Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_01174 and H281DRAFT_01175 are separated by 182 nucleotides H281DRAFT_01175 and H281DRAFT_01176 are separated by 163 nucleotides H281DRAFT_01176 and H281DRAFT_01177 are separated by 4 nucleotides
H281DRAFT_01174: H281DRAFT_01174 - Flavin-dependent oxidoreductase, luciferase family (includes alkanesulfonate monooxygenase SsuD and methylene tetrahydromethanopterin reductase), at 8,200 to 9,261
_01174
H281DRAFT_01175: H281DRAFT_01175 - hypothetical protein, at 9,444 to 9,623
_01175
H281DRAFT_01176: H281DRAFT_01176 - fatty-acyl-CoA synthase, at 9,787 to 11,442
_01176
H281DRAFT_01177: H281DRAFT_01177 - NAD(P)-dependent dehydrogenase, short-chain alcohol dehydrogenase family, at 11,447 to 12,685
_01177
Position (kb)
9
10
11
12 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 8.823 kb on - strand, within H281DRAFT_01174 at 8.866 kb on + strand, within H281DRAFT_01174 at 8.866 kb on + strand, within H281DRAFT_01174 at 8.977 kb on - strand, within H281DRAFT_01174 at 8.977 kb on - strand, within H281DRAFT_01174 at 9.113 kb on + strand, within H281DRAFT_01174 at 9.114 kb on - strand, within H281DRAFT_01174 at 9.402 kb on - strand at 9.436 kb on - strand at 9.436 kb on - strand at 9.850 kb on + strand at 10.005 kb on + strand, within H281DRAFT_01176 at 10.028 kb on - strand, within H281DRAFT_01176 at 10.028 kb on - strand, within H281DRAFT_01176 at 10.030 kb on + strand, within H281DRAFT_01176 at 10.100 kb on - strand, within H281DRAFT_01176 at 10.291 kb on + strand, within H281DRAFT_01176 at 10.293 kb on + strand, within H281DRAFT_01176 at 10.293 kb on + strand, within H281DRAFT_01176 at 10.293 kb on + strand, within H281DRAFT_01176 at 10.294 kb on - strand, within H281DRAFT_01176 at 10.294 kb on - strand, within H281DRAFT_01176 at 10.614 kb on + strand at 11.446 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 8,823 - H281DRAFT_01174 0.59 +0.2 8,866 + H281DRAFT_01174 0.63 +0.2 8,866 + H281DRAFT_01174 0.63 -0.1 8,977 - H281DRAFT_01174 0.73 -0.1 8,977 - H281DRAFT_01174 0.73 +0.7 9,113 + H281DRAFT_01174 0.86 +1.0 9,114 - H281DRAFT_01174 0.86 -0.4 9,402 - -0.3 9,436 - -0.2 9,436 - -0.2 9,850 + +1.3 10,005 + H281DRAFT_01176 0.13 -0.5 10,028 - H281DRAFT_01176 0.15 -0.3 10,028 - H281DRAFT_01176 0.15 -0.6 10,030 + H281DRAFT_01176 0.15 -3.1 10,100 - H281DRAFT_01176 0.19 -0.2 10,291 + H281DRAFT_01176 0.30 +1.7 10,293 + H281DRAFT_01176 0.31 -1.2 10,293 + H281DRAFT_01176 0.31 +0.5 10,293 + H281DRAFT_01176 0.31 -0.5 10,294 - H281DRAFT_01176 0.31 +0.8 10,294 - H281DRAFT_01176 0.31 +0.8 10,614 + +2.3 11,446 + -2.2
Or see this region's nucleotide sequence