Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04998 and H281DRAFT_04999 overlap by 4 nucleotides H281DRAFT_04999 and H281DRAFT_05000 overlap by 4 nucleotides H281DRAFT_05000 and H281DRAFT_05001 overlap by 4 nucleotides H281DRAFT_05001 and H281DRAFT_05002 overlap by 16 nucleotides
H281DRAFT_04998: H281DRAFT_04998 - type III secretion protein T, at 184,543 to 185,394
_04998
H281DRAFT_04999: H281DRAFT_04999 - type III secretion protein HrpB7, at 185,391 to 185,885
_04999
H281DRAFT_05000: H281DRAFT_05000 - ATP synthase in type III secretion protein N, at 185,882 to 187,276
_05000
H281DRAFT_05001: H281DRAFT_05001 - type III secretion protein L, at 187,273 to 188,052
_05001
H281DRAFT_05002: H281DRAFT_05002 - type III secretion protein (HrpB4), at 188,037 to 188,852
_05002
Position (kb)
185
186
187
188 Strain fitness (log2 ratio)
-2
-1
0
1 at 184.932 kb on - strand, within H281DRAFT_04998 at 185.634 kb on - strand, within H281DRAFT_04999 at 185.711 kb on - strand, within H281DRAFT_04999 at 185.886 kb on - strand at 185.943 kb on + strand at 186.031 kb on - strand, within H281DRAFT_05000 at 186.031 kb on - strand, within H281DRAFT_05000 at 186.501 kb on + strand, within H281DRAFT_05000 at 187.568 kb on + strand, within H281DRAFT_05001 at 187.569 kb on - strand, within H281DRAFT_05001 at 188.249 kb on + strand, within H281DRAFT_05002
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 184,932 - H281DRAFT_04998 0.46 +0.1 185,634 - H281DRAFT_04999 0.49 -1.1 185,711 - H281DRAFT_04999 0.65 +0.9 185,886 - +0.3 185,943 + +0.1 186,031 - H281DRAFT_05000 0.11 +0.7 186,031 - H281DRAFT_05000 0.11 +0.6 186,501 + H281DRAFT_05000 0.44 +0.2 187,568 + H281DRAFT_05001 0.38 -2.4 187,569 - H281DRAFT_05001 0.38 -1.8 188,249 + H281DRAFT_05002 0.26 -0.1
Or see this region's nucleotide sequence