Experiment: Polymyxin B sulfate 4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_18830 and AO353_18835 are separated by 0 nucleotides AO353_18835 and AO353_18840 are separated by 202 nucleotides AO353_18840 and AO353_18845 overlap by 4 nucleotides
AO353_18830: AO353_18830 - chemotaxis protein CheW, at 4,140,287 to 4,140,772
_18830
AO353_18835: AO353_18835 - chemotaxis protein, at 4,140,773 to 4,141,165
_18835
AO353_18840: AO353_18840 - flagellar biosynthesis protein FlhB, at 4,141,368 to 4,141,697
_18840
AO353_18845: AO353_18845 - flagellar hook-length control protein FliK, at 4,141,694 to 4,143,265
_18845
Position (kb)
4141
4142 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4140.368 kb on + strand, within AO353_18830 at 4140.368 kb on + strand, within AO353_18830 at 4140.368 kb on + strand, within AO353_18830 at 4140.368 kb on + strand, within AO353_18830 at 4140.369 kb on - strand, within AO353_18830 at 4140.369 kb on - strand, within AO353_18830 at 4140.408 kb on - strand, within AO353_18830 at 4140.443 kb on + strand, within AO353_18830 at 4140.443 kb on + strand, within AO353_18830 at 4140.443 kb on + strand, within AO353_18830 at 4140.443 kb on + strand, within AO353_18830 at 4140.443 kb on + strand, within AO353_18830 at 4140.443 kb on + strand, within AO353_18830 at 4140.443 kb on + strand, within AO353_18830 at 4140.444 kb on - strand, within AO353_18830 at 4140.444 kb on - strand, within AO353_18830 at 4140.444 kb on - strand, within AO353_18830 at 4140.444 kb on - strand, within AO353_18830 at 4140.444 kb on - strand, within AO353_18830 at 4140.611 kb on + strand, within AO353_18830 at 4140.611 kb on + strand, within AO353_18830 at 4140.611 kb on + strand, within AO353_18830 at 4140.643 kb on + strand, within AO353_18830 at 4140.766 kb on + strand at 4140.766 kb on + strand at 4140.766 kb on + strand at 4140.766 kb on + strand at 4140.766 kb on + strand at 4140.767 kb on - strand at 4140.767 kb on - strand at 4140.767 kb on - strand at 4140.795 kb on + strand at 4140.795 kb on + strand at 4140.795 kb on + strand at 4140.795 kb on + strand at 4140.796 kb on - strand at 4140.796 kb on - strand at 4140.796 kb on - strand at 4140.798 kb on + strand at 4140.799 kb on - strand at 4140.799 kb on - strand at 4140.799 kb on - strand at 4140.842 kb on + strand, within AO353_18835 at 4140.843 kb on - strand, within AO353_18835 at 4140.843 kb on - strand, within AO353_18835 at 4141.256 kb on + strand at 4141.256 kb on + strand at 4141.256 kb on + strand at 4141.256 kb on + strand at 4141.256 kb on + strand at 4141.257 kb on - strand at 4141.257 kb on - strand at 4141.257 kb on - strand at 4141.257 kb on - strand at 4141.257 kb on - strand at 4141.257 kb on - strand at 4141.257 kb on - strand at 4141.257 kb on - strand at 4141.257 kb on - strand at 4141.257 kb on - strand at 4141.270 kb on + strand at 4141.270 kb on + strand at 4141.373 kb on - strand at 4141.373 kb on - strand at 4141.373 kb on - strand at 4141.373 kb on - strand at 4141.489 kb on + strand, within AO353_18840 at 4141.490 kb on - strand, within AO353_18840 at 4141.558 kb on + strand, within AO353_18840 at 4141.558 kb on + strand, within AO353_18840 at 4141.558 kb on + strand, within AO353_18840 at 4141.559 kb