Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03118 and H281DRAFT_03119 overlap by 4 nucleotides H281DRAFT_03119 and H281DRAFT_03120 are separated by 23 nucleotides H281DRAFT_03120 and H281DRAFT_03121 are separated by 27 nucleotides
H281DRAFT_03118: H281DRAFT_03118 - NADPH-dependent 2,4-dienoyl-CoA reductase, sulfur reductase, at 151,204 to 152,637
_03118
H281DRAFT_03119: H281DRAFT_03119 - NADH dehydrogenase/NADH:ubiquinone oxidoreductase 75 kD subunit (chain G), at 152,634 to 153,002
_03119
H281DRAFT_03120: H281DRAFT_03120 - putative spermidine/putrescine transport system permease protein, at 153,026 to 153,820
_03120
H281DRAFT_03121: H281DRAFT_03121 - putative spermidine/putrescine transport system permease protein, at 153,848 to 154,762
_03121
Position (kb) 152
153
154 Strain fitness (log2 ratio) -1
0
1
2 at 152.009 kb on + strand, within H281DRAFT_03118 at 152.230 kb on - strand, within H281DRAFT_03118 at 153.158 kb on - strand, within H281DRAFT_03120 at 153.158 kb on - strand, within H281DRAFT_03120 at 153.160 kb on - strand, within H281DRAFT_03120 at 153.397 kb on + strand, within H281DRAFT_03120 at 153.867 kb on + strand at 153.867 kb on + strand at 153.891 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 152,009 + H281DRAFT_03118 0.56 +2.0 152,230 - H281DRAFT_03118 0.72 -1.0 153,158 - H281DRAFT_03120 0.17 -0.0 153,158 - H281DRAFT_03120 0.17 -0.0 153,160 - H281DRAFT_03120 0.17 -0.0 153,397 + H281DRAFT_03120 0.47 +1.6 153,867 + -0.4 153,867 + -0.2 153,891 + +0.1
Or see this region's nucleotide sequence