Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05847 and H281DRAFT_05848 are separated by 347 nucleotides H281DRAFT_05848 and H281DRAFT_05849 are separated by 158 nucleotides H281DRAFT_05849 and H281DRAFT_05850 are separated by 172 nucleotides H281DRAFT_05850 and H281DRAFT_05851 are separated by 84 nucleotides
H281DRAFT_05847: H281DRAFT_05847 - indolepyruvate ferredoxin oxidoreductase, at 125,341 to 128,940
_05847
H281DRAFT_05848: H281DRAFT_05848 - hypothetical protein, at 129,288 to 129,485
_05848
H281DRAFT_05849: H281DRAFT_05849 - 4-hydroxyphenylpyruvate dioxygenase, at 129,644 to 130,741
_05849
H281DRAFT_05850: H281DRAFT_05850 - transcriptional regulator, AsnC family, at 130,914 to 131,411
_05850
H281DRAFT_05851: H281DRAFT_05851 - Protein N-acetyltransferase, RimJ/RimL family, at 131,496 to 132,164
_05851
Position (kb) 129
130
131 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 128.767 kb on - strand at 128.840 kb on + strand at 128.840 kb on + strand at 128.841 kb on - strand at 128.841 kb on - strand at 128.864 kb on + strand at 128.864 kb on + strand at 128.864 kb on + strand at 128.864 kb on + strand at 128.864 kb on + strand at 128.865 kb on - strand at 128.865 kb on - strand at 128.890 kb on + strand at 128.891 kb on - strand at 128.926 kb on + strand at 128.941 kb on + strand at 128.941 kb on + strand at 128.941 kb on + strand at 128.947 kb on + strand at 128.947 kb on + strand at 128.947 kb on + strand at 128.947 kb on + strand at 128.948 kb on - strand at 128.948 kb on - strand at 129.007 kb on + strand at 129.025 kb on - strand at 129.025 kb on - strand at 129.038 kb on - strand at 129.047 kb on + strand at 129.047 kb on + strand at 129.047 kb on + strand at 129.047 kb on + strand at 129.048 kb on - strand at 129.048 kb on - strand at 129.048 kb on - strand at 129.149 kb on + strand at 129.149 kb on + strand at 129.150 kb on - strand at 129.202 kb on - strand at 129.202 kb on - strand at 129.219 kb on + strand at 129.219 kb on + strand at 129.219 kb on + strand at 129.219 kb on + strand at 129.219 kb on + strand at 129.220 kb on - strand at 129.220 kb on - strand at 129.220 kb on - strand at 129.283 kb on + strand at 129.283 kb on + strand at 129.284 kb on - strand at 129.284 kb on - strand at 129.297 kb on - strand at 129.302 kb on + strand at 129.494 kb on + strand at 129.504 kb on + strand at 129.505 kb on - strand at 129.550 kb on - strand at 129.569 kb on + strand at 129.570 kb on - strand at 129.593 kb on - strand at 129.897 kb on + strand, within H281DRAFT_05849 at 129.897 kb on + strand, within H281DRAFT_05849 at 129.897 kb on + strand, within H281DRAFT_05849 at 129.897 kb on + strand, within H281DRAFT_05849 at 129.898 kb on - strand, within H281DRAFT_05849 at 129.900 kb on + strand, within H281DRAFT_05849 