Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05846 and H281DRAFT_05847 are separated by 155 nucleotides H281DRAFT_05847 and H281DRAFT_05848 are separated by 347 nucleotides H281DRAFT_05848 and H281DRAFT_05849 are separated by 158 nucleotides
H281DRAFT_05846: H281DRAFT_05846 - malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+), at 122,903 to 125,185
_05846
H281DRAFT_05847: H281DRAFT_05847 - indolepyruvate ferredoxin oxidoreductase, at 125,341 to 128,940
_05847
H281DRAFT_05848: H281DRAFT_05848 - hypothetical protein, at 129,288 to 129,485
_05848
H281DRAFT_05849: H281DRAFT_05849 - 4-hydroxyphenylpyruvate dioxygenase, at 129,644 to 130,741
_05849
Position (kb) 125
126
127
128
129 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 124.419 kb on - strand, within H281DRAFT_05846 at 124.690 kb on + strand, within H281DRAFT_05846 at 124.694 kb on + strand, within H281DRAFT_05846 at 124.694 kb on + strand, within H281DRAFT_05846 at 124.694 kb on + strand, within H281DRAFT_05846 at 124.694 kb on + strand, within H281DRAFT_05846 at 124.695 kb on - strand, within H281DRAFT_05846 at 124.695 kb on - strand, within H281DRAFT_05846 at 124.695 kb on - strand, within H281DRAFT_05846 at 125.018 kb on + strand at 125.019 kb on - strand at 125.019 kb on - strand at 125.184 kb on - strand at 125.184 kb on - strand at 125.666 kb on + strand at 125.666 kb on + strand at 125.667 kb on - strand at 125.667 kb on - strand at 125.667 kb on - strand at 125.667 kb on - strand at 125.730 kb on + strand, within H281DRAFT_05847 at 125.731 kb on - strand, within H281DRAFT_05847 at 125.792 kb on + strand, within H281DRAFT_05847 at 125.810 kb on + strand, within H281DRAFT_05847 at 125.810 kb on + strand, within H281DRAFT_05847 at 125.811 kb on - strand, within H281DRAFT_05847 at 125.811 kb on - strand, within H281DRAFT_05847 at 125.823 kb on - strand, within H281DRAFT_05847 at 125.945 kb on + strand, within H281DRAFT_05847 at 125.946 kb on - strand, within H281DRAFT_05847 at 125.972 kb on + strand, within H281DRAFT_05847 at 126.015 kb on + strand, within H281DRAFT_05847 at 126.015 kb on + strand, within H281DRAFT_05847 at 126.016 kb on - strand, within H281DRAFT_05847 at 126.081 kb on - strand, within H281DRAFT_05847 at 126.081 kb on - strand, within H281DRAFT_05847 at 126.171 kb on + strand, within H281DRAFT_05847 at 126.192 kb on - strand, within H281DRAFT_05847 at 126.192 kb on - strand, within H281DRAFT_05847 at 126.192 kb on - strand, within H281DRAFT_05847 at 126.192 kb on - strand, within H281DRAFT_05847 at 126.297 kb on + strand, within H281DRAFT_05847 at 126.298 kb on - strand, within H281DRAFT_05847 at 126.702 kb on - strand, within H281DRAFT_05847 at 126.887 kb on + strand, within H281DRAFT_05847 at 126.888 kb on - strand, within H281DRAFT_05847 at 127.046 kb on + strand, within H281DRAFT_05847 at 127.046 kb on + strand, within H281DRAFT_05847 at 127.046 kb on + strand, within H281DRAFT_05847 at 127.047 kb on - strand, within H281DRAFT_05847 at 127.154 kb on + strand, within H281DRAFT_05847 at 127.155 kb on - strand, within H281DRAFT_05847 at 127.284 