Experiment: MoYLS4 with Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1413 and DvMF_1414 are separated by 102 nucleotides DvMF_1414 and DvMF_1415 are separated by 154 nucleotides DvMF_1415 and DvMF_1416 are separated by 24 nucleotides DvMF_1416 and DvMF_1417 are separated by 51 nucleotides
DvMF_1413: DvMF_1413 - hypothetical protein (RefSeq), at 1,727,449 to 1,728,258
_1413
DvMF_1414: DvMF_1414 - Phosphoglycerate mutase (RefSeq), at 1,728,361 to 1,729,626
_1414
DvMF_1415: DvMF_1415 - hypothetical protein (RefSeq), at 1,729,781 to 1,730,092
_1415
DvMF_1416: DvMF_1416 - hypothetical protein (RefSeq), at 1,730,117 to 1,730,554
_1416
DvMF_1417: DvMF_1417 - heavy metal translocating P-type ATPase (RefSeq), at 1,730,606 to 1,733,521
_1417
Position (kb) 1728
1729
1730 Strain fitness (log2 ratio) -1
0
1 at 1728.458 kb on + strand at 1728.460 kb on - strand at 1728.466 kb on - strand at 1728.977 kb on - strand, within DvMF_1414 at 1729.166 kb on + strand, within DvMF_1414 at 1729.166 kb on + strand, within DvMF_1414 at 1729.226 kb on + strand, within DvMF_1414 at 1729.285 kb on + strand, within DvMF_1414 at 1729.351 kb on + strand, within DvMF_1414 at 1729.990 kb on + strand, within DvMF_1415 at 1730.094 kb on - strand at 1730.184 kb on + strand, within DvMF_1416 at 1730.499 kb on - strand, within DvMF_1416 at 1730.514 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Fusidic 0.03125 mg/ml remove 1,728,458 + +0.1 1,728,460 - -0.0 1,728,466 - +0.0 1,728,977 - DvMF_1414 0.49 +1.1 1,729,166 + DvMF_1414 0.64 +0.3 1,729,166 + DvMF_1414 0.64 -0.1 1,729,226 + DvMF_1414 0.68 +0.5 1,729,285 + DvMF_1414 0.73 +0.3 1,729,351 + DvMF_1414 0.78 +1.6 1,729,990 + DvMF_1415 0.67 -1.4 1,730,094 - -0.5 1,730,184 + DvMF_1416 0.15 -0.7 1,730,499 - DvMF_1416 0.87 -0.5 1,730,514 + -0.7
Or see this region's nucleotide sequence