Experiment: Polymyxin B sulfate 4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_17925 and AO353_17930 are separated by 151 nucleotides AO353_17930 and AO353_17935 are separated by 49 nucleotides AO353_17935 and AO353_17940 are separated by 118 nucleotides AO353_17940 and AO353_17945 overlap by 4 nucleotides AO353_17945 and AO353_17950 are separated by 115 nucleotides
AO353_17925: AO353_17925 - transcriptional regulator, at 3,947,419 to 3,948,102
_17925
AO353_17930: AO353_17930 - restriction endonuclease, at 3,948,254 to 3,948,628
_17930
AO353_17935: AO353_17935 - hypothetical protein, at 3,948,678 to 3,949,364
_17935
AO353_17940: AO353_17940 - hypothetical protein, at 3,949,483 to 3,949,773
_17940
AO353_17945: AO353_17945 - cyclic nucleotide-binding protein, at 3,949,770 to 3,950,243
_17945
AO353_17950: AO353_17950 - peptidase S9, at 3,950,359 to 3,952,413
_17950
Position (kb)
3948
3949
3950 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3947.746 kb on - strand, within AO353_17925 at 3947.746 kb on - strand, within AO353_17925 at 3947.807 kb on + strand, within AO353_17925 at 3947.807 kb on + strand, within AO353_17925 at 3947.807 kb on + strand, within AO353_17925 at 3947.808 kb on - strand, within AO353_17925 at 3947.808 kb on - strand, within AO353_17925 at 3947.808 kb on - strand, within AO353_17925 at 3947.966 kb on + strand, within AO353_17925 at 3947.966 kb on + strand, within AO353_17925 at 3947.966 kb on + strand, within AO353_17925 at 3947.966 kb on + strand, within AO353_17925 at 3947.966 kb on + strand, within AO353_17925 at 3947.967 kb on - strand, within AO353_17925 at 3947.967 kb on - strand, within AO353_17925 at 3947.967 kb on - strand, within AO353_17925 at 3948.082 kb on + strand at 3948.082 kb on + strand at 3948.083 kb on - strand at 3948.083 kb on - strand at 3948.110 kb on - strand at 3948.113 kb on + strand at 3948.114 kb on - strand at 3948.114 kb on - strand at 3948.114 kb on - strand at 3948.114 kb on - strand at 3948.114 kb on - strand at 3948.191 kb on + strand at 3948.191 kb on + strand at 3948.192 kb on - strand at 3948.192 kb on - strand at 3948.207 kb on + strand at 3948.299 kb on - strand, within AO353_17930 at 3948.348 kb on + strand, within AO353_17930 at 3948.348 kb on + strand, within AO353_17930 at 3948.348 kb on + strand, within AO353_17930 at 3948.349 kb on - strand, within AO353_17930 at 3948.349 kb on - strand, within AO353_17930 at 3948.349 kb on - strand, within AO353_17930 at 3948.349 kb on - strand, within AO353_17930 at 3948.390 kb on + strand, within AO353_17930 at 3948.390 kb on + strand, within AO353_17930 at 3948.390 kb on + strand, within AO353_17930 at 3948.430 kb on + strand, within AO353_17930 at 3948.522 kb on + strand, within AO353_17930 at 3948.522 kb on + strand, within AO353_17930 at 3948.523 kb on - strand, within AO353_17930 at 3948.643 kb on - strand at 3948.652 kb on - strand at 3948.664 kb on - strand at 3948.674 kb on + strand at 3948.674 kb on + strand at 3948.674 kb on + strand at 3948.675 kb on - strand at 3948.675 kb on - strand at 3948.675 kb on - strand at 3948.675 kb on - strand at 3948.816 kb on + strand, within AO353_17935 at 3948.877 kb on + strand, within AO353_17935 at 3948.877 kb on + strand, within AO353_17935 at 3948.877 kb on + strand, within AO353_17935 at 3948.877 kb on + strand at 3949.295 kb on - strand, within AO353_17935 at 3949.376 kb on + strand at 3949.376 kb on + strand at 3949.377 kb on - strand at 3949.451 kb on + strand at 3949.499 kb on + strand at 3949.499 kb on + strand at 3949.500 kb on - strand at 3949.500 kb on - strand at 3949.529 kb on + strand, within AO353_17940 at 3949.530 kb on - strand, within AO353_17940 at 3949.530 kb on - strand, within AO353_17940 at 3949.530 kb on - strand, within AO353_17940 at 3949.611 kb on + strand, within AO353_17940 at 3949.611 kb on + strand, within AO353_17940 at 3949.618 kb on + strand, within AO353_17940 at 3949.630 kb on - strand, within AO353_17940 