Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04074 and H281DRAFT_04075 overlap by 4 nucleotides H281DRAFT_04075 and H281DRAFT_04076 are separated by 158 nucleotides
H281DRAFT_04074: H281DRAFT_04074 - 16S rRNA (cytosine967-C5)-methyltransferase, at 176,101 to 177,360
_04074
H281DRAFT_04075: H281DRAFT_04075 - formyltetrahydrofolate-dependent phosphoribosylglycinamide formyltransferase, at 177,357 to 177,968
_04075
H281DRAFT_04076: H281DRAFT_04076 - FMN adenylyltransferase /riboflavin kinase, at 178,127 to 179,122
_04076
Position (kb) 177
178 Strain fitness (log2 ratio) -1
0
1
2
3 at 176.606 kb on + strand, within H281DRAFT_04074 at 176.606 kb on + strand, within H281DRAFT_04074 at 176.607 kb on - strand, within H281DRAFT_04074 at 176.607 kb on - strand, within H281DRAFT_04074 at 176.607 kb on - strand, within H281DRAFT_04074 at 176.866 kb on + strand, within H281DRAFT_04074 at 176.866 kb on + strand, within H281DRAFT_04074 at 176.866 kb on + strand, within H281DRAFT_04074 at 176.866 kb on + strand, within H281DRAFT_04074 at 176.867 kb on - strand, within H281DRAFT_04074 at 176.921 kb on + strand, within H281DRAFT_04074 at 176.984 kb on + strand, within H281DRAFT_04074 at 176.985 kb on - strand, within H281DRAFT_04074 at 176.985 kb on - strand, within H281DRAFT_04074 at 176.985 kb on - strand, within H281DRAFT_04074 at 176.985 kb on - strand, within H281DRAFT_04074 at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.352 kb on + strand at 177.353 kb on - strand at 177.413 kb on + strand at 177.414 kb on - strand at 177.414 kb on - strand at 177.479 kb on - strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.482 kb on + strand, within H281DRAFT_04075 at 177.483 kb on - strand, within H281DRAFT_04075 at 177.483 kb on - strand, within H281DRAFT_04075 at 177.483 kb on - strand, within H281DRAFT_04075 at 177.744 kb on - strand, within H281DRAFT_04075 at 177.959 kb on + strand at 177.959 kb on + strand at 177.960 kb on - strand at 178.076 kb on + strand at 178.076 kb on + strand at 178.077 kb on - strand at 178.078 kb on + strand at 178.078 kb on + strand at 178.078 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 176,606 + H281DRAFT_04074 0.40 -1.5 176,606 + H281DRAFT_04074 0.40 +0.9 176,607 - H281DRAFT_04074 0.40 -0.6 176,607 - H281DRAFT_04074 0.40 +0.2 176,607 - H281DRAFT_04074 0.40 +0.7 176,866 + H281DRAFT_04074 0.61 +0.2 176,866 + H281DRAFT_04074 0.61 +0.4 176,866 + H281DRAFT_04074 0.61 +0.1 176,866 + H281DRAFT_04074 0.61 -0.4 176,867 - H281DRAFT_04074 0.61 -0.3 176,921 + H281DRAFT_04074 0.65 +2.9 176,984 + H281DRAFT_04074 0.70 -1.6 176,985 - H281DRAFT_04074 0.70 -1.0 176,985 - H281DRAFT_04074 0.70 +0.5 176,985 - H281DRAFT_04074 0.70 -0.6 176,985 - H281DRAFT_04074 0.70 +0.3 177,352 + -0.2 177,352 + +1.9 177,352 + -0.4 177,352 + +0.4 177,352 + +0.6 177,352 + +0.7 177,352 + +0.6 177,352 + +0.1 177,352 + -0.1 177,353 - -0.8 177,413 + -0.1 177,414 - -0.4 177,414 - +0.1 177,479 - H281DRAFT_04075 0.20 +0.9 177,482 + H281DRAFT_04075 0.20 -0.1 177,482 + H281DRAFT_04075 0.20 -0.5 177,482 + H281DRAFT_04075 0.20 +0.8 177,482 + H281DRAFT_04075 0.20 -0.1 177,482 + H281DRAFT_04075 0.20 +0.5 177,482 + H281DRAFT_04075 0.20 +0.1 177,482 + H281DRAFT_04075 0.20 +1.2 177,482 + H281DRAFT_04075 0.20 -1.2 177,482 + H281DRAFT_04075 0.20 +0.2 177,482 + H281DRAFT_04075 0.20 +0.4 177,483 - H281DRAFT_04075 0.21 +1.3 177,483 - H281DRAFT_04075 0.21 +0.5 177,483 - H281DRAFT_04075 0.21 -0.9 177,744 - H281DRAFT_04075 0.63 -0.3 177,959 + +0.4 177,959 + +1.8 177,960 - -1.0 178,076 + -0.3 178,076 + -0.3 178,077 - -1.0 178,078 + -1.0 178,078 + +0.0 178,078 + -0.1
Or see this region's nucleotide sequence