Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03323 and H281DRAFT_03324 overlap by 4 nucleotides H281DRAFT_03324 and H281DRAFT_03325 are separated by 57 nucleotides H281DRAFT_03325 and H281DRAFT_03326 are separated by 63 nucleotides H281DRAFT_03326 and H281DRAFT_03327 are separated by 214 nucleotides H281DRAFT_03327 and H281DRAFT_03328 are separated by 153 nucleotides
H281DRAFT_03323: H281DRAFT_03323 - hypothetical protein, at 383,361 to 383,996
_03323
H281DRAFT_03324: H281DRAFT_03324 - Low affinity Fe/Cu permease, at 383,993 to 384,520
_03324
H281DRAFT_03325: H281DRAFT_03325 - putative regulatory protein, FmdB family, at 384,578 to 384,793
_03325
H281DRAFT_03326: H281DRAFT_03326 - PAS domain S-box-containing protein, at 384,857 to 387,025
_03326
H281DRAFT_03327: H281DRAFT_03327 - Protein of unknown function (DUF2795), at 387,240 to 387,461
_03327
H281DRAFT_03328: H281DRAFT_03328 - hypothetical protein, at 387,615 to 388,034
_03328
Position (kb) 384
385
386
387
388 Strain fitness (log2 ratio) -1
0
1 at 383.956 kb on + strand at 383.957 kb on - strand at 383.957 kb on - strand at 384.080 kb on + strand, within H281DRAFT_03324 at 384.102 kb on + strand, within H281DRAFT_03324 at 384.778 kb on - strand at 384.778 kb on - strand at 384.784 kb on - strand at 384.784 kb on - strand at 384.810 kb on - strand at 384.850 kb on - strand at 385.778 kb on + strand, within H281DRAFT_03326 at 386.026 kb on + strand, within H281DRAFT_03326 at 386.063 kb on + strand, within H281DRAFT_03326 at 386.205 kb on + strand, within H281DRAFT_03326 at 386.362 kb on - strand, within H281DRAFT_03326 at 387.129 kb on - strand at 387.129 kb on - strand at 387.138 kb on - strand at 387.539 kb on + strand at 387.540 kb on - strand at 387.788 kb on - strand, within H281DRAFT_03328 at 387.801 kb on + strand, within H281DRAFT_03328 at 387.802 kb on - strand, within H281DRAFT_03328
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 383,956 + -0.0 383,957 - -0.3 383,957 - +0.1 384,080 + H281DRAFT_03324 0.16 +0.6 384,102 + H281DRAFT_03324 0.21 -0.1 384,778 - +0.5 384,778 - +0.0 384,784 - +1.4 384,784 - -0.9 384,810 - +0.4 384,850 - +1.0 385,778 + H281DRAFT_03326 0.42 -0.4 386,026 + H281DRAFT_03326 0.54 +0.1 386,063 + H281DRAFT_03326 0.56 +0.3 386,205 + H281DRAFT_03326 0.62 +0.0 386,362 - H281DRAFT_03326 0.69 -0.5 387,129 - +0.8 387,129 - -0.7 387,138 - -0.0 387,539 + -0.1 387,540 - -0.3 387,788 - H281DRAFT_03328 0.41 -0.2 387,801 + H281DRAFT_03328 0.44 -1.3 387,802 - H281DRAFT_03328 0.45 -0.4
Or see this region's nucleotide sequence