Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05434 and H281DRAFT_05435 are separated by 934 nucleotides H281DRAFT_05435 and H281DRAFT_05436 are separated by 224 nucleotides
H281DRAFT_05434: H281DRAFT_05434 - Predicted amidohydrolase, at 10,720 to 11,616
_05434
H281DRAFT_05435: H281DRAFT_05435 - DNA-binding transcriptional regulator, LysR family, at 12,551 to 13,495
_05435
H281DRAFT_05436: H281DRAFT_05436 - Malate/lactate/ureidoglycolate dehydrogenase, LDH2 family, at 13,720 to 14,712
_05436
Position (kb) 12
13
14 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 11.574 kb on + strand at 11.575 kb on - strand at 11.575 kb on - strand at 11.674 kb on - strand at 11.677 kb on + strand at 11.678 kb on - strand at 11.678 kb on - strand at 11.687 kb on + strand at 11.688 kb on - strand at 11.724 kb on + strand at 11.724 kb on + strand at 11.798 kb on + strand at 11.799 kb on - strand at 11.910 kb on - strand at 11.980 kb on + strand at 12.023 kb on + strand at 12.293 kb on - strand at 12.547 kb on + strand at 12.547 kb on + strand at 12.552 kb on + strand at 12.552 kb on + strand at 12.553 kb on - strand at 12.553 kb on - strand at 12.555 kb on + strand at 12.556 kb on - strand at 12.628 kb on + strand at 12.659 kb on + strand, within H281DRAFT_05435 at 12.680 kb on + strand, within H281DRAFT_05435 at 12.680 kb on + strand, within H281DRAFT_05435 at 12.681 kb on - strand, within H281DRAFT_05435 at 12.681 kb on - strand, within H281DRAFT_05435 at 12.700 kb on - strand, within H281DRAFT_05435 at 12.700 kb on - strand, within H281DRAFT_05435 at 12.732 kb on - strand, within H281DRAFT_05435 at 12.903 kb on - strand, within H281DRAFT_05435 at 12.966 kb on - strand, within H281DRAFT_05435 at 13.196 kb on + strand, within H281DRAFT_05435 at 13.251 kb on + strand, within H281DRAFT_05435 at 13.268 kb on - strand, within H281DRAFT_05435 at 13.394 kb on - strand, within H281DRAFT_05435 at 13.496 kb on - strand at 13.653 kb on + strand at 13.703 kb on + strand at 13.814 kb on - strand at 13.816 kb on - strand at 13.869 kb on - strand, within H281DRAFT_05436 at 14.040 kb on + strand, within H281DRAFT_05436 at 14.041 kb on - strand at 14.041 kb on - strand, within H281DRAFT_05436 at 14.124 kb on - strand, within H281DRAFT_05436 at 14.184 kb on + strand, within H281DRAFT_05436 at 14.358 kb on + strand, within H281DRAFT_05436 at 14.358 kb on + strand, within H281DRAFT_05436 at 14.449 kb on - strand, within H281DRAFT_05436 at 14.468 kb on - strand, within H281DRAFT_05436 at 14.473 kb on - strand, within H281DRAFT_05436
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 11,574 + +0.3 11,575 - -0.3 11,575 - +0.5 11,674 - -0.4 11,677 + +0.3 11,678 - +1.2 11,678 - +0.3 11,687 + +0.0 11,688 - +0.3 11,724 + +0.5 11,724 + +0.1 11,798 + +0.5 11,799 - +0.1 11,910 - +1.0 11,980 + -0.3 12,023 + +0.9 12,293 - +0.6 12,547 + +0.9 12,547 + +0.3 12,552 + -0.0 12,552 + -0.1 12,553 - +0.3 12,553 - -0.3 12,555 + +0.3 12,556 - +0.4 12,628 + -0.3 12,659 + H281DRAFT_05435 0.11 -0.6 12,680 + H281DRAFT_05435 0.14 -0.3 12,680 + H281DRAFT_05435 0.14 -0.6 12,681 - H281DRAFT_05435 0.14 -0.3 12,681 - H281DRAFT_05435 0.14 +0.3 12,700 - H281DRAFT_05435 0.16 -0.9 12,700 - H281DRAFT_05435 0.16 -2.9 12,732 - H281DRAFT_05435 0.19 +1.2 12,903 - H281DRAFT_05435 0.37 -0.2 12,966 - H281DRAFT_05435 0.44 +0.4 13,196 + H281DRAFT_05435 0.68 -1.0 13,251 + H281DRAFT_05435 0.74 -0.1 13,268 - H281DRAFT_05435 0.76 +1.3 13,394 - H281DRAFT_05435 0.89 +0.7 13,496 - -0.5 13,653 + -0.5 13,703 + -1.0 13,814 - -0.2 13,816 - +0.7 13,869 - H281DRAFT_05436 0.15 +0.7 14,040 + H281DRAFT_05436 0.32 -0.4 14,041 - +1.0 14,041 - H281DRAFT_05436 0.32 -0.3 14,124 - H281DRAFT_05436 0.41 -0.0 14,184 + H281DRAFT_05436 0.47 +0.4 14,358 + H281DRAFT_05436 0.64 -0.1 14,358 + H281DRAFT_05436 0.64 -0.2 14,449 - H281DRAFT_05436 0.73 +0.8 14,468 - H281DRAFT_05436 0.75 +0.6 14,473 - H281DRAFT_05436 0.76 -0.1
Or see this region's nucleotide sequence