Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_03020 and H281DRAFT_03021 are separated by 29 nucleotides H281DRAFT_03021 and H281DRAFT_03022 are separated by 42 nucleotides H281DRAFT_03022 and H281DRAFT_03023 overlap by 19 nucleotides H281DRAFT_03023 and H281DRAFT_03024 are separated by 204 nucleotides
H281DRAFT_03020: H281DRAFT_03020 - uncharacterized protein, YigZ family, at 48,280 to 49,002
_03020
H281DRAFT_03021: H281DRAFT_03021 - Threonine/homoserine/homoserine lactone efflux protein, at 49,032 to 49,658
_03021
H281DRAFT_03022: H281DRAFT_03022 - 2-hydroxychromene-2-carboxylate isomerase, at 49,701 to 50,333
_03022
H281DRAFT_03023: H281DRAFT_03023 - Protein of unknown function (DUF2866), at 50,315 to 50,572
_03023
H281DRAFT_03024: H281DRAFT_03024 - peptidoglycan synthetase FtsI, at 50,777 to 52,513
_03024
Position (kb) 49
50
51 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 48.854 kb on + strand, within H281DRAFT_03020 at 48.854 kb on + strand, within H281DRAFT_03020 at 48.854 kb on + strand, within H281DRAFT_03020 at 48.962 kb on - strand at 48.962 kb on - strand at 48.977 kb on - strand at 49.073 kb on - strand at 49.403 kb on + strand, within H281DRAFT_03021 at 49.563 kb on - strand, within H281DRAFT_03021 at 49.563 kb on - strand, within H281DRAFT_03021 at 49.696 kb on + strand at 49.773 kb on + strand, within H281DRAFT_03022 at 49.773 kb on + strand, within H281DRAFT_03022 at 49.773 kb on + strand, within H281DRAFT_03022 at 49.774 kb on - strand, within H281DRAFT_03022 at 49.776 kb on - strand, within H281DRAFT_03022 at 49.776 kb on - strand, within H281DRAFT_03022 at 49.779 kb on + strand, within H281DRAFT_03022 at 50.390 kb on - strand, within H281DRAFT_03023 at 50.555 kb on + strand at 50.556 kb on - strand at 50.717 kb on + strand at 50.772 kb on + strand at 50.772 kb on + strand at 50.773 kb on - strand at 50.773 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 48,854 + H281DRAFT_03020 0.79 -0.5 48,854 + H281DRAFT_03020 0.79 +0.7 48,854 + H281DRAFT_03020 0.79 -1.1 48,962 - +0.3 48,962 - +0.3 48,977 - -0.3 49,073 - +0.2 49,403 + H281DRAFT_03021 0.59 -0.8 49,563 - H281DRAFT_03021 0.85 +1.4 49,563 - H281DRAFT_03021 0.85 +0.2 49,696 + -0.3 49,773 + H281DRAFT_03022 0.11 -3.6 49,773 + H281DRAFT_03022 0.11 -0.8 49,773 + H281DRAFT_03022 0.11 +0.1 49,774 - H281DRAFT_03022 0.12 -0.3 49,776 - H281DRAFT_03022 0.12 -0.6 49,776 - H281DRAFT_03022 0.12 -1.3 49,779 + H281DRAFT_03022 0.12 -0.6 50,390 - H281DRAFT_03023 0.29 -0.8 50,555 + +0.9 50,556 - -0.2 50,717 + +0.9 50,772 + +0.4 50,772 + -0.5 50,773 - -0.5 50,773 - -0.6
Or see this region's nucleotide sequence