Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04429 and H281DRAFT_04430 overlap by 115 nucleotides H281DRAFT_04430 and H281DRAFT_04431 are separated by 195 nucleotides H281DRAFT_04431 and H281DRAFT_04432 are separated by 175 nucleotides
H281DRAFT_04429: H281DRAFT_04429 - hypothetical protein, at 147,454 to 147,714
_04429
H281DRAFT_04430: H281DRAFT_04430 - hypothetical protein, at 147,600 to 147,926
_04430
H281DRAFT_04431: H281DRAFT_04431 - Membrane-bound metallopeptidase, at 148,122 to 148,838
_04431
H281DRAFT_04432: H281DRAFT_04432 - Galactose mutarotase, at 149,014 to 149,922
_04432
Position (kb) 148
149 Strain fitness (log2 ratio) -2
-1
0
1
2 at 147.406 kb on + strand at 147.406 kb on + strand at 147.426 kb on + strand at 147.434 kb on + strand at 147.448 kb on + strand at 147.449 kb on - strand at 147.449 kb on - strand at 147.470 kb on + strand at 147.470 kb on + strand at 147.471 kb on - strand at 147.601 kb on + strand at 147.609 kb on - strand at 147.609 kb on - strand at 147.718 kb on + strand, within H281DRAFT_04430 at 147.719 kb on - strand, within H281DRAFT_04430 at 147.719 kb on - strand, within H281DRAFT_04430 at 147.824 kb on - strand, within H281DRAFT_04430 at 147.824 kb on - strand, within H281DRAFT_04430 at 147.939 kb on + strand at 147.939 kb on + strand at 147.992 kb on + strand at 147.992 kb on + strand at 147.992 kb on + strand at 147.992 kb on + strand at 147.994 kb on + strand at 147.995 kb on - strand at 148.024 kb on + strand at 148.100 kb on + strand at 148.100 kb on + strand at 148.101 kb on - strand at 148.123 kb on + strand at 148.297 kb on + strand, within H281DRAFT_04431 at 148.623 kb on + strand, within H281DRAFT_04431 at 148.624 kb on - strand, within H281DRAFT_04431 at 148.624 kb on - strand, within H281DRAFT_04431 at 148.700 kb on + strand, within H281DRAFT_04431 at 148.700 kb on + strand, within H281DRAFT_04431 at 148.700 kb on + strand at 148.818 kb on - strand at 148.888 kb on - strand at 148.891 kb on + strand at 148.891 kb on + strand at 148.891 kb on + strand at 148.892 kb on - strand at 148.892 kb on - strand at 148.892 kb on - strand at 149.016 kb on - strand at 149.016 kb on - strand at 149.094 kb on + strand at 149.135 kb on + strand, within H281DRAFT_04432 at 149.135 kb on + strand, within H281DRAFT_04432 at 149.135 kb on + strand, within H281DRAFT_04432 at 149.136 kb on - strand at 149.136 kb on - strand, within H281DRAFT_04432 at 149.248 kb on + strand, within H281DRAFT_04432 at 149.248 kb on + strand, within H281DRAFT_04432 at 149.249 kb on - strand at 149.249 kb on - strand, within H281DRAFT_04432 at 149.249 kb on - strand, within H281DRAFT_04432 at 149.316 kb on - strand, within H281DRAFT_04432 at 149.432 kb on + strand, within H281DRAFT_04432 at 149.432 kb on + strand, within H281DRAFT_04432 at 149.433 kb on - strand, within H281DRAFT_04432 at 149.453 kb on + strand, within H281DRAFT_04432 at 149.454 kb on - strand, within H281DRAFT_04432 at 149.454 kb on - strand, within H281DRAFT_04432
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 147,406 + +0.7 147,406 + +0.6 147,426 + +1.3 147,434 + +0.8 147,448 + -0.3 147,449 - -0.8 147,449 - -0.1 147,470 + -1.5 147,470 + -0.7 147,471 - +1.3 147,601 + +2.4 147,609 - -1.0 147,609 - +0.3 147,718 + H281DRAFT_04430 0.36 +0.7 147,719 - H281DRAFT_04430 0.36 +1.1 147,719 - H281DRAFT_04430 0.36 -0.0 147,824 - H281DRAFT_04430 0.69 -1.1 147,824 - H281DRAFT_04430 0.69 +0.1 147,939 + -0.0 147,939 + -0.6 147,992 + +0.2 147,992 + +0.3 147,992 + +1.8 147,992 + +0.5 147,994 + -0.8 147,995 - +0.3 148,024 + -0.6 148,100 + +0.3 148,100 + -0.6 148,101 - +0.4 148,123 + -0.8 148,297 + H281DRAFT_04431 0.24 +0.0 148,623 + H281DRAFT_04431 0.70 -1.1 148,624 - H281DRAFT_04431 0.70 -2.2 148,624 - H281DRAFT_04431 0.70 -0.5 148,700 + H281DRAFT_04431 0.81 +1.3 148,700 + H281DRAFT_04431 0.81 -0.4 148,700 + +1.1 148,818 - -0.6 148,888 - +1.8 148,891 + -1.5 148,891 + +0.4 148,891 + +0.0 148,892 - +0.5 148,892 - -0.9 148,892 - -0.4 149,016 - +0.0 149,016 - -0.8 149,094 + -0.1 149,135 + H281DRAFT_04432 0.13 +0.4 149,135 + H281DRAFT_04432 0.13 -0.1 149,135 + H281DRAFT_04432 0.13 -2.4 149,136 - -0.2 149,136 - H281DRAFT_04432 0.13 +1.8 149,248 + H281DRAFT_04432 0.26 -2.5 149,248 + H281DRAFT_04432 0.26 +0.6 149,249 - +1.8 149,249 - H281DRAFT_04432 0.26 +0.6 149,249 - H281DRAFT_04432 0.26 +1.5 149,316 - H281DRAFT_04432 0.33 -0.2 149,432 + H281DRAFT_04432 0.46 -0.8 149,432 + H281DRAFT_04432 0.46 +0.5 149,433 - H281DRAFT_04432 0.46 +0.6 149,453 + H281DRAFT_04432 0.48 -0.1 149,454 - H281DRAFT_04432 0.48 +0.7 149,454 - H281DRAFT_04432 0.48 +0.4
Or see this region's nucleotide sequence