Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03151 and H281DRAFT_03152 are separated by 54 nucleotides H281DRAFT_03152 and H281DRAFT_03153 are separated by 43 nucleotides H281DRAFT_03153 and H281DRAFT_03154 overlap by 4 nucleotides
H281DRAFT_03151: H281DRAFT_03151 - hypothetical protein, at 190,552 to 191,037
_03151
H281DRAFT_03152: H281DRAFT_03152 - hypothetical protein, at 191,092 to 191,673
_03152
H281DRAFT_03153: H281DRAFT_03153 - two component transcriptional regulator, LuxR family, at 191,717 to 192,364
_03153
H281DRAFT_03154: H281DRAFT_03154 - two-component system, NarL family, sensor kinase, at 192,361 to 193,782
_03154
Position (kb) 191
192
193 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 190.840 kb on + strand, within H281DRAFT_03151 at 190.841 kb on - strand, within H281DRAFT_03151 at 191.166 kb on + strand, within H281DRAFT_03152 at 191.272 kb on - strand, within H281DRAFT_03152 at 191.384 kb on + strand, within H281DRAFT_03152 at 191.517 kb on + strand, within H281DRAFT_03152 at 191.619 kb on + strand at 191.619 kb on + strand at 191.620 kb on - strand at 191.818 kb on + strand, within H281DRAFT_03153 at 192.078 kb on + strand, within H281DRAFT_03153 at 192.353 kb on + strand at 193.284 kb on - strand, within H281DRAFT_03154 at 193.341 kb on - strand, within H281DRAFT_03154
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 190,840 + H281DRAFT_03151 0.59 -0.3 190,841 - H281DRAFT_03151 0.59 +0.7 191,166 + H281DRAFT_03152 0.13 -0.2 191,272 - H281DRAFT_03152 0.31 +0.8 191,384 + H281DRAFT_03152 0.50 -0.6 191,517 + H281DRAFT_03152 0.73 -1.7 191,619 + -0.7 191,619 + -0.4 191,620 - -0.9 191,818 + H281DRAFT_03153 0.16 -0.2 192,078 + H281DRAFT_03153 0.56 -0.9 192,353 + +4.7 193,284 - H281DRAFT_03154 0.65 +0.6 193,341 - H281DRAFT_03154 0.69 -1.7
Or see this region's nucleotide sequence