at 3949.644 kb on - strand, within AO353_17940 at 3949.644 kb on - strand, within AO353_17940 at 3949.644 kb on - strand, within AO353_17940 at 3949.771 kb on + strand at 3949.835 kb on + strand, within AO353_17945 at 3949.836 kb on - strand, within AO353_17945 at 3950.031 kb on - strand, within AO353_17945 at 3950.140 kb on + strand, within AO353_17945 at 3950.141 kb on - strand, within AO353_17945 at 3950.291 kb on + strand at 3950.291 kb on + strand at 3950.292 kb on - strand at 3950.292 kb on - strand at 3950.292 kb on - strand at 3950.332 kb on + strand at 3950.332 kb on + strand at 3950.332 kb on + strand at 3950.332 kb on + strand at 3950.332 kb on + strand at 3950.332 kb on + strand at 3950.332 kb on + strand at 3950.332 kb on + strand at 3950.333 kb on - strand at 3950.333 kb on - strand at 3950.333 kb on - strand at 3950.333 kb on - strand at 3950.333 kb on - strand at 3950.341 kb on + strand at 3950.341 kb on + strand at 3950.342 kb on - strand at 3950.342 kb on - strand at 3950.342 kb on - strand at 3950.342 kb on - strand at 3950.342 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 4 mg/ml remove 3,947,746 - AO353_17925 0.48 -0.3 3,947,746 - AO353_17925 0.48 +0.6 3,947,807 + AO353_17925 0.57 -0.4 3,947,807 + AO353_17925 0.57 -2.6 3,947,807 + AO353_17925 0.57 -0.1 3,947,808 - AO353_17925 0.57 +0.8 3,947,808 - AO353_17925 0.57 -0.7 3,947,808 - AO353_17925 0.57 -0.6 3,947,966 + AO353_17925 0.80 -0.3 3,947,966 + AO353_17925 0.80 -0.4 3,947,966 + AO353_17925 0.80 -0.0 3,947,966 + AO353_17925 0.80 +0.8 3,947,966 + AO353_17925 0.80 +1.0 3,947,967 - AO353_17925 0.80 -1.9 3,947,967 - AO353_17925 0.80 -0.2 3,947,967 - AO353_17925 0.80 -0.8 3,948,082 + +0.3 3,948,082 + -1.2 3,948,083 - +0.3 3,948,083 - +1.2 3,948,110 - +0.8 3,948,113 + -0.0 3,948,114 - +0.6 3,948,114 - +0.6 3,948,114 - +0.1 3,948,114 - -1.4 3,948,114 - -0.0 3,948,191 + -0.4 3,948,191 + -0.0 3,948,192 - +1.3 3,948,192 - -0.7 3,948,207 + +0.5 3,948,299 - AO353_17930 0.12 +1.6 3,948,348 + AO353_17930 0.25 +0.8 3,948,348 + AO353_17930 0.25 +0.7 3,948,348 + AO353_17930 0.25 -0.5 3,948,349 - AO353_17930 0.25 +1.2 3,948,349 - AO353_17930 0.25 -0.1 3,948,349 - AO353_17930 0.25 -1.3 3,948,349 - AO353_17930 0.25 +0.2 3,948,390 + AO353_17930 0.36 +1.3 3,948,390 + AO353_17930 0.36 +0.1 3,948,390 + AO353_17930 0.36 +0.8 3,948,430 + AO353_17930 0.47 +0.1 3,948,522 + AO353_17930 0.71 -0.7 3,948,522 + AO353_17930 0.71 +0.5 3,948,523 - AO353_17930 0.72 +1.1 3,948,643 - -1.6 3,948,652 - -0.3 3,948,664 - -0.3 3,948,674 + -1.1 3,948,674 + +0.9 3,948,674 + -0.0 3,948,675 - -1.5 3,948,675 - -0.4 3,948,675 - +1.6 3,948,675 - -0.7 3,948,816 + AO353_17935 0.20 +0.8 3,948,877 + AO353_17935 0.29 -1.4 3,948,877 + AO353_17935 0.29 +0.1 3,948,877 + AO353_17935 0.29 -1.1 3,948,877 + +1.0 3,949,295 - AO353_17935 0.90 -0.8 3,949,376 + -0.9 3,949,376 + +1.0 3,949,377 - +0.7 3,949,451 + +0.3 3,949,499 + -0.6 3,949,499 + -0.5 3,949,500 - +1.4 3,949,500 - +0.2 3,949,529 + AO353_17940 0.16 +0.2 3,949,530 - AO353_17940 0.16 +0.6 3,949,530 - AO353_17940 0.16 -1.0 3,949,530 - AO353_17940 0.16 -0.3 3,949,611 + AO353_17940 0.44 -0.3 3,949,611 + AO353_17940 0.44 -0.4 3,949,618 + AO353_17940 0.46 -1.1 3,949,630 - AO353_17940 0.51 +0.6 3,949,644 - AO353_17940 0.55 +0.8 3,949,644 - AO353_17940 0.55 +0.4 3,949,644 - AO353_17940 0.55 +0.6 3,949,771 + +0.8 3,949,835 + AO353_17945 0.14 -1.5 3,949,836 - AO353_17945 0.14 +0.1 3,950,031 - AO353_17945 0.55 -0.4 3,950,140 + AO353_17945 0.78 -0.6 3,950,141 - AO353_17945 0.78 -1.8 3,950,291 + -0.3 3,950,291 + -0.6 3,950,292 - +0.3 3,950,292 - -0.8 3,950,292 - -0.9 3,950,332 + -1.3 3,950,332 + -0.1 3,950,332 + -1.2 3,950,332 + +1.0 3,950,332 + +0.2 3,950,332 + +0.2 3,950,332 + -1.8 3,950,332 + -0.3 3,950,333 - +0.2 3,950,333 - -0.2 3,950,333 - +1.1 3,950,333 - -0.3 3,950,333 - -1.6 3,950,341 + -0.7 3,950,341 + +0.0 3,950,342 - +2.6 3,950,342 - -0.8 3,950,342 - -2.3 3,950,342 - -0.4 3,950,342 - +2.1
Or see this region's nucleotide sequence