Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03086 and H281DRAFT_03087 are separated by 549 nucleotides H281DRAFT_03087 and H281DRAFT_03088 are separated by 349 nucleotides H281DRAFT_03088 and H281DRAFT_03089 are separated by 148 nucleotides
H281DRAFT_03086: H281DRAFT_03086 - protein of unknown function (DUF4410), at 115,642 to 116,409
_03086
H281DRAFT_03087: H281DRAFT_03087 - hypothetical protein, at 116,959 to 117,435
_03087
H281DRAFT_03088: H281DRAFT_03088 - phasin family protein, at 117,785 to 118,222
_03088
H281DRAFT_03089: H281DRAFT_03089 - hypothetical protein, at 118,371 to 119,495
_03089
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-2
-1
0
1 at 116.056 kb on + strand, within H281DRAFT_03086 at 116.056 kb on + strand, within H281DRAFT_03086 at 116.056 kb on + strand, within H281DRAFT_03086 at 116.178 kb on + strand, within H281DRAFT_03086 at 116.252 kb on + strand, within H281DRAFT_03086 at 116.253 kb on - strand, within H281DRAFT_03086 at 116.452 kb on + strand at 116.453 kb on - strand at 116.501 kb on - strand at 116.565 kb on + strand at 116.830 kb on + strand at 116.884 kb on + strand at 116.884 kb on + strand at 116.960 kb on + strand at 117.108 kb on - strand, within H281DRAFT_03087 at 117.108 kb on - strand, within H281DRAFT_03087 at 117.108 kb on - strand, within H281DRAFT_03087 at 117.315 kb on + strand, within H281DRAFT_03087 at 117.769 kb on + strand at 118.137 kb on - strand, within H281DRAFT_03088 at 118.325 kb on - strand at 118.372 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 116,056 + H281DRAFT_03086 0.54 +0.3 116,056 + H281DRAFT_03086 0.54 -0.1 116,056 + H281DRAFT_03086 0.54 -0.1 116,178 + H281DRAFT_03086 0.70 +0.2 116,252 + H281DRAFT_03086 0.79 +0.7 116,253 - H281DRAFT_03086 0.80 -1.8 116,452 + -1.5 116,453 - -0.0 116,501 - +0.1 116,565 + -0.5 116,830 + -1.5 116,884 + -0.4 116,884 + +0.5 116,960 + +0.5 117,108 - H281DRAFT_03087 0.31 +0.6 117,108 - H281DRAFT_03087 0.31 -0.3 117,108 - H281DRAFT_03087 0.31 +0.2 117,315 + H281DRAFT_03087 0.75 +0.2 117,769 + -0.7 118,137 - H281DRAFT_03088 0.80 -1.2 118,325 - +1.5 118,372 + -0.1
Or see this region's nucleotide sequence