Experiment: LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_00034 and H281DRAFT_00035 are separated by 547 nucleotides H281DRAFT_00035 and H281DRAFT_00036 are separated by 549 nucleotides
H281DRAFT_00034: H281DRAFT_00034 - hypothetical protein, at 36,317 to 36,478
_00034
H281DRAFT_00035: H281DRAFT_00035 - Phenylpyruvate tautomerase PptA, 4-oxalocrotonate tautomerase family, at 37,026 to 37,439
_00035
H281DRAFT_00036: H281DRAFT_00036 - DNA-binding winged helix-turn-helix (wHTH) domain-containing protein, at 37,989 to 40,754
_00036
Position (kb) 37
38 Strain fitness (log2 ratio) -2
-1
0
1 at 36.028 kb on + strand at 36.148 kb on + strand at 36.151 kb on - strand at 36.259 kb on - strand at 36.292 kb on - strand at 36.333 kb on - strand at 36.340 kb on + strand, within H281DRAFT_00034 at 36.478 kb on + strand at 36.479 kb on - strand at 36.572 kb on + strand at 36.580 kb on + strand at 36.643 kb on + strand at 36.771 kb on + strand at 36.771 kb on + strand at 36.772 kb on - strand at 36.838 kb on - strand at 36.901 kb on - strand at 36.901 kb on - strand at 36.904 kb on + strand at 36.904 kb on + strand at 36.905 kb on - strand at 36.905 kb on - strand at 36.972 kb on + strand at 36.972 kb on + strand at 36.973 kb on - strand at 37.027 kb on + strand at 37.028 kb on - strand at 37.069 kb on + strand, within H281DRAFT_00035 at 37.069 kb on + strand, within H281DRAFT_00035 at 37.069 kb on + strand, within H281DRAFT_00035 at 37.070 kb on - strand, within H281DRAFT_00035 at 37.070 kb on - strand, within H281DRAFT_00035 at 37.070 kb on - strand, within H281DRAFT_00035 at 37.070 kb on - strand, within H281DRAFT_00035 at 37.093 kb on - strand, within H281DRAFT_00035 at 37.111 kb on + strand, within H281DRAFT_00035 at 37.244 kb on + strand, within H281DRAFT_00035 at 37.267 kb on + strand, within H281DRAFT_00035 at 37.268 kb on - strand, within H281DRAFT_00035 at 37.489 kb on + strand at 37.544 kb on + strand at 37.545 kb on - strand at 37.566 kb on - strand at 37.566 kb on - strand at 37.805 kb on - strand at 37.807 kb on + strand at 37.807 kb on + strand at 37.874 kb on + strand at 37.939 kb on + strand at 38.089 kb on - strand at 38.121 kb on + strand at 38.121 kb on + strand at 38.121 kb on + strand at 38.122 kb on - strand at 38.122 kb on - strand at 38.127 kb on + strand at 38.145 kb on + strand at 38.148 kb on + strand at 38.296 kb on + strand, within H281DRAFT_00036 at 38.297 kb on - strand, within H281DRAFT_00036 at 38.297 kb on - strand, within H281DRAFT_00036
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x remove 36,028 + +0.1 36,148 + +0.4 36,151 - -0.2 36,259 - -0.1 36,292 - -0.8 36,333 - -1.4 36,340 + H281DRAFT_00034 0.14 +0.1 36,478 + -0.9 36,479 - -2.1 36,572 + +0.9 36,580 + -0.3 36,643 + -0.2 36,771 + -0.0 36,771 + -0.8 36,772 - -0.2 36,838 - -0.8 36,901 - -0.7 36,901 - -0.3 36,904 + +0.3 36,904 + -0.2 36,905 - -1.5 36,905 - +0.5 36,972 + +0.3 36,972 + +0.0 36,973 - -0.7 37,027 + +0.2 37,028 - -0.3 37,069 + H281DRAFT_00035 0.10 +0.1 37,069 + H281DRAFT_00035 0.10 +0.1 37,069 + H281DRAFT_00035 0.10 -0.6 37,070 - H281DRAFT_00035 0.11 +0.8 37,070 - H281DRAFT_00035 0.11 -0.1 37,070 - H281DRAFT_00035 0.11 -0.3 37,070 - H281DRAFT_00035 0.11 -0.6 37,093 - H281DRAFT_00035 0.16 -0.2 37,111 + H281DRAFT_00035 0.21 -1.6 37,244 + H281DRAFT_00035 0.53 +0.7 37,267 + H281DRAFT_00035 0.58 +0.0 37,268 - H281DRAFT_00035 0.58 -1.0 37,489 + -0.4 37,544 + +0.2 37,545 - +0.2 37,566 - -0.4 37,566 - +0.6 37,805 - +0.1 37,807 + -1.1 37,807 + +1.5 37,874 + +0.4 37,939 + +0.9 38,089 - +0.3 38,121 + +1.2 38,121 + +0.5 38,121 + -0.4 38,122 - +0.4 38,122 - -0.3 38,127 + +0.1 38,145 + +0.2 38,148 + -0.4 38,296 + H281DRAFT_00036 0.11 +0.1 38,297 - H281DRAFT_00036 0.11 -0.7 38,297 - H281DRAFT_00036 0.11 +0.2
Or see this region's nucleotide sequence