at 129.900 kb on + strand, within H281DRAFT_05849 at 129.900 kb on + strand, within H281DRAFT_05849 at 129.901 kb on - strand, within H281DRAFT_05849 at 129.961 kb on - strand, within H281DRAFT_05849 at 129.961 kb on - strand, within H281DRAFT_05849 at 130.063 kb on + strand, within H281DRAFT_05849 at 130.063 kb on + strand, within H281DRAFT_05849 at 130.064 kb on - strand, within H281DRAFT_05849 at 130.119 kb on - strand, within H281DRAFT_05849 at 130.140 kb on + strand, within H281DRAFT_05849 at 130.141 kb on - strand, within H281DRAFT_05849 at 130.141 kb on - strand, within H281DRAFT_05849 at 130.174 kb on - strand, within H281DRAFT_05849 at 130.232 kb on - strand, within H281DRAFT_05849 at 130.233 kb on + strand, within H281DRAFT_05849 at 130.233 kb on + strand, within H281DRAFT_05849 at 130.233 kb on + strand, within H281DRAFT_05849 at 130.234 kb on - strand, within H281DRAFT_05849 at 130.234 kb on - strand, within H281DRAFT_05849 at 130.234 kb on - strand, within H281DRAFT_05849 at 130.234 kb on - strand, within H281DRAFT_05849 at 130.234 kb on - strand, within H281DRAFT_05849 at 130.234 kb on - strand, within H281DRAFT_05849 at 130.296 kb on + strand, within H281DRAFT_05849 at 130.296 kb on + strand, within H281DRAFT_05849 at 130.297 kb on - strand, within H281DRAFT_05849 at 130.297 kb on - strand, within H281DRAFT_05849 at 130.301 kb on - strand, within H281DRAFT_05849 at 130.362 kb on + strand, within H281DRAFT_05849 at 130.362 kb on + strand, within H281DRAFT_05849 at 130.573 kb on + strand, within H281DRAFT_05849 at 130.573 kb on + strand, within H281DRAFT_05849 at 130.574 kb on - strand, within H281DRAFT_05849 at 130.592 kb on + strand, within H281DRAFT_05849 at 130.592 kb on + strand, within H281DRAFT_05849 at 130.593 kb on - strand, within H281DRAFT_05849 at 130.593 kb on - strand, within H281DRAFT_05849 at 130.593 kb on - strand, within H281DRAFT_05849 at 130.593 kb on - strand, within H281DRAFT_05849 at 130.593 kb on - strand, within H281DRAFT_05849 at 130.593 kb on - strand, within H281DRAFT_05849 at 130.593 kb on - strand, within H281DRAFT_05849 at 130.593 kb on - strand at 130.593 kb on - strand, within H281DRAFT_05849 at 130.606 kb on + strand, within H281DRAFT_05849 at 130.606 kb on + strand, within H281DRAFT_05849 at 130.683 kb on + strand at 130.884 kb on + strand at 130.924 kb on + strand at 130.930 kb on + strand at 130.949 kb on + strand at 131.041 kb on + strand, within H281DRAFT_05850 at 131.076 kb on + strand, within H281DRAFT_05850 at 131.077 kb on - strand, within H281DRAFT_05850 at 131.124 kb on + strand, within H281DRAFT_05850 at 131.124 kb on + strand, within H281DRAFT_05850 at 131.125 kb on - strand, within H281DRAFT_05850 at 131.125 kb on - strand, within H281DRAFT_05850 at 131.125 kb on - strand, within H281DRAFT_05850 at 131.125 kb on - strand, within H281DRAFT_05850 at 131.125 kb on - strand, within H281DRAFT_05850 at 131.125 kb on - strand, within H281DRAFT_05850 at 131.246 kb on + strand, within H281DRAFT_05850 at 131.246 kb on + strand, within H281DRAFT_05850 at 131.247 kb on - strand, within H281DRAFT_05850 at 131.247 kb on - strand, within H281DRAFT_05850 at 131.265 kb on + strand, within H281DRAFT_05850 at 131.266 kb on - strand, within H281DRAFT_05850 at 131.266 kb on - strand, within H281DRAFT_05850 at 131.266 kb on - strand, within H281DRAFT_05850 at 131.278 kb on + strand, within H281DRAFT_05850 at 131.279 kb on - strand, within H281DRAFT_05850 at 131.570 kb on - strand, within H281DRAFT_05851 at 131.645 kb on - strand, within H281DRAFT_05851 at 131.737 kb on + strand, within H281DRAFT_05851 at 131.737 kb on + strand, within H281DRAFT_05851 at 131.737 kb on + strand, within H281DRAFT_05851 at 131.738 kb on - strand, within H281DRAFT_05851 at 131.738 kb on - strand, within H281DRAFT_05851 at 131.738 kb on - strand, within H281DRAFT_05851 at 131.738 kb on - strand, within H281DRAFT_05851 at 131.738 kb on - strand, within H281DRAFT_05851 at 131.738 kb on - strand, within H281DRAFT_05851 at 131.738 kb on - strand, within H281DRAFT_05851 at 131.738 kb on - strand, within H281DRAFT_05851 at 131.738 kb on - strand, within H281DRAFT_05851
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 128,767 - +0.4 128,840 + +0.3 128,840 + -0.7 128,841 - +0.7 128,841 - +0.7 128,864 + +0.5 128,864 + +0.9 128,864 + -1.3 128,864 + +1.1 128,864 + +0.1 128,865 - +1.0 128,865 - +0.4 128,890 + -0.1 128,891 - +0.0 128,926 + -0.6 128,941 + -0.1 128,941 + -0.0 128,941 + +0.2 128,947 + -0.5 128,947 + +0.9 128,947 + -0.2 128,947 + -0.6 128,948 - -0.1 128,948 - +0.1 129,007 + +0.3 129,025 - +0.1 129,025 - -0.0 129,038 - +0.5 129,047 + -0.5 129,047 + +0.5 129,047 + +0.1 129,047 + -1.3 129,048 - +0.6 129,048 - -0.2 129,048 - -0.8 129,149 + -0.5 129,149 + -0.2 129,150 - +0.3 129,202 - -0.3 129,202 - -0.4 129,219 + -1.0 129,219 + -0.5 129,219 + -0.5 129,219 + +0.9 129,219 + -0.0 129,220 - -1.4 129,220 - -1.3 129,220 - -0.1 129,283 + +0.9 129,283 + +0.8 129,284 - -1.2 129,284 - +0.2 129,297 - -0.3 129,302 + +0.0 129,494 + +0.3 129,504 + +0.2 129,505 - +0.3 129,550 - +0.2 129,569 + -0.6 129,570 - -0.1 129,593 - -0.1 129,897 + H281DRAFT_05849 0.23 -0.6 129,897 + H281DRAFT_05849 0.23 +0.9 129,897 + H281DRAFT_05849 0.23 -0.1 129,897 + H281DRAFT_05849 0.23 +0.2 129,898 - H281DRAFT_05849 0.23 +0.8 129,900 + H281DRAFT_05849 0.23 +0.1 129,900 + H281DRAFT_05849 0.23 +0.9 129,900 + H281DRAFT_05849 0.23 -0.1 129,901 - H281DRAFT_05849 0.23 -1.1 129,961 - H281DRAFT_05849 0.29 -0.0 129,961 - H281DRAFT_05849 0.29 +0.2 130,063 + H281DRAFT_05849 0.38 +0.1 130,063 + H281DRAFT_05849 0.38 -0.3 130,064 - H281DRAFT_05849 0.38 +0.3 130,119 - H281DRAFT_05849 0.43 +0.4 130,140 + H281DRAFT_05849 0.45 +0.4 130,141 - H281DRAFT_05849 0.45 +1.5 130,141 - H281DRAFT_05849 0.45 +0.5 130,174 - H281DRAFT_05849 0.48 +0.5 130,232 - H281DRAFT_05849 0.54 -0.1 130,233 + H281DRAFT_05849 0.54 -0.1 130,233 + H281DRAFT_05849 0.54 -0.1 130,233 + H281DRAFT_05849 0.54 -0.1 130,234 - H281DRAFT_05849 0.54 +0.4 130,234 - H281DRAFT_05849 0.54 -0.9 130,234 - H281DRAFT_05849 0.54 +0.2 130,234 - H281DRAFT_05849 0.54 -0.7 130,234 - H281DRAFT_05849 0.54 +0.2 130,234 - H281DRAFT_05849 0.54 +0.1 130,296 + H281DRAFT_05849 0.59 +1.1 130,296 + H281DRAFT_05849 0.59 +1.6 130,297 - H281DRAFT_05849 0.59 +0.3 130,297 - H281DRAFT_05849 0.59 -0.4 130,301 - H281DRAFT_05849 0.60 +0.1 130,362 + H281DRAFT_05849 0.65 +0.0 130,362 + H281DRAFT_05849 0.65 +0.5 130,573 + H281DRAFT_05849 0.85 -0.1 130,573 + H281DRAFT_05849 0.85 -0.1 130,574 - H281DRAFT_05849 0.85 +0.1 130,592 + H281DRAFT_05849 0.86 +0.4 130,592 + H281DRAFT_05849 0.86 -0.0 130,593 - H281DRAFT_05849 0.86 -0.1 130,593 - H281DRAFT_05849 0.86 +0.2 130,593 - H281DRAFT_05849 0.86 -0.0 130,593 - H281DRAFT_05849 0.86 -0.3 130,593 - H281DRAFT_05849 0.86 +0.3 130,593 - H281DRAFT_05849 0.86 -1.2 130,593 - H281DRAFT_05849 0.86 +0.1 130,593 - +1.6 130,593 - H281DRAFT_05849 0.86 -0.2 130,606 + H281DRAFT_05849 0.88 -0.5 130,606 + H281DRAFT_05849 0.88 +1.6 130,683 + +0.3 130,884 + +0.8 130,924 + +0.0 130,930 + -1.2 130,949 + -0.8 131,041 + H281DRAFT_05850 0.26 +0.2 131,076 + H281DRAFT_05850 0.33 +0.1 131,077 - H281DRAFT_05850 0.33 +0.6 131,124 + H281DRAFT_05850 0.42 +0.7 131,124 + H281DRAFT_05850 0.42 -3.1 131,125 - H281DRAFT_05850 0.42 -0.3 131,125 - H281DRAFT_05850 0.42 -0.7 131,125 - H281DRAFT_05850 0.42 -0.1 131,125 - H281DRAFT_05850 0.42 +0.1 131,125 - H281DRAFT_05850 0.42 +0.2 131,125 - H281DRAFT_05850 0.42 +1.1 131,246 + H281DRAFT_05850 0.67 -1.6 131,246 + H281DRAFT_05850 0.67 +0.2 131,247 - H281DRAFT_05850 0.67 -1.1 131,247 - H281DRAFT_05850 0.67 -0.2 131,265 + H281DRAFT_05850 0.70 -0.9 131,266 - H281DRAFT_05850 0.71 -0.2 131,266 - H281DRAFT_05850 0.71 -0.7 131,266 - H281DRAFT_05850 0.71 -0.3 131,278 + H281DRAFT_05850 0.73 -0.6 131,279 - H281DRAFT_05850 0.73 -0.2 131,570 - H281DRAFT_05851 0.11 +0.6 131,645 - H281DRAFT_05851 0.22 -0.5 131,737 + H281DRAFT_05851 0.36 +0.5 131,737 + H281DRAFT_05851 0.36 +0.4 131,737 + H281DRAFT_05851 0.36 +1.2 131,738 - H281DRAFT_05851 0.36 -0.0 131,738 - H281DRAFT_05851 0.36 +0.1 131,738 - H281DRAFT_05851 0.36 +0.1 131,738 - H281DRAFT_05851 0.36 +0.3 131,738 - H281DRAFT_05851 0.36 +0.2 131,738 - H281DRAFT_05851 0.36 -0.9 131,738 - H281DRAFT_05851 0.36 +1.1 131,738 - H281DRAFT_05851 0.36 -0.4 131,738 - H281DRAFT_05851 0.36 +0.8
Or see this region's nucleotide sequence