Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06449 and H281DRAFT_06450 are separated by 39 nucleotides H281DRAFT_06450 and H281DRAFT_06451 are separated by 148 nucleotides H281DRAFT_06451 and H281DRAFT_06452 overlap by 8 nucleotides
H281DRAFT_06449: H281DRAFT_06449 - hypothetical protein, at 55,416 to 55,631
_06449
H281DRAFT_06450: H281DRAFT_06450 - ATP-dependent Clp protease ATP-binding subunit ClpC, at 55,671 to 58,448
_06450
H281DRAFT_06451: H281DRAFT_06451 - Uncharacterized protein, linocin/CFP29 family, at 58,597 to 59,400
_06451
H281DRAFT_06452: H281DRAFT_06452 - putative iron-dependent peroxidase, at 59,393 to 60,439
_06452
Position (kb) 55
56
57
58
59 Strain fitness (log2 ratio) -2
-1
0
1 at 54.756 kb on - strand at 54.784 kb on + strand at 54.784 kb on + strand at 54.785 kb on - strand at 54.817 kb on + strand at 54.817 kb on + strand at 55.168 kb on + strand at 55.215 kb on + strand at 55.215 kb on + strand at 55.216 kb on - strand at 55.300 kb on - strand at 55.331 kb on + strand at 55.374 kb on + strand at 55.380 kb on - strand at 55.415 kb on + strand at 55.447 kb on - strand, within H281DRAFT_06449 at 55.447 kb on - strand, within H281DRAFT_06449 at 55.550 kb on + strand, within H281DRAFT_06449 at 55.550 kb on + strand, within H281DRAFT_06449 at 55.550 kb on + strand, within H281DRAFT_06449 at 55.550 kb on + strand, within H281DRAFT_06449 at 55.550 kb on + strand, within H281DRAFT_06449 at 55.551 kb on - strand, within H281DRAFT_06449 at 55.632 kb on + strand at 55.651 kb on + strand at 55.651 kb on + strand at 55.652 kb on - strand at 55.672 kb on + strand at 55.672 kb on + strand at 55.814 kb on - strand at 56.293 kb on - strand, within H281DRAFT_06450 at 56.305 kb on - strand, within H281DRAFT_06450 at 56.360 kb on - strand, within H281DRAFT_06450 at 56.414 kb on - strand, within H281DRAFT_06450 at 56.613 kb on + strand, within H281DRAFT_06450 at 56.717 kb on + strand, within H281DRAFT_06450 at 56.856 kb on - strand, within H281DRAFT_06450 at 57.047 kb on + strand, within H281DRAFT_06450 at 57.047 kb on + strand, within H281DRAFT_06450 at 57.048 kb on - strand, within H281DRAFT_06450 at 57.049 kb on + strand, within H281DRAFT_06450 at 57.223 kb on + strand, within H281DRAFT_06450 at 57.223 kb on + strand, within H281DRAFT_06450 at 57.224 kb on - strand, within H281DRAFT_06450 at 57.224 kb on - strand, within H281DRAFT_06450 at 57.499 kb on - strand, within H281DRAFT_06450 at 57.812 kb on - strand, within H281DRAFT_06450 at 57.886 kb on + strand, within H281DRAFT_06450 at 57.886 kb on + strand, within H281DRAFT_06450 at 57.886 kb on + strand, within H281DRAFT_06450 at 57.886 kb on + strand, within H281DRAFT_06450 at 58.013 kb on + strand, within