Experiment: Polymyxin B sulfate 4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_02250 and AO353_02255 are separated by 140 nucleotides AO353_02255 and AO353_02260 are separated by 101 nucleotides
AO353_02250: AO353_02250 - chorismate synthase, at 480,972 to 482,063
_02250
AO353_02255: AO353_02255 - alpha/beta hydrolase, at 482,204 to 483,172
_02255
AO353_02260: AO353_02260 - hypothetical protein, at 483,274 to 484,065
_02260
Position (kb) 482
483
484 Strain fitness (log2 ratio) -2
-1
0
1 at 481.493 kb on - strand, within AO353_02250 at 481.493 kb on - strand, within AO353_02250 at 481.720 kb on + strand, within AO353_02250 at 481.733 kb on - strand, within AO353_02250 at 481.738 kb on + strand, within AO353_02250 at 482.051 kb on + strand at 482.109 kb on - strand at 482.109 kb on - strand at 482.122 kb on - strand at 482.122 kb on - strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.123 kb on + strand at 482.124 kb on - strand at 482.124 kb on - strand at 482.124 kb on - strand at 482.140 kb on + strand at 482.140 kb on + strand at 482.140 kb on + strand at 482.140 kb on + strand at 482.141 kb on - strand at 482.141 kb on - strand at 482.141 kb on - strand at 482.141 kb on - strand at 482.141 kb on - strand at 482.218 kb on + strand at 482.218 kb on + strand at 482.219 kb on - strand at 482.219 kb on - strand at 482.219 kb on - strand at 482.243 kb on - strand at 482.243 kb on - strand at 482.280 kb on + strand at 482.280 kb on + strand at 482.280 kb on + strand at 482.280 kb on + strand at 482.280 kb on + strand at 482.280 kb on + strand at 482.280 kb on + strand at 482.281 kb on - strand at 482.281 kb on - strand at 482.414 kb on - strand, within AO353_02255 at 482.561 kb on + strand, within AO353_02255 at 482.561 kb on + strand, within AO353_02255 at 482.561 kb on + strand, within AO353_02255 at 482.561 kb on + strand, within AO353_02255 at 482.561 kb on + strand, within AO353_02255 at 482.561 kb on + strand, within AO353_02255 at 482.562 kb on - strand, within AO353_02255 at 482.562 kb on - strand, within AO353_02255 at 482.562 kb on - strand, within AO353_02255 at 482.612 kb on - strand, within AO353_02255 at 482.725 kb on - strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.876 kb on + strand, within AO353_02255 at 482.877 kb on - strand, within AO353_02255 at 482.877 kb on - strand, within AO353_02255 at 482.877 kb on - strand, within AO353_02255 at 482.877 kb on - strand, within AO353_02255 at 482.877 kb on - strand, within AO353_02255 at 483.092 kb on + strand at 483.092 kb on + strand at 483.093 kb on - strand at 483.093 kb on - strand at 483.093 kb on - strand at 483.093 kb on - strand at 483.148 kb on + strand at 483.148 kb on + strand at 483.170 kb on + strand at 483.170 kb on + strand at 483.170 kb on + strand at 483.170 kb on + strand at 483.170 kb on + strand at 483.171 kb on - strand at 483.171 kb on - strand at 483.182 kb on - strand at 483.645 kb on + strand, within AO353_02260 at 483.645 kb on + strand, within AO353_02260 at 483.645 kb on + strand, within AO353_02260 at 483.646 kb on - strand, within AO353_02260 at 483.646 kb on - strand, within AO353_02260 at 483.646 kb on - strand, within AO353_02260 at 483.673 kb on + strand, within AO353_02260 at 483.673 kb on + strand, within AO353_02260 at 483.674 kb on - strand, within AO353_02260 at 483.674 kb on - strand, within AO353_02260 at 483.775 kb on + strand, within AO353_02260 at 483.776 kb on - strand, within