Experiment: Rifampicin 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1111 and Pf6N2E2_1112 are separated by 2 nucleotides Pf6N2E2_1112 and Pf6N2E2_1113 are separated by 154 nucleotides Pf6N2E2_1113 and Pf6N2E2_1114 are separated by 567 nucleotides
Pf6N2E2_1111 - cell wall degradation protein, at 1,303,041 to 1,304,567
_1111
Pf6N2E2_1112 - NAD(P)H oxidoreductase YRKL (EC 1.6.99.-) @ Putative NADPH-quinone reductase (modulator of drug activity B) @ Flavodoxin 2, at 1,304,570 to 1,305,199
_1112
Pf6N2E2_1113 - Transcriptional regulator, TetR family, at 1,305,354 to 1,305,905
_1113
Pf6N2E2_1114 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 1,306,473 to 1,309,034
_1114
Position (kb)
1305
1306 Strain fitness (log2 ratio)
-1
0
1 at 1304.505 kb on + strand at 1304.505 kb on + strand at 1304.506 kb on - strand at 1304.506 kb on - strand at 1304.506 kb on - strand at 1304.520 kb on + strand at 1304.520 kb on + strand at 1304.521 kb on - strand at 1304.766 kb on + strand, within Pf6N2E2_1112 at 1305.238 kb on + strand at 1305.239 kb on - strand at 1305.239 kb on - strand at 1305.239 kb on - strand at 1305.249 kb on + strand at 1305.453 kb on + strand, within Pf6N2E2_1113 at 1305.496 kb on - strand, within Pf6N2E2_1113 at 1305.496 kb on - strand, within Pf6N2E2_1113 at 1305.594 kb on + strand, within Pf6N2E2_1113 at 1305.688 kb on - strand, within Pf6N2E2_1113 at 1306.010 kb on + strand at 1306.010 kb on + strand at 1306.011 kb on - strand at 1306.080 kb on + strand at 1306.349 kb on + strand at 1306.349 kb on + strand at 1306.349 kb on + strand at 1306.350 kb on - strand at 1306.626 kb on + strand at 1306.626 kb on + strand at 1306.627 kb on - strand at 1306.651 kb on - strand at 1306.701 kb on + strand at 1306.702 kb on - strand at 1306.798 kb on - strand, within Pf6N2E2_1114 at 1306.878 kb on + strand, within Pf6N2E2_1114 at 1306.879 kb on - strand, within Pf6N2E2_1114 at 1306.879 kb on - strand, within Pf6N2E2_1114
Per-strain Table
Position Strand Gene LocusTag Fraction Rifampicin 0.003 mg/ml remove 1,304,505 + +0.5 1,304,505 + -0.8 1,304,506 - +0.0 1,304,506 - +0.1 1,304,506 - +0.5 1,304,520 + -0.1 1,304,520 + -0.6 1,304,521 - -0.2 1,304,766 + Pf6N2E2_1112 0.31 +0.8 1,305,238 + -1.0 1,305,239 - -0.4 1,305,239 - -0.9 1,305,239 - +0.1 1,305,249 + +1.6 1,305,453 + Pf6N2E2_1113 0.18 -0.1 1,305,496 - Pf6N2E2_1113 0.26 -0.7 1,305,496 - Pf6N2E2_1113 0.26 +0.8 1,305,594 + Pf6N2E2_1113 0.43 -0.6 1,305,688 - Pf6N2E2_1113 0.61 -1.4 1,306,010 + -0.4 1,306,010 + -0.1 1,306,011 - +0.2 1,306,080 + -0.3 1,306,349 + +0.1 1,306,349 + +0.2 1,306,349 + +0.6 1,306,350 - -0.1 1,306,626 + +0.9 1,306,626 + +0.1 1,306,627 - +0.6 1,306,651 - -1.4 1,306,701 + -0.7 1,306,702 - -0.2 1,306,798 - Pf6N2E2_1114 0.13 +0.2 1,306,878 + Pf6N2E2_1114 0.16 +0.6 1,306,879 - Pf6N2E2_1114 0.16 +0.3 1,306,879 - Pf6N2E2_1114 0.16 +1.1
Or see this region's nucleotide sequence