Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_05057 and H281DRAFT_05058 are separated by 444 nucleotides H281DRAFT_05058 and H281DRAFT_05059 are separated by 137 nucleotides
H281DRAFT_05057: H281DRAFT_05057 - DNA-binding transcriptional regulator, LysR family, at 248,296 to 249,195
_05057
H281DRAFT_05058: H281DRAFT_05058 - Predicted secreted hydrolase, at 249,640 to 249,840
_05058
H281DRAFT_05059: H281DRAFT_05059 - 5S rRNA. Bacterial TSU, at 249,978 to 250,077
_05059
Position (kb) 249
250 Strain fitness (log2 ratio) -1
0
1
2 at 248.855 kb on + strand, within H281DRAFT_05057 at 248.855 kb on + strand, within H281DRAFT_05057 at 248.856 kb on - strand, within H281DRAFT_05057 at 248.963 kb on + strand, within H281DRAFT_05057 at 248.977 kb on - strand, within H281DRAFT_05057 at 249.035 kb on - strand, within H281DRAFT_05057 at 249.035 kb on - strand, within H281DRAFT_05057 at 249.107 kb on + strand at 249.108 kb on - strand at 249.108 kb on - strand at 249.116 kb on - strand at 249.194 kb on - strand at 249.194 kb on - strand at 249.322 kb on + strand at 249.322 kb on + strand at 249.323 kb on - strand at 249.340 kb on - strand at 249.406 kb on + strand at 249.407 kb on - strand at 249.407 kb on - strand at 249.547 kb on + strand at 249.549 kb on + strand at 249.549 kb on + strand at 249.550 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 248,855 + H281DRAFT_05057 0.62 +2.3 248,855 + H281DRAFT_05057 0.62 +0.5 248,856 - H281DRAFT_05057 0.62 +0.7 248,963 + H281DRAFT_05057 0.74 +0.7 248,977 - H281DRAFT_05057 0.76 +0.5 249,035 - H281DRAFT_05057 0.82 -0.0 249,035 - H281DRAFT_05057 0.82 -1.0 249,107 + +1.5 249,108 - +0.5 249,108 - -0.0 249,116 - +0.8 249,194 - +0.1 249,194 - -0.4 249,322 + +0.1 249,322 + +0.3 249,323 - -0.5 249,340 - -0.8 249,406 + -0.7 249,407 - -0.5 249,407 - -1.3 249,547 + -0.1 249,549 + +0.7 249,549 + -0.7 249,550 - -0.3
Or see this region's nucleotide sequence