Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02279 and H281DRAFT_02280 are separated by 2 nucleotides H281DRAFT_02280 and H281DRAFT_02281 are separated by 207 nucleotides
H281DRAFT_02279: H281DRAFT_02279 - Protein of unknown function (DUF3987), at 424,817 to 426,265
_02279
H281DRAFT_02280: H281DRAFT_02280 - hypothetical protein, at 426,268 to 426,498
_02280
H281DRAFT_02281: H281DRAFT_02281 - hypothetical protein, at 426,706 to 427,509
_02281
Position (kb) 426
427 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 425.281 kb on - strand, within H281DRAFT_02279 at 425.656 kb on + strand, within H281DRAFT_02279 at 425.658 kb on + strand, within H281DRAFT_02279 at 425.659 kb on - strand, within H281DRAFT_02279 at 425.659 kb on - strand, within H281DRAFT_02279 at 425.659 kb on - strand, within H281DRAFT_02279 at 425.659 kb on - strand, within H281DRAFT_02279 at 425.659 kb on - strand, within H281DRAFT_02279 at 425.868 kb on + strand, within H281DRAFT_02279 at 425.869 kb on - strand, within H281DRAFT_02279 at 425.869 kb on - strand, within H281DRAFT_02279 at 426.189 kb on + strand at 426.189 kb on + strand at 426.190 kb on - strand at 426.252 kb on + strand at 426.253 kb on - strand at 426.270 kb on - strand at 426.270 kb on - strand at 426.399 kb on - strand, within H281DRAFT_02280 at 426.461 kb on + strand, within H281DRAFT_02280 at 426.519 kb on - strand at 426.519 kb on - strand at 426.519 kb on - strand at 426.646 kb on + strand at 426.646 kb on + strand at 426.647 kb on - strand at 426.647 kb on - strand at 426.659 kb on + strand at 426.759 kb on + strand at 426.759 kb on + strand at 426.794 kb on + strand, within H281DRAFT_02281 at 426.796 kb on + strand, within H281DRAFT_02281 at 426.797 kb on - strand, within H281DRAFT_02281 at 426.797 kb on - strand, within H281DRAFT_02281 at 426.906 kb on + strand, within H281DRAFT_02281 at 426.919 kb on + strand, within H281DRAFT_02281 at 426.919 kb on + strand, within H281DRAFT_02281 at 426.920 kb on - strand, within H281DRAFT_02281 at 426.982 kb on + strand, within H281DRAFT_02281 at 426.983 kb on - strand, within H281DRAFT_02281 at 427.046 kb on - strand, within H281DRAFT_02281 at 427.078 kb on + strand, within H281DRAFT_02281 at 427.079 kb on - strand, within H281DRAFT_02281 at 427.079 kb on - strand, within H281DRAFT_02281 at 427.109 kb on + strand, within H281DRAFT_02281 at 427.109 kb on + strand, within H281DRAFT_02281 at 427.109 kb on + strand, within H281DRAFT_02281 at 427.150 kb on + strand, within H281DRAFT_02281 at 427.151 kb on - strand, within H281DRAFT_02281 at 427.212 kb on + strand, within H281DRAFT_02281 at 427.213 kb on - strand, within H281DRAFT_02281 at 427.213 kb on - strand, within H281DRAFT_02281 at 427.266 kb on - strand, within H281DRAFT_02281 at 427.266 kb on - strand, within H281DRAFT_02281 at 427.285 kb on + strand, within H281DRAFT_02281 at 427.286 kb on - strand, within H281DRAFT_02281 at 427.286 kb on - strand, within H281DRAFT_02281 at 427.387 kb on + strand at 427.387 kb on + strand, within H281DRAFT_02281 at 427.388 kb on - strand, within H281DRAFT_02281 at 427.388 kb on - strand, within H281DRAFT_02281 at 427.388 kb on - strand, within H281DRAFT_02281 at 427.453 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 425,281 - H281DRAFT_02279 0.32 +0.0 425,656 + H281DRAFT_02279 0.58 -0.4 425,658 + H281DRAFT_02279 0.58 +1.1 425,659 - H281DRAFT_02279 0.58 -0.3 425,659 - H281DRAFT_02279 0.58 -0.6 425,659 - H281DRAFT_02279 0.58 -0.2 425,659 - H281DRAFT_02279 0.58 -0.4 425,659 - H281DRAFT_02279 0.58 -0.5 425,868 + H281DRAFT_02279 0.73 -0.0 425,869 - H281DRAFT_02279 0.73 +0.2 425,869 - H281DRAFT_02279 0.73 -0.2 426,189 + +0.6 426,189 + +0.6 426,190 - -0.9 426,252 + +0.2 426,253 - -0.2 426,270 - +0.1 426,270 - -0.1 426,399 - H281DRAFT_02280 0.57 +0.5 426,461 + H281DRAFT_02280 0.84 -0.0 426,519 - +1.6 426,519 - -0.4 426,519 - -0.8 426,646 + -0.6 426,646 + +0.4 426,647 - +2.4 426,647 - -0.2 426,659 + -0.3 426,759 + +0.5 426,759 + +0.4 426,794 + H281DRAFT_02281 0.11 +0.1 426,796 + H281DRAFT_02281 0.11 -1.1 426,797 - H281DRAFT_02281 0.11 -0.1 426,797 - H281DRAFT_02281 0.11 +0.2 426,906 + H281DRAFT_02281 0.25 +2.5 426,919 + H281DRAFT_02281 0.26 +0.1 426,919 + H281DRAFT_02281 0.26 -0.1 426,920 - H281DRAFT_02281 0.27 +3.9 426,982 + H281DRAFT_02281 0.34 +0.1 426,983 - H281DRAFT_02281 0.34 -1.4 427,046 - H281DRAFT_02281 0.42 -0.5 427,078 + H281DRAFT_02281 0.46 -0.1 427,079 - H281DRAFT_02281 0.46 -0.1 427,079 - H281DRAFT_02281 0.46 -0.4 427,109 + H281DRAFT_02281 0.50 +0.0 427,109 + H281DRAFT_02281 0.50 -0.4 427,109 + H281DRAFT_02281 0.50 +0.2 427,150 + H281DRAFT_02281 0.55 +0.3 427,151 - H281DRAFT_02281 0.55 +0.8 427,212 + H281DRAFT_02281 0.63 +0.3 427,213 - H281DRAFT_02281 0.63 +1.0 427,213 - H281DRAFT_02281 0.63 -0.2 427,266 - H281DRAFT_02281 0.70 -0.8 427,266 - H281DRAFT_02281 0.70 -0.3 427,285 + H281DRAFT_02281 0.72 +0.8 427,286 - H281DRAFT_02281 0.72 +2.7 427,286 - H281DRAFT_02281 0.72 -0.9 427,387 + -0.6 427,387 + H281DRAFT_02281 0.85 -0.0 427,388 - H281DRAFT_02281 0.85 +0.0 427,388 - H281DRAFT_02281 0.85 +0.2 427,388 - H281DRAFT_02281 0.85 +0.0 427,453 + -0.3
Or see this region's nucleotide sequence