Experiment: Polymyxin B sulfate 4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_16470 and AO353_16475 overlap by 4 nucleotides AO353_16475 and AO353_16480 are separated by 17 nucleotides AO353_16480 and AO353_16485 overlap by 1 nucleotides AO353_16485 and AO353_16490 overlap by 4 nucleotides
AO353_16470: AO353_16470 - uridylate kinase, at 3,657,780 to 3,658,523
_16470
AO353_16475: AO353_16475 - ribosome-recycling factor, at 3,658,520 to 3,659,077
_16475
AO353_16480: AO353_16480 - farnesyl-diphosphate synthase, at 3,659,095 to 3,659,850
_16480
AO353_16485: AO353_16485 - phosphatidate cytidylyltransferase, at 3,659,850 to 3,660,656
_16485
AO353_16490: AO353_16490 - 1-deoxy-D-xylulose 5-phosphate reductoisomerase, at 3,660,653 to 3,661,843
_16490
Position (kb)
3659
3660 Strain fitness (log2 ratio)
-1
0
1
2 at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.073 kb on + strand at 3659.075 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 4 mg/ml remove 3,659,073 + +0.3 3,659,073 + -1.5 3,659,073 + +0.5 3,659,073 + +0.3 3,659,073 + -0.6 3,659,073 + +1.9 3,659,073 + +0.1 3,659,073 + -0.2 3,659,073 + +0.0 3,659,073 + +1.0 3,659,073 + +1.2 3,659,073 + +0.4 3,659,073 + -0.2 3,659,075 + +0.3
Or see this region's nucleotide sequence