Experiment: Polymyxin B sulfate 4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_02265 and AO353_02270 are separated by 238 nucleotides AO353_02270 and AO353_02275 are separated by 132 nucleotides AO353_02275 and AO353_02280 are separated by 75 nucleotides AO353_02280 and AO353_02285 are separated by 77 nucleotides
AO353_02265: AO353_02265 - protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific, at 484,246 to 485,154
_02265
AO353_02270: AO353_02270 - isochorismatase, at 485,393 to 485,983
_02270
AO353_02275: AO353_02275 - hypothetical protein, at 486,116 to 486,436
_02275
AO353_02280: AO353_02280 - DNA mismatch repair protein MutS, at 486,512 to 487,069
_02280
AO353_02285: AO353_02285 - GTP cyclohydrolase, at 487,147 to 487,692
_02285
Position (kb) 486
487 Strain fitness (log2 ratio) -2
-1
0
1 at 485.189 kb on + strand at 485.189 kb on + strand at 485.189 kb on + strand at 485.189 kb on + strand at 485.190 kb on - strand at 485.190 kb on - strand at 485.190 kb on - strand at 485.198 kb on - strand at 485.238 kb on + strand at 485.238 kb on + strand at 485.238 kb on + strand at 485.238 kb on + strand at 485.238 kb on + strand at 485.238 kb on + strand at 485.239 kb on - strand at 485.239 kb on - strand at 485.239 kb on - strand at 485.318 kb on + strand at 485.319 kb on - strand at 485.492 kb on + strand, within AO353_02270 at 485.492 kb on + strand, within AO353_02270 at 485.492 kb on + strand, within AO353_02270 at 485.542 kb on + strand, within AO353_02270 at 485.595 kb on + strand, within AO353_02270 at 485.595 kb on + strand, within AO353_02270 at 485.595 kb on + strand at 485.596 kb on - strand, within AO353_02270 at 485.596 kb on - strand, within AO353_02270 at 485.596 kb on - strand, within AO353_02270 at 485.596 kb on - strand, within AO353_02270 at 485.596 kb on - strand, within AO353_02270 at 485.596 kb on - strand, within AO353_02270 at 485.596 kb on - strand, within AO353_02270 at 485.596 kb on - strand, within AO353_02270 at 485.596 kb on - strand, within AO353_02270 at 485.803 kb on + strand, within AO353_02270 at 485.804 kb on - strand, within AO353_02270 at 485.804 kb on - strand, within AO353_02270 at 486.052 kb on + strand at 486.341 kb on + strand, within AO353_02275 at 486.341 kb on + strand, within AO353_02275 at 486.341 kb on + strand, within AO353_02275 at 486.341 kb on + strand, within AO353_02275 at 486.341 kb on + strand, within AO353_02275 at 486.341 kb on + strand, within AO353_02275 at 486.342 kb on - strand, within AO353_02275 at 486.342 kb on - strand, within AO353_02275 at 486.342 kb on - strand, within AO353_02275 at 486.342 kb on - strand, within AO353_02275 at 486.342 kb on - strand, within AO353_02275 at 486.342 kb on - strand, within AO353_02275 at 486.906 kb on + strand, within AO353_02280 at 486.906 kb on + strand, within AO353_02280 at 486.906 kb on + strand, within AO353_02280 at 486.906 kb on + strand, within AO353_02280 at 487.025 kb on + strand at 487.025 kb on + strand at 487.025 kb on + strand at 487.026 kb on - strand at 487.026 kb on - strand at 487.026 kb on - strand at 487.026 kb on - strand at 487.026 kb on - strand at 487.067 kb on + strand at 487.067 kb on + strand at 487.068 kb on - strand at 487.068 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 4 mg/ml remove 485,189 + -0.4 485,189 + -1.6 485,189 + +1.1 485,189 + +0.7 485,190 - -0.0 485,190 - -0.9 485,190 - -1.2 485,198 - -0.6 485,238 + -0.2 485,238 + -0.4 485,238 + -0.8 485,238 + +0.4 485,238 + -0.1 485,238 + -0.1 485,239 - -0.4 485,239 - +0.3 485,239 - +0.1 485,318 + -0.6 485,319 - +0.3 485,492 + AO353_02270 0.17 +1.0 485,492 + AO353_02270 0.17 +0.2 485,492 + AO353_02270 0.17 +0.8 485,542 + AO353_02270 0.25 +0.2 485,595 + AO353_02270 0.34 +0.8 485,595 + AO353_02270 0.34 -0.2 485,595 + +0.6 485,596 - AO353_02270 0.34 +0.7 485,596 - AO353_02270 0.34 +0.6 485,596 - AO353_02270 0.34 +0.2 485,596 - AO353_02270 0.34 -0.2 485,596 - AO353_02270 0.34 -1.8 485,596 - AO353_02270 0.34 -1.0 485,596 - AO353_02270 0.34 -1.3 485,596 - AO353_02270 0.34 -1.2 485,596 - AO353_02270 0.34 +0.4 485,803 + AO353_02270 0.69 -0.4 485,804 - AO353_02270 0.70 +0.1 485,804 - AO353_02270 0.70 -0.9 486,052 + -1.4 486,341 + AO353_02275 0.70 -0.3 486,341 + AO353_02275 0.70 -1.0 486,341 + AO353_02275 0.70 -1.3 486,341 + AO353_02275 0.70 -0.9 486,341 + AO353_02275 0.70 -1.2 486,341 + AO353_02275 0.70 +0.1 486,342 - AO353_02275 0.70 -0.4 486,342 - AO353_02275 0.70 -0.1 486,342 - AO353_02275 0.70 -1.2 486,342 - AO353_02275 0.70 -1.0 486,342 - AO353_02275 0.70 +0.1 486,342 - AO353_02275 0.70 -0.0 486,906 + AO353_02280 0.71 +1.2 486,906 + AO353_02280 0.71 -0.9 486,906 + AO353_02280 0.71 +1.2 486,906 + AO353_02280 0.71 +1.4 487,025 + -0.4 487,025 + +0.9 487,025 + +1.0 487,026 - -0.6 487,026 - +0.6 487,026 - +0.4 487,026 - +0.1 487,026 - -0.0 487,067 + -0.5 487,067 + -0.6 487,068 - -1.0 487,068 - +0.1
Or see this region's nucleotide sequence