Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05298 and H281DRAFT_05299 are separated by 19 nucleotides H281DRAFT_05299 and H281DRAFT_05300 are separated by 90 nucleotides H281DRAFT_05300 and H281DRAFT_05301 are separated by 226 nucleotides
H281DRAFT_05298: H281DRAFT_05298 - amino acid ABC transporter membrane protein 2, PAAT family, at 41,066 to 41,728
_05298
H281DRAFT_05299: H281DRAFT_05299 - amino acid ABC transporter membrane protein 1, PAAT family, at 41,748 to 42,398
_05299
H281DRAFT_05300: H281DRAFT_05300 - amino acid ABC transporter substrate-binding protein, PAAT family, at 42,489 to 43,316
_05300
H281DRAFT_05301: H281DRAFT_05301 - Isopenicillin N synthase, at 43,543 to 44,517
_05301
Position (kb) 42
43
44 Strain fitness (log2 ratio) -2
-1
0
1
2 at 41.553 kb on + strand, within H281DRAFT_05298 at 41.728 kb on + strand at 41.729 kb on - strand at 41.816 kb on - strand, within H281DRAFT_05299 at 41.854 kb on + strand at 41.854 kb on + strand, within H281DRAFT_05299 at 41.855 kb on - strand, within H281DRAFT_05299 at 42.046 kb on - strand, within H281DRAFT_05299 at 42.046 kb on - strand, within H281DRAFT_05299 at 42.046 kb on - strand, within H281DRAFT_05299 at 42.097 kb on + strand, within H281DRAFT_05299 at 42.097 kb on + strand, within H281DRAFT_05299 at 42.098 kb on - strand, within H281DRAFT_05299 at 42.176 kb on - strand, within H281DRAFT_05299 at 42.229 kb on + strand, within H281DRAFT_05299 at 42.468 kb on + strand at 42.719 kb on - strand, within H281DRAFT_05300 at 42.839 kb on - strand, within H281DRAFT_05300 at 42.839 kb on - strand, within H281DRAFT_05300 at 42.976 kb on + strand, within H281DRAFT_05300 at 42.977 kb on - strand, within H281DRAFT_05300 at 43.382 kb on - strand at 43.383 kb on + strand at 43.383 kb on + strand at 43.384 kb on - strand at 43.385 kb on + strand at 43.439 kb on - strand at 43.484 kb on + strand at 43.484 kb on + strand at 43.660 kb on + strand, within H281DRAFT_05301 at 43.660 kb on + strand, within H281DRAFT_05301 at 43.961 kb on - strand, within H281DRAFT_05301 at 43.963 kb on + strand, within H281DRAFT_05301 at 44.215 kb on - strand, within H281DRAFT_05301 at 44.215 kb on - strand, within H281DRAFT_05301
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 41,553 + H281DRAFT_05298 0.73 -0.5 41,728 + +2.3 41,729 - +0.1 41,816 - H281DRAFT_05299 0.10 +0.3 41,854 + -1.9 41,854 + H281DRAFT_05299 0.16 -0.2 41,855 - H281DRAFT_05299 0.16 -0.0 42,046 - H281DRAFT_05299 0.46 -0.2 42,046 - H281DRAFT_05299 0.46 +0.7 42,046 - H281DRAFT_05299 0.46 +1.1 42,097 + H281DRAFT_05299 0.54 +0.7 42,097 + H281DRAFT_05299 0.54 +0.2 42,098 - H281DRAFT_05299 0.54 -0.0 42,176 - H281DRAFT_05299 0.66 +0.7 42,229 + H281DRAFT_05299 0.74 +1.6 42,468 + +1.2 42,719 - H281DRAFT_05300 0.28 -0.4 42,839 - H281DRAFT_05300 0.42 +0.7 42,839 - H281DRAFT_05300 0.42 -0.2 42,976 + H281DRAFT_05300 0.59 +0.5 42,977 - H281DRAFT_05300 0.59 -1.4 43,382 - -0.3 43,383 + -0.1 43,383 + -0.4 43,384 - +0.0 43,385 + -0.1 43,439 - +0.6 43,484 + -0.1 43,484 + -0.1 43,660 + H281DRAFT_05301 0.12 -0.2 43,660 + H281DRAFT_05301 0.12 +1.1 43,961 - H281DRAFT_05301 0.43 -1.1 43,963 + H281DRAFT_05301 0.43 +0.4 44,215 - H281DRAFT_05301 0.69 +1.4 44,215 - H281DRAFT_05301 0.69 +0.3
Or see this region's nucleotide sequence