Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04442 and H281DRAFT_04443 are separated by 81 nucleotides H281DRAFT_04443 and H281DRAFT_04444 are separated by 40 nucleotides H281DRAFT_04444 and H281DRAFT_04445 are separated by 345 nucleotides
H281DRAFT_04442: H281DRAFT_04442 - gamma-glutamyltransferase 1 (EC:2.3.2.2). Threonine peptidase. MEROPS family T03, at 159,409 to 161,208
_04442
H281DRAFT_04443: H281DRAFT_04443 - hypothetical protein, at 161,290 to 161,850
_04443
H281DRAFT_04444: H281DRAFT_04444 - Crotonobetainyl-CoA:carnitine CoA-transferase CaiB, at 161,891 to 163,111
_04444
H281DRAFT_04445: H281DRAFT_04445 - alanyl-tRNA synthetase, at 163,457 to 166,081
_04445
Position (kb) 161
162
163
164 Strain fitness (log2 ratio) -1
0
1
2 at 161.033 kb on - strand at 161.044 kb on + strand at 161.044 kb on + strand at 161.092 kb on + strand at 161.092 kb on + strand at 161.093 kb on - strand at 161.202 kb on - strand at 161.265 kb on + strand at 161.277 kb on + strand at 161.313 kb on - strand at 161.536 kb on + strand, within H281DRAFT_04443 at 161.536 kb on + strand, within H281DRAFT_04443 at 161.685 kb on + strand, within H281DRAFT_04443 at 161.775 kb on + strand, within H281DRAFT_04443 at 161.775 kb on + strand, within H281DRAFT_04443 at 161.775 kb on + strand, within H281DRAFT_04443 at 161.775 kb on + strand, within H281DRAFT_04443 at 161.776 kb on - strand, within H281DRAFT_04443 at 161.776 kb on - strand, within H281DRAFT_04443 at 161.776 kb on - strand, within H281DRAFT_04443 at 161.776 kb on - strand, within H281DRAFT_04443 at 161.969 kb on - strand at 162.022 kb on + strand, within H281DRAFT_04444 at 162.496 kb on + strand, within H281DRAFT_04444 at 162.497 kb on - strand, within H281DRAFT_04444 at 162.498 kb on + strand, within H281DRAFT_04444 at 162.499 kb on - strand, within H281DRAFT_04444 at 162.499 kb on - strand, within H281DRAFT_04444 at 162.499 kb on - strand, within H281DRAFT_04444 at 162.558 kb on + strand, within H281DRAFT_04444 at 162.558 kb on + strand, within H281DRAFT_04444 at 162.558 kb on + strand, within H281DRAFT_04444 at 162.640 kb on + strand, within H281DRAFT_04444 at 162.640 kb on + strand, within H281DRAFT_04444 at 162.733 kb on - strand, within H281DRAFT_04444 at 162.804 kb on + strand, within H281DRAFT_04444 at 162.804 kb on + strand, within H281DRAFT_04444 at 162.804 kb on + strand, within H281DRAFT_04444 at 162.804 kb on + strand, within H281DRAFT_04444 at 162.804 kb on + strand, within H281DRAFT_04444 at 162.805 kb on - strand, within H281DRAFT_04444 at 162.915 kb on + strand, within H281DRAFT_04444 at 162.918 kb on + strand at 162.918 kb on + strand, within H281DRAFT_04444 at 163.128 kb on + strand at 163.128 kb on + strand at 163.129 kb on - strand at 163.133 kb on - strand at 163.133 kb on - strand at 163.133 kb on - strand at 163.133 kb on - strand at 163.133 kb on - strand at 163.159 kb on + strand at 163.159 kb on + strand at 163.159 kb on + strand at 163.160 kb on - strand at 163.160 kb on - strand at 163.160 kb on - strand at 163.160 kb on - strand at 163.365 kb on + strand at 163.367 kb on + strand at 163.367 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 161,033 - +0.2 161,044 + -0.8 161,044 + -0.1 161,092 + +0.1 161,092 + -0.8 161,093 - -0.5 161,202 - +0.1 161,265 + +0.6 161,277 + -0.2 161,313 - -0.1 161,536 + H281DRAFT_04443 0.44 +0.3 161,536 + H281DRAFT_04443 0.44 +0.4 161,685 + H281DRAFT_04443 0.70 +0.0 161,775 + H281DRAFT_04443 0.86 +1.8 161,775 + H281DRAFT_04443 0.86 -0.9 161,775 + H281DRAFT_04443 0.86 +2.6 161,775 + H281DRAFT_04443 0.86 +0.2 161,776 - H281DRAFT_04443 0.87 +1.2 161,776 - H281DRAFT_04443 0.87 +0.3 161,776 - H281DRAFT_04443 0.87 -0.1 161,776 - H281DRAFT_04443 0.87 -0.2 161,969 - +0.1 162,022 + H281DRAFT_04444 0.11 -0.7 162,496 + H281DRAFT_04444 0.50 +0.5 162,497 - H281DRAFT_04444 0.50 +0.5 162,498 + H281DRAFT_04444 0.50 +0.3 162,499 - H281DRAFT_04444 0.50 -0.1 162,499 - H281DRAFT_04444 0.50 +0.4 162,499 - H281DRAFT_04444 0.50 +0.1 162,558 + H281DRAFT_04444 0.55 +0.4 162,558 + H281DRAFT_04444 0.55 +0.7 162,558 + H281DRAFT_04444 0.55 +0.4 162,640 + H281DRAFT_04444 0.61 +0.5 162,640 + H281DRAFT_04444 0.61 -0.0 162,733 - H281DRAFT_04444 0.69 +0.6 162,804 + H281DRAFT_04444 0.75 +2.5 162,804 + H281DRAFT_04444 0.75 -0.4 162,804 + H281DRAFT_04444 0.75 -0.2 162,804 + H281DRAFT_04444 0.75 +0.4 162,804 + H281DRAFT_04444 0.75 +0.0 162,805 - H281DRAFT_04444 0.75 +0.2 162,915 + H281DRAFT_04444 0.84 -0.2 162,918 + +1.8 162,918 + H281DRAFT_04444 0.84 -0.7 163,128 + -0.8 163,128 + -0.3 163,129 - -0.2 163,133 - -0.1 163,133 - -0.5 163,133 - +0.2 163,133 - +0.8 163,133 - -0.1 163,159 + +2.0 163,159 + +0.1 163,159 + -0.7 163,160 - +0.9 163,160 - -0.0 163,160 - +0.9 163,160 - +0.6 163,365 + +0.9 163,367 + +0.8 163,367 + -1.2
Or see this region's nucleotide sequence