H281DRAFT_06450 at 58.015 kb on + strand, within H281DRAFT_06450 at 58.015 kb on + strand, within H281DRAFT_06450 at 58.015 kb on + strand, within H281DRAFT_06450 at 58.016 kb on - strand, within H281DRAFT_06450 at 58.090 kb on + strand, within H281DRAFT_06450 at 58.090 kb on + strand, within H281DRAFT_06450 at 58.091 kb on - strand, within H281DRAFT_06450 at 58.260 kb on - strand at 58.566 kb on - strand at 58.668 kb on - strand at 58.668 kb on - strand at 58.690 kb on - strand, within H281DRAFT_06451 at 58.698 kb on + strand, within H281DRAFT_06451 at 58.756 kb on + strand at 58.759 kb on + strand, within H281DRAFT_06451 at 58.869 kb on - strand, within H281DRAFT_06451 at 58.896 kb on - strand, within H281DRAFT_06451 at 59.405 kb on + strand at 59.406 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 54,756 - -0.1 54,784 + -2.2 54,784 + -1.3 54,785 - -1.7 54,817 + +0.6 54,817 + +0.0 55,168 + +1.2 55,215 + +0.1 55,215 + +0.8 55,216 - +0.2 55,300 - +0.0 55,331 + -0.2 55,374 + +0.7 55,380 - +0.2 55,415 + -0.4 55,447 - H281DRAFT_06449 0.14 +0.9 55,447 - H281DRAFT_06449 0.14 +0.6 55,550 + H281DRAFT_06449 0.62 +0.0 55,550 + H281DRAFT_06449 0.62 -0.5 55,550 + H281DRAFT_06449 0.62 +0.1 55,550 + H281DRAFT_06449 0.62 +0.6 55,550 + H281DRAFT_06449 0.62 -0.1 55,551 - H281DRAFT_06449 0.62 +0.6 55,632 + -0.0 55,651 + -0.3 55,651 + +0.1 55,652 - +1.1 55,672 + +0.4 55,672 + -0.7 55,814 - -0.9 56,293 - H281DRAFT_06450 0.22 +0.8 56,305 - H281DRAFT_06450 0.23 -1.3 56,360 - H281DRAFT_06450 0.25 -0.4 56,414 - H281DRAFT_06450 0.27 +1.7 56,613 + H281DRAFT_06450 0.34 +0.4 56,717 + H281DRAFT_06450 0.38 +0.6 56,856 - H281DRAFT_06450 0.43 +1.1 57,047 + H281DRAFT_06450 0.50 -1.2 57,047 + H281DRAFT_06450 0.50 -1.3 57,048 - H281DRAFT_06450 0.50 +1.2 57,049 + H281DRAFT_06450 0.50 -0.3 57,223 + H281DRAFT_06450 0.56 +0.7 57,223 + H281DRAFT_06450 0.56 +0.2 57,224 - H281DRAFT_06450 0.56 +1.0 57,224 - H281DRAFT_06450 0.56 +0.8 57,499 - H281DRAFT_06450 0.66 +1.2 57,812 - H281DRAFT_06450 0.77 -1.0 57,886 + H281DRAFT_06450 0.80 -1.1 57,886 + H281DRAFT_06450 0.80 +0.2 57,886 + H281DRAFT_06450 0.80 +0.1 57,886 + H281DRAFT_06450 0.80 -0.1 58,013 + H281DRAFT_06450 0.84 -0.5 58,015 + H281DRAFT_06450 0.84 +1.1 58,015 + H281DRAFT_06450 0.84 +0.3 58,015 + H281DRAFT_06450 0.84 +1.0 58,016 - H281DRAFT_06450 0.84 -0.9 58,090 + H281DRAFT_06450 0.87 +0.3 58,090 + H281DRAFT_06450 0.87 +0.5 58,091 - H281DRAFT_06450 0.87 +0.7 58,260 - -0.6 58,566 - -0.3 58,668 - -0.1 58,668 - -0.3 58,690 - H281DRAFT_06451 0.12 -0.3 58,698 + H281DRAFT_06451 0.13 +0.0 58,756 + -1.3 58,759 + H281DRAFT_06451 0.20 +0.7 58,869 - H281DRAFT_06451 0.34 +0.4 58,896 - H281DRAFT_06451 0.37 +1.0 59,405 + +1.0 59,406 - +1.1
Or see this region's nucleotide sequence