kb on - strand, within H281DRAFT_05847 at 127.284 kb on - strand, within H281DRAFT_05847 at 127.284 kb on - strand, within H281DRAFT_05847 at 127.295 kb on + strand, within H281DRAFT_05847 at 127.295 kb on + strand, within H281DRAFT_05847 at 127.296 kb on - strand, within H281DRAFT_05847 at 127.296 kb on - strand, within H281DRAFT_05847 at 127.299 kb on + strand, within H281DRAFT_05847 at 127.299 kb on + strand, within H281DRAFT_05847 at 127.299 kb on + strand, within H281DRAFT_05847 at 127.299 kb on + strand, within H281DRAFT_05847 at 127.299 kb on + strand, within H281DRAFT_05847 at 127.299 kb on + strand, within H281DRAFT_05847 at 127.300 kb on - strand, within H281DRAFT_05847 at 127.300 kb on - strand, within H281DRAFT_05847 at 127.300 kb on - strand, within H281DRAFT_05847 at 127.355 kb on + strand, within H281DRAFT_05847 at 127.356 kb on - strand, within H281DRAFT_05847 at 127.484 kb on + strand, within H281DRAFT_05847 at 127.485 kb on - strand, within H281DRAFT_05847 at 127.485 kb on - strand, within H281DRAFT_05847 at 127.485 kb on - strand, within H281DRAFT_05847 at 127.720 kb on + strand, within H281DRAFT_05847 at 127.720 kb on + strand, within H281DRAFT_05847 at 127.721 kb on - strand, within H281DRAFT_05847 at 127.838 kb on + strand, within H281DRAFT_05847 at 127.838 kb on + strand, within H281DRAFT_05847 at 127.838 kb on + strand, within H281DRAFT_05847 at 127.838 kb on + strand, within H281DRAFT_05847 at 127.838 kb on + strand, within H281DRAFT_05847 at 127.838 kb on + strand, within H281DRAFT_05847 at 127.839 kb on - strand, within H281DRAFT_05847 at 127.839 kb on - strand, within H281DRAFT_05847 at 127.964 kb on + strand, within H281DRAFT_05847 at 127.964 kb on + strand, within H281DRAFT_05847 at 127.965 kb on - strand, within H281DRAFT_05847 at 127.965 kb on - strand, within H281DRAFT_05847 at 127.965 kb on - strand, within H281DRAFT_05847 at 127.965 kb on - strand, within H281DRAFT_05847 at 127.972 kb on + strand, within H281DRAFT_05847 at 127.972 kb on + strand, within H281DRAFT_05847 at 128.039 kb on + strand, within H281DRAFT_05847 at 128.040 kb on - strand, within H281DRAFT_05847 at 128.040 kb on - strand, within H281DRAFT_05847 at 128.040 kb on - strand, within H281DRAFT_05847 at 128.121 kb on - strand, within H281DRAFT_05847 at 128.136 kb on - strand, within H281DRAFT_05847 at 128.363 kb on + strand, within H281DRAFT_05847 at 128.364 kb on - strand, within H281DRAFT_05847 at 128.586 kb on - strand at 128.601 kb on - strand at 128.767 kb on - strand at 128.840 kb on + strand at 128.840 kb on + strand at 128.841 kb on - strand at 128.841 kb on - strand at 128.864 kb on + strand at 128.864 kb on + strand at 128.864 kb on + strand at 128.864 kb on + strand at 128.864 kb on + strand at 128.865 kb on - strand at 128.865 kb on - strand at 128.890 kb on + strand at 128.891 kb on - strand at 128.926 kb on + strand at 128.941 kb on + strand at 128.941 kb on + strand at 128.941 kb on + strand at 128.947 kb on + strand at 128.947 kb on + strand at 128.947 kb on + strand at 128.947 kb on + strand at 128.948 kb on - strand at 128.948 