Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04821 and H281DRAFT_04822 are separated by 396 nucleotides H281DRAFT_04822 and H281DRAFT_04823 are separated by 10 nucleotides H281DRAFT_04823 and H281DRAFT_04824 overlap by 16 nucleotides
H281DRAFT_04821: H281DRAFT_04821 - Nucleoside-diphosphate-sugar epimerase, at 255,907 to 256,818
_04821
H281DRAFT_04822: H281DRAFT_04822 - dTDP-glucose 4,6-dehydratase, at 257,215 to 258,276
_04822
H281DRAFT_04823: H281DRAFT_04823 - Glucose-1-phosphate thymidylyltransferase, at 258,287 to 259,180
_04823
H281DRAFT_04824: H281DRAFT_04824 - dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), at 259,165 to 259,716
_04824
Position (kb) 257
258
259 Strain fitness (log2 ratio) -2
-1
0
1
2 at 256.239 kb on + strand, within H281DRAFT_04821 at 256.239 kb on + strand, within H281DRAFT_04821 at 256.240 kb on - strand, within H281DRAFT_04821 at 256.279 kb on + strand, within H281DRAFT_04821 at 256.279 kb on + strand, within H281DRAFT_04821 at 256.279 kb on + strand, within H281DRAFT_04821 at 256.279 kb on + strand, within H281DRAFT_04821 at 256.280 kb on - strand, within H281DRAFT_04821 at 256.280 kb on - strand, within H281DRAFT_04821 at 256.339 kb on + strand, within H281DRAFT_04821 at 256.339 kb on + strand, within H281DRAFT_04821 at 256.339 kb on + strand, within H281DRAFT_04821 at 256.340 kb on - strand, within H281DRAFT_04821 at 256.423 kb on + strand, within H281DRAFT_04821 at 256.424 kb on - strand, within H281DRAFT_04821 at 256.424 kb on - strand, within H281DRAFT_04821 at 256.424 kb on - strand, within H281DRAFT_04821 at 256.424 kb on - strand, within H281DRAFT_04821 at 256.497 kb on + strand, within H281DRAFT_04821 at 256.513 kb on - strand, within H281DRAFT_04821 at 256.547 kb on - strand, within H281DRAFT_04821 at 256.547 kb on - strand, within H281DRAFT_04821 at 256.562 kb on - strand, within H281DRAFT_04821 at 256.562 kb on - strand, within H281DRAFT_04821 at 256.626 kb on + strand, within H281DRAFT_04821 at 256.626 kb on + strand, within H281DRAFT_04821 at 256.627 kb on - strand, within H281DRAFT_04821 at 256.627 kb on - strand, within H281DRAFT_04821 at 256.627 kb on - strand, within H281DRAFT_04821 at 256.627 kb on - strand, within H281DRAFT_04821 at 256.776 kb on + strand at 256.792 kb on - strand at 256.913 kb on - strand at 256.931 kb on + strand at 256.931 kb on + strand at 256.932 kb on - strand at 256.933 kb on + strand at 256.933 kb on + strand at 256.934 kb on - strand at 256.934 kb on - strand at 256.934 kb on - strand at 256.934 kb on - strand at 256.940 kb on + strand at 256.941 kb on - strand at 256.941 kb on - strand at 256.942 kb on + strand at 256.942 kb on + strand at 256.943 kb on - strand at 256.943 kb on - strand at 256.946 kb on + strand at 256.946 kb on + strand at 257.038 kb on + strand at 257.047 kb on - strand at 257.071 kb on - strand at 257.071 kb on - strand at 257.085 kb on + strand at 258.259 kb on + strand at 258.259 kb on + strand at 259.150 kb on + strand at 259.150 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 256,239 + H281DRAFT_04821 0.36 -2.1 256,239 + H281DRAFT_04821 0.36 +0.1 256,240 - H281DRAFT_04821 0.37 +1.0 256,279 + H281DRAFT_04821 0.41 +0.5 256,279 + H281DRAFT_04821 0.41 -0.1 256,279 + H281DRAFT_04821 0.41 -0.8 256,279 + H281DRAFT_04821 0.41 +0.4 256,280 - H281DRAFT_04821 0.41 -0.5 256,280 - H281DRAFT_04821 0.41 +0.6 256,339 + H281DRAFT_04821 0.47 -0.9 256,339 + H281DRAFT_04821 0.47 -0.8 256,339 + H281DRAFT_04821 0.47 +0.2 256,340 - H281DRAFT_04821 0.47 +1.5 256,423 + H281DRAFT_04821 0.57 +0.9 256,424 - H281DRAFT_04821 0.57 +0.2 256,424 - H281DRAFT_04821 0.57 +0.2 256,424 - H281DRAFT_04821 0.57 -0.7 256,424 - H281DRAFT_04821 0.57 +0.9 256,497 + H281DRAFT_04821 0.65 -0.1 256,513 - H281DRAFT_04821 0.66 -0.5 256,547 - H281DRAFT_04821 0.70 -1.5 256,547 - H281DRAFT_04821 0.70 +0.9 256,562 - H281DRAFT_04821 0.72 -0.9 256,562 - H281DRAFT_04821 0.72 +0.4 256,626 + H281DRAFT_04821 0.79 +0.4 256,626 + H281DRAFT_04821 0.79 +0.4 256,627 - H281DRAFT_04821 0.79 +0.2 256,627 - H281DRAFT_04821 0.79 +0.9 256,627 - H281DRAFT_04821 0.79 +0.4 256,627 - H281DRAFT_04821 0.79 +0.9 256,776 + +1.8 256,792 - +1.7 256,913 - +0.8 256,931 + -0.8 256,931 + -0.1 256,932 - +0.7 256,933 + -0.4 256,933 + +0.0 256,934 - +0.7 256,934 - +0.8 256,934 - +1.0 256,934 - +1.4 256,940 + +0.0 256,941 - +0.5 256,941 - +0.8 256,942 + -0.0 256,942 + -0.1 256,943 - +1.3 256,943 - +1.4 256,946 + -0.4 256,946 + -2.3 257,038 + -0.2 257,047 - +1.3 257,071 - +0.8 257,071 - +0.8 257,085 + -1.2 258,259 + +1.1 258,259 + +1.2 259,150 + +0.8 259,150 + +0.9
Or see this region's nucleotide sequence