Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00649 and H281DRAFT_00650 are separated by 64 nucleotides H281DRAFT_00650 and H281DRAFT_00651 are separated by 91 nucleotides H281DRAFT_00651 and H281DRAFT_00652 are separated by 103 nucleotides
H281DRAFT_00649: H281DRAFT_00649 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 727,302 to 728,303
_00649
H281DRAFT_00650: H281DRAFT_00650 - transcriptional regulator, DeoR family, at 728,368 to 729,204
_00650
H281DRAFT_00651: H281DRAFT_00651 - hypothetical protein, at 729,296 to 729,715
_00651
H281DRAFT_00652: H281DRAFT_00652 - Glycosyltransferase involved in cell wall bisynthesis, at 729,819 to 730,586
_00652
Position (kb) 729
730 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 728.334 kb on - strand at 728.334 kb on - strand at 728.521 kb on + strand, within H281DRAFT_00650 at 728.649 kb on + strand, within H281DRAFT_00650 at 729.057 kb on + strand, within H281DRAFT_00650 at 729.058 kb on - strand, within H281DRAFT_00650 at 729.138 kb on + strand at 729.139 kb on - strand at 729.208 kb on - strand at 729.244 kb on + strand at 729.244 kb on + strand at 729.245 kb on - strand at 729.694 kb on - strand at 729.697 kb on + strand at 729.697 kb on + strand at 729.698 kb on - strand at 729.715 kb on + strand at 729.715 kb on + strand at 729.871 kb on + strand at 729.871 kb on + strand at 729.883 kb on + strand at 729.883 kb on + strand at 729.937 kb on + strand, within H281DRAFT_00652 at 730.082 kb on + strand, within H281DRAFT_00652 at 730.149 kb on - strand, within H281DRAFT_00652 at 730.149 kb on - strand, within H281DRAFT_00652 at 730.149 kb on - strand, within H281DRAFT_00652 at 730.268 kb on - strand, within H281DRAFT_00652 at 730.268 kb on - strand, within H281DRAFT_00652 at 730.305 kb on + strand, within H281DRAFT_00652 at 730.567 kb on + strand at 730.600 kb on + strand at 730.603 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 728,334 - -0.3 728,334 - -0.4 728,521 + H281DRAFT_00650 0.18 +1.2 728,649 + H281DRAFT_00650 0.34 -0.0 729,057 + H281DRAFT_00650 0.82 -0.4 729,058 - H281DRAFT_00650 0.82 +0.6 729,138 + +0.9 729,139 - +0.8 729,208 - -1.1 729,244 + +0.2 729,244 + +0.3 729,245 - -0.6 729,694 - -1.3 729,697 + -0.4 729,697 + +0.3 729,698 - +0.1 729,715 + -1.7 729,715 + +1.5 729,871 + -0.4 729,871 + -1.2 729,883 + -0.3 729,883 + +0.1 729,937 + H281DRAFT_00652 0.15 -2.9 730,082 + H281DRAFT_00652 0.34 +1.0 730,149 - H281DRAFT_00652 0.43 -0.6 730,149 - H281DRAFT_00652 0.43 -1.3 730,149 - H281DRAFT_00652 0.43 -1.8 730,268 - H281DRAFT_00652 0.58 +0.2 730,268 - H281DRAFT_00652 0.58 +0.4 730,305 + H281DRAFT_00652 0.63 +0.7 730,567 + +0.3 730,600 + -1.3 730,603 + -0.8
Or see this region's nucleotide sequence