kb on - strand at 129.007 kb on + strand at 129.025 kb on - strand at 129.025 kb on - strand at 129.038 kb on - strand at 129.047 kb on + strand at 129.047 kb on + strand at 129.047 kb on + strand at 129.047 kb on + strand at 129.048 kb on - strand at 129.048 kb on - strand at 129.048 kb on - strand at 129.149 kb on + strand at 129.149 kb on + strand at 129.150 kb on - strand at 129.202 kb on - strand at 129.202 kb on - strand at 129.219 kb on + strand at 129.219 kb on + strand at 129.219 kb on + strand at 129.219 kb on + strand at 129.219 kb on + strand at 129.220 kb on - strand at 129.220 kb on - strand at 129.220 kb on - strand at 129.283 kb on + strand at 129.283 kb on + strand at 129.284 kb on - strand at 129.284 kb on - strand at 129.297 kb on - strand at 129.302 kb on + strand at 129.494 kb on + strand at 129.504 kb on + strand at 129.505 kb on - strand at 129.550 kb on - strand at 129.569 kb on + strand at 129.570 kb on - strand at 129.593 kb on - strand at 129.897 kb on + strand, within H281DRAFT_05849 at 129.897 kb on + strand, within H281DRAFT_05849 at 129.897 kb on + strand, within H281DRAFT_05849 at 129.897 kb on + strand, within H281DRAFT_05849 at 129.898 kb on - strand, within H281DRAFT_05849 at 129.900 kb on + strand, within H281DRAFT_05849 at 129.900 kb on + strand, within H281DRAFT_05849 at 129.900 kb on + strand, within H281DRAFT_05849 at 129.901 kb on - strand, within H281DRAFT_05849
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 124,419 - H281DRAFT_05846 0.66 -0.3 124,690 + H281DRAFT_05846 0.78 -0.7 124,694 + H281DRAFT_05846 0.78 -0.0 124,694 + H281DRAFT_05846 0.78 -1.2 124,694 + H281DRAFT_05846 0.78 +0.4 124,694 + H281DRAFT_05846 0.78 -0.2 124,695 - H281DRAFT_05846 0.78 -0.4 124,695 - H281DRAFT_05846 0.78 -0.5 124,695 - H281DRAFT_05846 0.78 +0.1 125,018 + +0.5 125,019 - +0.2 125,019 - +0.5 125,184 - +0.2 125,184 - -1.9 125,666 + +0.4 125,666 + -1.8 125,667 - +0.4 125,667 - +0.3 125,667 - +1.5 125,667 - +0.2 125,730 + H281DRAFT_05847 0.11 +0.6 125,731 - H281DRAFT_05847 0.11 -0.2 125,792 + H281DRAFT_05847 0.13 +0.6 125,810 + H281DRAFT_05847 0.13 +0.3 125,810 + H281DRAFT_05847 0.13 -1.1 125,811 - H281DRAFT_05847 0.13 +0.2 125,811 - H281DRAFT_05847 0.13 +0.5 125,823 - H281DRAFT_05847 0.13 -1.1 125,945 + H281DRAFT_05847 0.17 -1.1 125,946 - H281DRAFT_05847 0.17 -0.8 125,972 + H281DRAFT_05847 0.18 -0.1 126,015 + H281DRAFT_05847 0.19 -3.1 126,015 + H281DRAFT_05847 0.19 -1.0 126,016 - H281DRAFT_05847 0.19 -0.3 126,081 - H281DRAFT_05847 0.21 +0.0 126,081 - H281DRAFT_05847 0.21 +0.3 126,171 + H281DRAFT_05847 0.23 -0.0 126,192 - H281DRAFT_05847 0.24 +0.3 126,192 - H281DRAFT_05847 0.24 -0.5 126,192 - H281DRAFT_05847 0.24 +1.0 126,192 - H281DRAFT_05847 0.24 -1.5 126,297 + H281DRAFT_05847 0.27 -0.4 126,298 - H281DRAFT_05847 0.27 -0.9 126,702 - H281DRAFT_05847 0.38 +0.1 126,887 + H281DRAFT_05847 0.43 -0.1 126,888 - H281DRAFT_05847 0.43 +0.6 127,046 + H281DRAFT_05847 0.47 -0.5 127,046 + H281DRAFT_05847 0.47 -0.3 127,046 + H281DRAFT_05847 0.47 -0.2 127,047 - H281DRAFT_05847 0.47 -1.5 127,154 + H281DRAFT_05847 0.50 -0.1 127,155 - H281DRAFT_05847 0.50 -2.0 127,284 - H281DRAFT_05847 0.54 +1.1 127,284 - H281DRAFT_05847 0.54 -0.8 127,284 - H281DRAFT_05847 0.54 -0.4 127,295 + H281DRAFT_05847 0.54 -0.3 127,295 + H281DRAFT_05847 0.54 +1.1 127,296 - H281DRAFT_05847 0.54 -0.2 127,296 - H281DRAFT_05847 0.54 +0.6 127,299 + H281DRAFT_05847 0.54 +0.1 127,299 + H281DRAFT_05847 0.54 +0.6 127,299 + H281DRAFT_05847 0.54 -0.5 127,299 + H281DRAFT_05847 0.54 +0.9 127,299 + H281DRAFT_05847 0.54 +0.4 127,299 + H281DRAFT_05847 0.54 -0.5 127,300 - H281DRAFT_05847 0.54 +0.6 127,300 - H281DRAFT_05847 0.54 +0.1 127,300 - H281DRAFT_05847 0.54 +0.2 127,355 + H281DRAFT_05847 0.56 +0.3 127,356 - H281DRAFT_05847 0.56 -1.1 127,484 + H281DRAFT_05847 0.60 -0.4 127,485 - H281DRAFT_05847 0.60 -1.1 127,485 - H281DRAFT_05847 0.60 -0.0 127,485 - H281DRAFT_05847 0.60 +0.1 127,720 + H281DRAFT_05847 0.66 -0.2 127,720 + H281DRAFT_05847 0.66 +0.3 127,721 - H281DRAFT_05847 0.66 +0.1 127,838 + H281DRAFT_05847 0.69 +0.4 127,838 + H281DRAFT_05847 0.69 -1.6 127,838 + H281DRAFT_05847 0.69 +0.6 127,838 + H281DRAFT_05847 0.69 -0.6 127,838 + H281DRAFT_05847 0.69 -0.5 127,838 + H281DRAFT_05847 0.69 -0.2 127,839 - H281DRAFT_05847 0.69 -0.1 127,839 - H281DRAFT_05847 0.69 +0.8 127,964 + H281DRAFT_05847 0.73 -0.9 127,964 + H281DRAFT_05847 0.73 +0.2 127,965 - H281DRAFT_05847 0.73 -0.5 127,965 - H281DRAFT_05847 0.73 +0.3 127,965 - H281DRAFT_05847 0.73 +0.3 127,965 - H281DRAFT_05847 0.73 +0.6 127,972 + H281DRAFT_05847 0.73 -0.9 127,972 + H281DRAFT_05847 0.73 -0.4 128,039 + H281DRAFT_05847 0.75 -0.1 128,040 - H281DRAFT_05847 0.75 +0.8 128,040 - H281DRAFT_05847 0.75 +0.1 128,040 - H281DRAFT_05847 0.75 -0.0 128,121 - H281DRAFT_05847 0.77 +0.7 128,136 - H281DRAFT_05847 0.78 -1.0 128,363 + H281DRAFT_05847 0.84 +1.2 128,364 - H281DRAFT_05847 0.84 -0.4 128,586 - -0.3 128,601 - -0.0 128,767 - +0.4 128,840 + +0.3 128,840 + -0.7 128,841 - +0.7 128,841 - +0.7 128,864 + +0.5 128,864 + +0.9 128,864 + -1.3 128,864 + +1.1 128,864 + +0.1 128,865 - +1.0 128,865 - +0.4 128,890 + -0.1 128,891 - +0.0 128,926 + -0.6 128,941 + -0.1 128,941 + -0.0 128,941 + +0.2 128,947 + -0.5 128,947 + +0.9 128,947 + -0.2 128,947 + -0.6 128,948 - -0.1 128,948 - +0.1 129,007 + +0.3 129,025 - +0.1 129,025 - -0.0 129,038 - +0.5 129,047 + -0.5 129,047 + +0.5 129,047 + +0.1 129,047 + -1.3 129,048 - +0.6 129,048 - -0.2 129,048 - -0.8 129,149 + -0.5 129,149 + -0.2 129,150 - +0.3 129,202 - -0.3 129,202 - -0.4 129,219 + -1.0 129,219 + -0.5 129,219 + -0.5 129,219 + +0.9 129,219 + -0.0 129,220 - -1.4 129,220 - -1.3 129,220 - -0.1 129,283 + +0.9 129,283 + +0.8 129,284 - -1.2 129,284 - +0.2 129,297 - -0.3 129,302 + +0.0 129,494 + +0.3 129,504 + +0.2 129,505 - +0.3 129,550 - +0.2 129,569 + -0.6 129,570 - -0.1 129,593 - -0.1 129,897 + H281DRAFT_05849 0.23 -0.6 129,897 + H281DRAFT_05849 0.23 +0.9 129,897 + H281DRAFT_05849 0.23 -0.1 129,897 + H281DRAFT_05849 0.23 +0.2 129,898 - H281DRAFT_05849 0.23 +0.8 129,900 + H281DRAFT_05849 0.23 +0.1 129,900 + H281DRAFT_05849 0.23 +0.9 129,900 + H281DRAFT_05849 0.23 -0.1 129,901 - H281DRAFT_05849 0.23 -1.1
Or see this region's nucleotide sequence