Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04043 and H281DRAFT_04044 are separated by 56 nucleotides H281DRAFT_04044 and H281DRAFT_04045 overlap by 1 nucleotides
H281DRAFT_04043: H281DRAFT_04043 - Pimeloyl-ACP methyl ester carboxylesterase, at 142,780 to 143,745
_04043
H281DRAFT_04044: H281DRAFT_04044 - D-aminopeptidase DppA. Metallo peptidase. MEROPS family M55, at 143,802 to 144,635
_04044
H281DRAFT_04045: H281DRAFT_04045 - L-aminopeptidase DmpA. Serine peptidase. MEROPS family S58, at 144,635 to 145,747
_04045
Position (kb) 143
144
145 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 142.974 kb on + strand, within H281DRAFT_04043 at 142.974 kb on + strand, within H281DRAFT_04043 at 142.974 kb on + strand, within H281DRAFT_04043 at 142.974 kb on + strand, within H281DRAFT_04043 at 142.975 kb on - strand, within H281DRAFT_04043 at 142.975 kb on - strand at 142.975 kb on - strand, within H281DRAFT_04043 at 142.975 kb on - strand, within H281DRAFT_04043 at 142.975 kb on - strand, within H281DRAFT_04043 at 143.007 kb on + strand, within H281DRAFT_04043 at 143.308 kb on + strand, within H281DRAFT_04043 at 143.309 kb on - strand, within H281DRAFT_04043 at 143.309 kb on - strand, within H281DRAFT_04043 at 143.433 kb on + strand at 143.437 kb on + strand, within H281DRAFT_04043 at 143.502 kb on - strand, within H281DRAFT_04043 at 143.569 kb on + strand, within H281DRAFT_04043 at 143.709 kb on - strand at 143.746 kb on + strand at 143.746 kb on + strand at 143.746 kb on + strand at 143.747 kb on - strand at 144.203 kb on - strand, within H281DRAFT_04044 at 144.290 kb on + strand, within H281DRAFT_04044 at 144.290 kb on + strand, within H281DRAFT_04044 at 144.290 kb on + strand, within H281DRAFT_04044 at 144.358 kb on + strand, within H281DRAFT_04044 at 144.689 kb on + strand at 144.852 kb on + strand, within H281DRAFT_04045 at 144.852 kb on + strand, within H281DRAFT_04045 at 145.155 kb on + strand, within H281DRAFT_04045 at 145.156 kb on - strand, within H281DRAFT_04045 at 145.278 kb on + strand, within H281DRAFT_04045 at 145.279 kb on - strand, within H281DRAFT_04045 at 145.332 kb on + strand, within H281DRAFT_04045 at 145.450 kb on + strand, within H281DRAFT_04045 at 145.450 kb on + strand, within H281DRAFT_04045 at 145.607 kb on + strand, within H281DRAFT_04045
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 142,974 + H281DRAFT_04043 0.20 -0.3 142,974 + H281DRAFT_04043 0.20 +0.2 142,974 + H281DRAFT_04043 0.20 -0.3 142,974 + H281DRAFT_04043 0.20 +0.2 142,975 - H281DRAFT_04043 0.20 +0.9 142,975 - +2.5 142,975 - H281DRAFT_04043 0.20 +0.0 142,975 - H281DRAFT_04043 0.20 +0.7 142,975 - H281DRAFT_04043 0.20 +0.4 143,007 + H281DRAFT_04043 0.23 +0.3 143,308 + H281DRAFT_04043 0.55 -3.0 143,309 - H281DRAFT_04043 0.55 +2.1 143,309 - H281DRAFT_04043 0.55 -0.9 143,433 + +0.9 143,437 + H281DRAFT_04043 0.68 +0.4 143,502 - H281DRAFT_04043 0.75 +0.6 143,569 + H281DRAFT_04043 0.82 +1.9 143,709 - -0.8 143,746 + -1.7 143,746 + +0.2 143,746 + +0.2 143,747 - +1.6 144,203 - H281DRAFT_04044 0.48 +0.2 144,290 + H281DRAFT_04044 0.59 +0.7 144,290 + H281DRAFT_04044 0.59 -0.4 144,290 + H281DRAFT_04044 0.59 +0.1 144,358 + H281DRAFT_04044 0.67 +0.4 144,689 + +0.5 144,852 + H281DRAFT_04045 0.19 -1.2 144,852 + H281DRAFT_04045 0.19 +0.1 145,155 + H281DRAFT_04045 0.47 -1.8 145,156 - H281DRAFT_04045 0.47 -1.1 145,278 + H281DRAFT_04045 0.58 -0.1 145,279 - H281DRAFT_04045 0.58 +1.4 145,332 + H281DRAFT_04045 0.63 -0.8 145,450 + H281DRAFT_04045 0.73 -0.2 145,450 + H281DRAFT_04045 0.73 -0.2 145,607 + H281DRAFT_04045 0.87 -0.2
Or see this region's nucleotide sequence