on - strand, within AO353_18840 at 4141.573 kb on + strand, within AO353_18840 at 4141.574 kb on - strand, within AO353_18840 at 4141.654 kb on + strand, within AO353_18840 at 4141.654 kb on + strand, within AO353_18840 at 4141.654 kb on + strand, within AO353_18840 at 4141.655 kb on - strand, within AO353_18840 at 4141.847 kb on - strand at 4141.847 kb on - strand at 4141.848 kb on + strand at 4141.848 kb on + strand at 4141.848 kb on + strand at 4141.848 kb on + strand at 4141.849 kb on - strand at 4141.849 kb on - strand at 4141.849 kb on - strand at 4141.849 kb on - strand at 4141.849 kb on - strand at 4141.849 kb on - strand at 4142.356 kb on + strand, within AO353_18845 at 4142.356 kb on + strand, within AO353_18845 at 4142.357 kb on - strand, within AO353_18845 at 4142.357 kb on - strand, within AO353_18845
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 4 mg/ml remove 4,140,368 + AO353_18830 0.17 +0.0 4,140,368 + AO353_18830 0.17 +0.5 4,140,368 + AO353_18830 0.17 +0.8 4,140,368 + AO353_18830 0.17 -0.1 4,140,369 - AO353_18830 0.17 -1.1 4,140,369 - AO353_18830 0.17 +0.2 4,140,408 - AO353_18830 0.25 +0.1 4,140,443 + AO353_18830 0.32 -0.0 4,140,443 + AO353_18830 0.32 -1.5 4,140,443 + AO353_18830 0.32 +0.5 4,140,443 + AO353_18830 0.32 +0.0 4,140,443 + AO353_18830 0.32 +1.3 4,140,443 + AO353_18830 0.32 +0.9 4,140,443 + AO353_18830 0.32 +0.2 4,140,444 - AO353_18830 0.32 +0.2 4,140,444 - AO353_18830 0.32 +0.5 4,140,444 - AO353_18830 0.32 +0.3 4,140,444 - AO353_18830 0.32 -1.7 4,140,444 - AO353_18830 0.32 -0.1 4,140,611 + AO353_18830 0.67 -0.8 4,140,611 + AO353_18830 0.67 -1.1 4,140,611 + AO353_18830 0.67 -0.8 4,140,643 + AO353_18830 0.73 +0.5 4,140,766 + -1.3 4,140,766 + +0.5 4,140,766 + +1.7 4,140,766 + +0.7 4,140,766 + -0.4 4,140,767 - +0.7 4,140,767 - +0.4 4,140,767 - +0.2 4,140,795 + -0.6 4,140,795 + +0.3 4,140,795 + +0.3 4,140,795 + +0.6 4,140,796 - +1.1 4,140,796 - +0.0 4,140,796 - -0.0 4,140,798 + -1.2 4,140,799 - +0.6 4,140,799 - -0.5 4,140,799 - -0.4 4,140,842 + AO353_18835 0.18 -1.3 4,140,843 - AO353_18835 0.18 -0.7 4,140,843 - AO353_18835 0.18 +1.0 4,141,256 + -1.8 4,141,256 + -1.3 4,141,256 + +0.3 4,141,256 + +1.5 4,141,256 + +1.0 4,141,257 - -1.1 4,141,257 - -1.2 4,141,257 - +1.0 4,141,257 - -1.3 4,141,257 - -0.8 4,141,257 - -0.4 4,141,257 - -0.3 4,141,257 - +0.1 4,141,257 - -0.1 4,141,257 - +0.5 4,141,270 + +1.1 4,141,270 + +0.3 4,141,373 - -1.0 4,141,373 - -1.5 4,141,373 - -1.1 4,141,373 - +0.5 4,141,489 + AO353_18840 0.37 +1.3 4,141,490 - AO353_18840 0.37 +0.9 4,141,558 + AO353_18840 0.58 -0.5 4,141,558 + AO353_18840 0.58 +0.3 4,141,558 + AO353_18840 0.58 +0.5 4,141,559 - AO353_18840 0.58 +0.2 4,141,573 + AO353_18840 0.62 +0.3 4,141,574 - AO353_18840 0.62 +0.8 4,141,654 + AO353_18840 0.87 -0.3 4,141,654 + AO353_18840 0.87 +1.0 4,141,654 + AO353_18840 0.87 +0.6 4,141,655 - AO353_18840 0.87 -0.3 4,141,847 - -0.5 4,141,847 - +0.2 4,141,848 + +1.8 4,141,848 + -1.1 4,141,848 + +0.8 4,141,848 + +1.0 4,141,849 - -0.4 4,141,849 - -0.2 4,141,849 - -1.1 4,141,849 - -0.6 4,141,849 - -0.2 4,141,849 - +0.3 4,142,356 + AO353_18845 0.42 +0.0 4,142,356 + AO353_18845 0.42 -0.6 4,142,357 - AO353_18845 0.42 +0.7 4,142,357 - AO353_18845 0.42 +0.7
Or see this region's nucleotide sequence