Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05446 and H281DRAFT_05447 are separated by 386 nucleotides H281DRAFT_05447 and H281DRAFT_05448 are separated by 89 nucleotides H281DRAFT_05448 and H281DRAFT_05449 are separated by 243 nucleotides
H281DRAFT_05446: H281DRAFT_05446 - Nitrate/nitrite transporter NarK, at 25,065 to 26,423
_05446
H281DRAFT_05447: H281DRAFT_05447 - hypothetical protein, at 26,810 to 27,532
_05447
H281DRAFT_05448: H281DRAFT_05448 - hypothetical protein, at 27,622 to 27,990
_05448
H281DRAFT_05449: H281DRAFT_05449 - Methyl-accepting chemotaxis protein, at 28,234 to 30,192
_05449
Position (kb) 26
27
28 Strain fitness (log2 ratio) -1
0
1 at 25.822 kb on - strand, within H281DRAFT_05446 at 25.828 kb on - strand, within H281DRAFT_05446 at 25.860 kb on - strand, within H281DRAFT_05446 at 26.187 kb on - strand, within H281DRAFT_05446 at 26.218 kb on + strand, within H281DRAFT_05446 at 26.254 kb on - strand, within H281DRAFT_05446 at 26.254 kb on - strand, within H281DRAFT_05446 at 26.523 kb on + strand at 27.331 kb on + strand, within H281DRAFT_05447 at 27.627 kb on + strand at 27.633 kb on + strand at 27.960 kb on - strand at 28.054 kb on - strand at 28.236 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 25,822 - H281DRAFT_05446 0.56 +0.1 25,828 - H281DRAFT_05446 0.56 +0.3 25,860 - H281DRAFT_05446 0.58 +0.1 26,187 - H281DRAFT_05446 0.83 -0.1 26,218 + H281DRAFT_05446 0.85 -0.5 26,254 - H281DRAFT_05446 0.87 +0.3 26,254 - H281DRAFT_05446 0.87 -0.1 26,523 + +0.7 27,331 + H281DRAFT_05447 0.72 +1.0 27,627 + +0.7 27,633 + -1.7 27,960 - -0.8 28,054 - +0.5 28,236 - +0.7
Or see this region's nucleotide sequence