AO353_02260 at 483.776 kb on - strand, within AO353_02260 at 483.815 kb on + strand, within AO353_02260 at 483.816 kb on - strand, within AO353_02260 at 483.816 kb on - strand, within AO353_02260 at 483.964 kb on + strand, within AO353_02260 at 483.964 kb on + strand, within AO353_02260 at 483.965 kb on - strand, within AO353_02260 at 483.965 kb on - strand, within AO353_02260 at 483.986 kb on + strand at 483.986 kb on + strand at 483.986 kb on + strand at 483.987 kb on - strand at 483.987 kb on - strand at 483.987 kb on - strand at 484.054 kb on - strand at 484.054 kb on - strand at 484.064 kb on - strand at 484.167 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Polymyxin B sulfate 4 mg/ml remove 481,493 - AO353_02250 0.48 -1.8 481,493 - AO353_02250 0.48 -2.6 481,720 + AO353_02250 0.68 -1.0 481,733 - AO353_02250 0.70 +0.8 481,738 + AO353_02250 0.70 -1.4 482,051 + -1.0 482,109 - -0.5 482,109 - -0.6 482,122 - -0.0 482,122 - +0.5 482,123 + -0.2 482,123 + +0.3 482,123 + -0.6 482,123 + -0.9 482,123 + -0.4 482,123 + -1.1 482,123 + +0.3 482,123 + +0.6 482,123 + +1.6 482,123 + -1.1 482,124 - +1.4 482,124 - +1.1 482,124 - +0.3 482,140 + -0.6 482,140 + -2.2 482,140 + +1.0 482,140 + -1.0 482,141 - -1.0 482,141 - -1.8 482,141 - +0.1 482,141 - -0.4 482,141 - -0.2 482,218 + -1.0 482,218 + +1.6 482,219 - -1.4 482,219 - -0.4 482,219 - -1.4 482,243 - +0.6 482,243 - +0.8 482,280 + +0.2 482,280 + -1.0 482,280 + -1.4 482,280 + +1.3 482,280 + -2.4 482,280 + -0.7 482,280 + -0.5 482,281 - -2.0 482,281 - +1.3 482,414 - AO353_02255 0.22 -1.4 482,561 + AO353_02255 0.37 +0.8 482,561 + AO353_02255 0.37 -0.3 482,561 + AO353_02255 0.37 -0.6 482,561 + AO353_02255 0.37 -1.2 482,561 + AO353_02255 0.37 -0.5 482,561 + AO353_02255 0.37 +0.1 482,562 - AO353_02255 0.37 -0.8 482,562 - AO353_02255 0.37 -1.0 482,562 - AO353_02255 0.37 +0.0 482,612 - AO353_02255 0.42 -0.2 482,725 - AO353_02255 0.54 -1.2 482,876 + AO353_02255 0.69 -0.2 482,876 + AO353_02255 0.69 -0.6 482,876 + AO353_02255 0.69 -2.0 482,876 + AO353_02255 0.69 -2.6 482,876 + AO353_02255 0.69 -1.4 482,876 + AO353_02255 0.69 -1.6 482,876 + AO353_02255 0.69 -1.1 482,876 + AO353_02255 0.69 -0.7 482,876 + AO353_02255 0.69 -0.1 482,876 + AO353_02255 0.69 -1.3 482,877 - AO353_02255 0.69 -0.0 482,877 - AO353_02255 0.69 -0.5 482,877 - AO353_02255 0.69 -1.3 482,877 - AO353_02255 0.69 -0.6 482,877 - AO353_02255 0.69 +1.2 483,092 + -1.9 483,092 + -2.0 483,093 - -0.9 483,093 - -0.7 483,093 - -0.3 483,093 - -1.4 483,148 + -0.2 483,148 + -0.0 483,170 + +0.1 483,170 + +0.5 483,170 + -0.5 483,170 + +0.1 483,170 + -0.1 483,171 - +0.9 483,171 - -0.6 483,182 - -0.2 483,645 + AO353_02260 0.47 +0.2 483,645 + AO353_02260 0.47 +0.1 483,645 + AO353_02260 0.47 +0.6 483,646 - AO353_02260 0.47 +0.6 483,646 - AO353_02260 0.47 -0.4 483,646 - AO353_02260 0.47 -0.8 483,673 + AO353_02260 0.50 +0.4 483,673 + AO353_02260 0.50 +0.4 483,674 - AO353_02260 0.51 +0.4 483,674 - AO353_02260 0.51 -1.9 483,775 + AO353_02260 0.63 -0.1 483,776 - AO353_02260 0.63 -0.4 483,776 - AO353_02260 0.63 -2.2 483,815 + AO353_02260 0.68 +1.2 483,816 - AO353_02260 0.68 -0.1 483,816 - AO353_02260 0.68 -0.2 483,964 + AO353_02260 0.87 +0.6 483,964 + AO353_02260 0.87 +0.0 483,965 - AO353_02260 0.87 +0.7 483,965 - AO353_02260 0.87 -0.2 483,986 + +1.4 483,986 + -0.4 483,986 + -0.1 483,987 - +0.2 483,987 - -0.5 483,987 - -1.2 484,054 - +0.3 484,054 - +0.4 484,064 - +0.1 484,167 + -1.5
Or see this region's nucleotide sequence