Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03852 and H281DRAFT_03853 are separated by 490 nucleotides H281DRAFT_03853 and H281DRAFT_03854 are separated by 14 nucleotides H281DRAFT_03854 and H281DRAFT_03855 overlap by 4 nucleotides
H281DRAFT_03852: H281DRAFT_03852 - Nitroreductase, at 381,466 to 382,167
_03852
H281DRAFT_03853: H281DRAFT_03853 - UTP:GlnB (protein PII) uridylyltransferase, at 382,658 to 383,341
_03853
H281DRAFT_03854: H281DRAFT_03854 - Predicted metal-dependent hydrolase, TIM-barrel fold, at 383,356 to 384,198
_03854
H281DRAFT_03855: H281DRAFT_03855 - Sugar phosphate isomerase/epimerase, at 384,195 to 385,043
_03855
Position (kb) 382
383
384 Strain fitness (log2 ratio) -1
0
1 at 381.938 kb on + strand, within H281DRAFT_03852 at 381.938 kb on + strand, within H281DRAFT_03852 at 381.939 kb on - strand, within H281DRAFT_03852 at 382.049 kb on - strand, within H281DRAFT_03852 at 382.378 kb on + strand at 382.642 kb on + strand at 382.812 kb on - strand at 382.812 kb on - strand, within H281DRAFT_03853 at 383.329 kb on + strand at 383.329 kb on + strand at 383.330 kb on - strand at 383.364 kb on + strand at 383.580 kb on - strand, within H281DRAFT_03854 at 383.723 kb on - strand, within H281DRAFT_03854 at 383.723 kb on - strand, within H281DRAFT_03854 at 384.029 kb on - strand, within H281DRAFT_03854 at 384.029 kb on - strand, within H281DRAFT_03854 at 384.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 381,938 + H281DRAFT_03852 0.67 +0.9 381,938 + H281DRAFT_03852 0.67 -0.9 381,939 - H281DRAFT_03852 0.67 +0.5 382,049 - H281DRAFT_03852 0.83 -1.0 382,378 + +1.0 382,642 + -1.6 382,812 - +1.5 382,812 - H281DRAFT_03853 0.23 +0.6 383,329 + -0.3 383,329 + +0.1 383,330 - -0.3 383,364 + +0.1 383,580 - H281DRAFT_03854 0.27 -0.2 383,723 - H281DRAFT_03854 0.44 +0.3 383,723 - H281DRAFT_03854 0.44 +0.5 384,029 - H281DRAFT_03854 0.80 -1.1 384,029 - H281DRAFT_03854 0.80 -0.2 384,114 - -0.0
Or see this region's nucleotide sequence