within Pf6N2E2_1138 at 1335.013 kb on + strand, within Pf6N2E2_1138 at 1335.013 kb on + strand, within Pf6N2E2_1138 at 1335.014 kb on - strand, within Pf6N2E2_1138 at 1335.533 kb on - strand at 1335.533 kb on - strand at 1335.661 kb on + strand at 1335.674 kb on + strand at 1335.675 kb on - strand at 1335.727 kb on + strand at 1335.727 kb on + strand at 1335.727 kb on + strand at 1335.728 kb on - strand at 1335.728 kb on - strand at 1335.728 kb on - strand at 1335.728 kb on - strand at 1335.733 kb on - strand at 1335.758 kb on + strand at 1335.759 kb on - strand at 1335.865 kb on - strand, within Pf6N2E2_1139 at 1335.867 kb on + strand, within Pf6N2E2_1139 at 1335.867 kb on + strand, within Pf6N2E2_1139 at 1335.868 kb on - strand, within Pf6N2E2_1139 at 1335.868 kb on - strand, within Pf6N2E2_1139 at 1336.053 kb on + strand, within Pf6N2E2_1139 at 1336.054 kb on - strand, within Pf6N2E2_1139 at 1336.072 kb on + strand, within Pf6N2E2_1139 at 1336.072 kb on + strand, within Pf6N2E2_1139 at 1336.073 kb on - strand, within Pf6N2E2_1139 at 1336.174 kb on - strand, within Pf6N2E2_1139 at 1336.266 kb on + strand, within Pf6N2E2_1139 at 1336.305 kb on + strand, within Pf6N2E2_1139 at 1336.305 kb on + strand, within Pf6N2E2_1139 at 1336.306 kb on - strand, within Pf6N2E2_1139 at 1336.306 kb on - strand, within Pf6N2E2_1139 at 1336.416 kb on + strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.417 kb on - strand, within Pf6N2E2_1139 at 1336.418 kb on + strand, within Pf6N2E2_1139 at 1336.497 kb on + strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.498 kb on - strand, within Pf6N2E2_1139 at 1336.707 kb on + strand at 1336.716 kb on + strand at 1336.719 kb on + strand at 1336.720 kb on - strand at 1336.779 kb on + strand at 1336.779 kb on + strand at 1336.780 kb on - strand at 1336.780 kb on - strand at 1336.780 kb on - strand at 1336.783 kb on + strand at 1336.783 kb on + strand at 1336.784 kb on - strand at 1336.817 kb on + strand at 1336.817 kb on + strand at 1336.921 kb on + strand, within Pf6N2E2_1140 at 1336.922 kb on - strand, within Pf6N2E2_1140 at 1336.922 kb on - strand, within Pf6N2E2_1140 at 1337.001 kb on + strand, within Pf6N2E2_1140 at 1337.590 kb on + strand, within Pf6N2E2_1141 at 1337.600 kb on - strand, within Pf6N2E2_1141 at 1337.600 kb on - strand, within Pf6N2E2_1141 at 1337.671 kb on + strand, within Pf6N2E2_1141 at 1337.671 kb on + strand, within Pf6N2E2_1141 at 1337.672 kb on - strand, within Pf6N2E2_1141
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.003 mg/ml remove 1,335,004 + Pf6N2E2_1138 0.42 -0.7 1,335,004 + Pf6N2E2_1138 0.42 +0.8 1,335,005 - Pf6N2E2_1138 0.42 -1.8 1,335,005 - Pf6N2E2_1138 0.42 -0.9 1,335,013 + Pf6N2E2_1138 0.42 +0.4 1,335,013 + Pf6N2E2_1138 0.42 +0.2 1,335,014 - Pf6N2E2_1138 0.43 +0.0 1,335,533 - -0.4 1,335,533 - -0.9 1,335,661 + -0.8 1,335,674 + +1.0 1,335,675 - +2.5 1,335,727 + +0.7 1,335,727 + +0.3 1,335,727 + +0.1 1,335,728 - +0.0 1,335,728 - -0.8 1,335,728 - -1.4 1,335,728 - +1.0 1,335,733 - +0.6 1,335,758 + -0.1 1,335,759 - +1.8 1,335,865 - Pf6N2E2_1139 0.11 +1.3 1,335,867 + Pf6N2E2_1139 0.11 +1.1 1,335,867 + Pf6N2E2_1139 0.11 -1.5 1,335,868 - Pf6N2E2_1139 0.11 -0.2 1,335,868 - Pf6N2E2_1139 0.11 -0.5 1,336,053 + Pf6N2E2_1139 0.30 +0.3 1,336,054 - Pf6N2E2_1139 0.30 -0.8 1,336,072 + Pf6N2E2_1139 0.32 -1.0 1,336,072 + Pf6N2E2_1139 0.32 +0.7 1,336,073 - Pf6N2E2_1139 0.32 +0.5 1,336,174 - Pf6N2E2_1139 0.43 +1.1 1,336,266 + Pf6N2E2_1139 0.52 +0.8 1,336,305 + Pf6N2E2_1139 0.56 +0.4 1,336,305 + Pf6N2E2_1139 0.56 +0.5 1,336,306 - Pf6N2E2_1139 0.56 +1.2 1,336,306 - Pf6N2E2_1139 0.56 +0.1 1,336,416 + Pf6N2E2_1139 0.68 +1.3 1,336,417 - Pf6N2E2_1139 0.68 +0.3 1,336,417 - Pf6N2E2_1139 0.68 +2.0 1,336,417 - Pf6N2E2_1139 0.68 +0.7 1,336,417 - Pf6N2E2_1139 0.68 +0.4 1,336,417 - Pf6N2E2_1139 0.68 +0.6 1,336,418 + Pf6N2E2_1139 0.68 -0.3 1,336,497 + Pf6N2E2_1139 0.76 +0.9 1,336,498 - Pf6N2E2_1139 0.76 -1.1 1,336,498 - Pf6N2E2_1139 0.76 -0.7 1,336,498 - Pf6N2E2_1139 0.76 +0.6 1,336,498 - Pf6N2E2_1139 0.76 +0.1 1,336,707 + -0.3 1,336,716 + -0.7 1,336,719 + +0.9 1,336,720 - -0.6 1,336,779 + +0.8 1,336,779 + +0.0 1,336,780 - -1.1 1,336,780 - -0.3 1,336,780 - -0.4 1,336,783 + -0.2 1,336,783 + +0.3 1,336,784 - -0.2 1,336,817 + +0.5 1,336,817 + -0.4 1,336,921 + Pf6N2E2_1140 0.21 +0.3 1,336,922 - Pf6N2E2_1140 0.21 -0.9 1,336,922 - Pf6N2E2_1140 0.21 +0.7 1,337,001 + Pf6N2E2_1140 0.32 +1.1 1,337,590 + Pf6N2E2_1141 0.28 +1.1 1,337,600 - Pf6N2E2_1141 0.31 +1.0 1,337,600 - Pf6N2E2_1141 0.31 -0.3 1,337,671 + Pf6N2E2_1141 0.55 +0.3 1,337,671 + Pf6N2E2_1141 0.55 -0.2 1,337,672 - Pf6N2E2_1141 0.55 -0.6
Or see this region's nucleotide sequence