Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04553 and H281DRAFT_04554 are separated by 134 nucleotides H281DRAFT_04554 and H281DRAFT_04555 are separated by 24 nucleotides H281DRAFT_04555 and H281DRAFT_04556 are separated by 468 nucleotides
H281DRAFT_04553: H281DRAFT_04553 - ketol-acid reductoisomerase, at 284,958 to 285,974
_04553
H281DRAFT_04554: H281DRAFT_04554 - phosphatidylserine decarboxylase, at 286,109 to 286,747
_04554
H281DRAFT_04555: H281DRAFT_04555 - CDP-diacylglycerol--serine O-phosphatidyltransferase, at 286,772 to 287,641
_04555
H281DRAFT_04556: H281DRAFT_04556 - 2-isopropylmalate synthase, at 288,110 to 289,657
_04556
Position (kb) 286
287
288 Strain fitness (log2 ratio) -1
0
1
2
3 at 285.972 kb on + strand at 285.972 kb on + strand at 285.972 kb on + strand at 285.973 kb on - strand at 285.973 kb on - strand at 285.973 kb on - strand at 286.087 kb on + strand at 287.639 kb on + strand at 287.639 kb on + strand at 287.640 kb on - strand at 287.661 kb on - strand at 287.661 kb on - strand at 287.807 kb on - strand at 287.808 kb on + strand at 287.809 kb on - strand at 287.809 kb on - strand at 287.851 kb on + strand at 287.925 kb on + strand at 287.926 kb on - strand at 288.407 kb on - strand, within H281DRAFT_04556
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 285,972 + -0.1 285,972 + -0.0 285,972 + +0.4 285,973 - -0.0 285,973 - +0.6 285,973 - +2.8 286,087 + -0.4 287,639 + -0.1 287,639 + -0.1 287,640 - +0.3 287,661 - +1.0 287,661 - -0.3 287,807 - +1.3 287,808 + +0.7 287,809 - -0.5 287,809 - +0.3 287,851 + +0.0 287,925 + -1.3 287,926 - -0.9 288,407 - H281DRAFT_04556 0.19 -0.4
Or see this region's nucleotide sequence