Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00177 and H281DRAFT_00178 are separated by 193 nucleotides H281DRAFT_00178 and H281DRAFT_00179 are separated by 92 nucleotides
H281DRAFT_00177: H281DRAFT_00177 - transcriptional regulator, LysR family, at 190,952 to 191,863
_00177
H281DRAFT_00178: H281DRAFT_00178 - agmatinase, at 192,057 to 193,046
_00178
H281DRAFT_00179: H281DRAFT_00179 - nucleobase:cation symporter-1, NCS1 family, at 193,139 to 194,455
_00179
Position (kb)
192
193
194 Strain fitness (log2 ratio)
-1
0
1 at 191.069 kb on + strand, within H281DRAFT_00177 at 191.069 kb on + strand, within H281DRAFT_00177 at 191.070 kb on - strand, within H281DRAFT_00177 at 191.070 kb on - strand, within H281DRAFT_00177 at 191.244 kb on + strand, within H281DRAFT_00177 at 191.244 kb on + strand, within H281DRAFT_00177 at 191.245 kb on - strand, within H281DRAFT_00177 at 191.364 kb on + strand, within H281DRAFT_00177 at 191.364 kb on + strand, within H281DRAFT_00177 at 191.364 kb on + strand, within H281DRAFT_00177 at 191.429 kb on + strand, within H281DRAFT_00177 at 191.515 kb on + strand, within H281DRAFT_00177 at 191.515 kb on + strand, within H281DRAFT_00177 at 191.515 kb on + strand, within H281DRAFT_00177 at 191.516 kb on - strand, within H281DRAFT_00177 at 191.917 kb on - strand at 192.062 kb on + strand at 192.295 kb on - strand, within H281DRAFT_00178 at 192.295 kb on - strand, within H281DRAFT_00178 at 192.370 kb on - strand, within H281DRAFT_00178 at 192.418 kb on + strand, within H281DRAFT_00178 at 192.419 kb on - strand, within H281DRAFT_00178 at 192.419 kb on - strand, within H281DRAFT_00178 at 192.419 kb on - strand, within H281DRAFT_00178 at 192.660 kb on + strand, within H281DRAFT_00178 at 192.789 kb on + strand, within H281DRAFT_00178 at 192.789 kb on + strand, within H281DRAFT_00178 at 192.790 kb on - strand, within H281DRAFT_00178 at 192.791 kb on + strand, within H281DRAFT_00178 at 193.121 kb on + strand at 193.157 kb on - strand at 193.179 kb on + strand at 193.179 kb on + strand at 193.179 kb on - strand at 193.252 kb on - strand at 193.350 kb on - strand, within H281DRAFT_00179 at 193.456 kb on + strand, within H281DRAFT_00179 at 193.456 kb on + strand, within H281DRAFT_00179 at 193.456 kb on + strand, within H281DRAFT_00179 at 193.457 kb on - strand, within H281DRAFT_00179 at 193.674 kb on + strand, within H281DRAFT_00179 at 193.675 kb on - strand, within H281DRAFT_00179 at 193.675 kb on - strand, within H281DRAFT_00179 at 193.770 kb on + strand, within H281DRAFT_00179
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 191,069 + H281DRAFT_00177 0.13 +1.4 191,069 + H281DRAFT_00177 0.13 +0.5 191,070 - H281DRAFT_00177 0.13 -0.4 191,070 - H281DRAFT_00177 0.13 -0.2 191,244 + H281DRAFT_00177 0.32 +0.6 191,244 + H281DRAFT_00177 0.32 +1.1 191,245 - H281DRAFT_00177 0.32 +0.3 191,364 + H281DRAFT_00177 0.45 +0.0 191,364 + H281DRAFT_00177 0.45 -1.2 191,364 + H281DRAFT_00177 0.45 -0.9 191,429 + H281DRAFT_00177 0.52 -0.6 191,515 + H281DRAFT_00177 0.62 +0.3 191,515 + H281DRAFT_00177 0.62 +0.4 191,515 + H281DRAFT_00177 0.62 -0.5 191,516 - H281DRAFT_00177 0.62 +1.6 191,917 - -1.5 192,062 + +0.1 192,295 - H281DRAFT_00178 0.24 -0.5 192,295 - H281DRAFT_00178 0.24 -0.7 192,370 - H281DRAFT_00178 0.32 +0.4 192,418 + H281DRAFT_00178 0.36 -1.1 192,419 - H281DRAFT_00178 0.37 -0.7 192,419 - H281DRAFT_00178 0.37 -0.9 192,419 - H281DRAFT_00178 0.37 -0.7 192,660 + H281DRAFT_00178 0.61 +0.4 192,789 + H281DRAFT_00178 0.74 +0.8 192,789 + H281DRAFT_00178 0.74 -0.0 192,790 - H281DRAFT_00178 0.74 +1.0 192,791 + H281DRAFT_00178 0.74 -0.2 193,121 + -0.6 193,157 - +0.4 193,179 + +0.3 193,179 + -0.5 193,179 - -0.4 193,252 - -1.0 193,350 - H281DRAFT_00179 0.16 +0.1 193,456 + H281DRAFT_00179 0.24 -0.2 193,456 + H281DRAFT_00179 0.24 +0.7 193,456 + H281DRAFT_00179 0.24 +0.9 193,457 - H281DRAFT_00179 0.24 -0.4 193,674 + H281DRAFT_00179 0.41 -0.3 193,675 - H281DRAFT_00179 0.41 -1.1 193,675 - H281DRAFT_00179 0.41 -0.2 193,770 + H281DRAFT_00179 0.48 -0.2
Or see this region's nucleotide sequence