Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_02144 and H281DRAFT_02145 are separated by 281 nucleotides H281DRAFT_02145 and H281DRAFT_02146 are separated by 349 nucleotides
H281DRAFT_02144: H281DRAFT_02144 - hypothetical protein, at 285,631 to 287,049
_02144
H281DRAFT_02145: H281DRAFT_02145 - hypothetical protein, at 287,331 to 287,441
_02145
H281DRAFT_02146: H281DRAFT_02146 - Integrase, at 287,791 to 289,005
_02146
Position (kb) 287
288 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 286.380 kb on + strand, within H281DRAFT_02144 at 286.380 kb on + strand, within H281DRAFT_02144 at 286.380 kb on + strand, within H281DRAFT_02144 at 286.381 kb on - strand, within H281DRAFT_02144 at 286.381 kb on - strand, within H281DRAFT_02144 at 286.684 kb on + strand, within H281DRAFT_02144 at 286.909 kb on + strand at 286.909 kb on + strand at 286.909 kb on + strand at 286.909 kb on + strand at 286.961 kb on + strand at 286.961 kb on + strand at 286.961 kb on + strand at 286.962 kb on - strand at 287.049 kb on + strand at 287.050 kb on - strand at 287.050 kb on - strand at 287.107 kb on + strand at 287.141 kb on + strand at 287.163 kb on + strand at 287.264 kb on - strand at 287.304 kb on + strand at 287.320 kb on - strand at 287.393 kb on - strand, within H281DRAFT_02145 at 287.559 kb on + strand at 287.559 kb on + strand at 287.559 kb on + strand at 287.560 kb on - strand at 287.560 kb on - strand at 287.560 kb on - strand at 287.560 kb on - strand at 287.560 kb on - strand at 287.583 kb on + strand at 287.653 kb on + strand at 287.653 kb on + strand at 287.654 kb on - strand at 287.654 kb on - strand at 287.654 kb on - strand at 287.654 kb on - strand at 287.654 kb on - strand at 287.654 kb on - strand at 287.654 kb on - strand at 287.654 kb on - strand at 287.662 kb on - strand at 287.662 kb on - strand at 287.662 kb on - strand at 287.739 kb on + strand at 287.740 kb on - strand at 287.775 kb on + strand at 287.850 kb on - strand at 287.850 kb on - strand at 287.906 kb on + strand at 287.906 kb on + strand at 287.906 kb on + strand at 287.907 kb on - strand at 287.907 kb on - strand at 287.907 kb on - strand at 287.913 kb on + strand, within H281DRAFT_02146 at 287.914 kb on - strand, within H281DRAFT_02146 at 288.111 kb on + strand, within H281DRAFT_02146 at 288.112 kb on - strand, within H281DRAFT_02146 at 288.184 kb on + strand, within H281DRAFT_02146 at 288.185 kb on - strand, within H281DRAFT_02146 at 288.217 kb on - strand, within H281DRAFT_02146 at 288.217 kb on - strand, within H281DRAFT_02146 at 288.217 kb on - strand, within H281DRAFT_02146 at 288.217 kb on - strand, within H281DRAFT_02146 at 288.217 kb on - strand, within H281DRAFT_02146 at 288.218 kb on + strand, within H281DRAFT_02146 at 288.219 kb on - strand, within H281DRAFT_02146 at 288.219 kb on - strand, within H281DRAFT_02146
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 286,380 + H281DRAFT_02144 0.53 +0.3 286,380 + H281DRAFT_02144 0.53 -3.2 286,380 + H281DRAFT_02144 0.53 -0.9 286,381 - H281DRAFT_02144 0.53 -0.0 286,381 - H281DRAFT_02144 0.53 -0.7 286,684 + H281DRAFT_02144 0.74 +0.3 286,909 + -1.3 286,909 + +0.5 286,909 + -1.1 286,909 + +1.9 286,961 + -0.3 286,961 + -0.0 286,961 + +0.6 286,962 - -0.8 287,049 + +0.5 287,050 - +0.6 287,050 - -0.2 287,107 + -0.2 287,141 + +0.6 287,163 + -0.9 287,264 - +0.1 287,304 + +0.7 287,320 - -0.1 287,393 - H281DRAFT_02145 0.56 -1.1 287,559 + +0.5 287,559 + -0.4 287,559 + -0.4 287,560 - -0.0 287,560 - +0.1 287,560 - -0.1 287,560 - +0.1 287,560 - -0.5 287,583 + +0.2 287,653 + +0.1 287,653 + +0.1 287,654 - -0.8 287,654 - +0.8 287,654 - +0.3 287,654 - -1.7 287,654 - -0.6 287,654 - +2.9 287,654 - +0.0 287,654 - -0.1 287,662 - +0.0 287,662 - -0.3 287,662 - -1.7 287,739 + -0.7 287,740 - -0.2 287,775 + +1.9 287,850 - +0.3 287,850 - +0.1 287,906 + +0.5 287,906 + -0.6 287,906 + -0.0 287,907 - -1.0 287,907 - -0.5 287,907 - -0.2 287,913 + H281DRAFT_02146 0.10 +0.4 287,914 - H281DRAFT_02146 0.10 -0.3 288,111 + H281DRAFT_02146 0.26 -0.5 288,112 - H281DRAFT_02146 0.26 -0.1 288,184 + H281DRAFT_02146 0.32 -0.1 288,185 - H281DRAFT_02146 0.32 +0.6 288,217 - H281DRAFT_02146 0.35 -0.8 288,217 - H281DRAFT_02146 0.35 +0.1 288,217 - H281DRAFT_02146 0.35 -0.4 288,217 - H281DRAFT_02146 0.35 -0.3 288,217 - H281DRAFT_02146 0.35 -0.7 288,218 + H281DRAFT_02146 0.35 -1.9 288,219 - H281DRAFT_02146 0.35 -0.7 288,219 - H281DRAFT_02146 0.35 -0.5
Or see this region's nucleotide sequence