Experiment: MoYLS4 with Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0942 and DvMF_0943 are separated by 331 nucleotides DvMF_0943 and DvMF_0944 are separated by 408 nucleotides DvMF_0944 and DvMF_0945 are separated by 612 nucleotides
DvMF_0942: DvMF_0942 - N-(5'phosphoribosyl)anthranilate isomerase (PRAI) (RefSeq), at 1,142,845 to 1,143,618
_0942
DvMF_0943: DvMF_0943 - hypothetical protein (RefSeq), at 1,143,950 to 1,144,045
_0943
DvMF_0944: DvMF_0944 - protein of unknown function DUF6 transmembrane (RefSeq), at 1,144,454 to 1,145,299
_0944
DvMF_0945: DvMF_0945 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 1,145,912 to 1,146,946
_0945
Position (kb)
1144
1145
1146 Strain fitness (log2 ratio)
-1
0
1 at 1143.506 kb on + strand, within DvMF_0942 at 1143.601 kb on + strand at 1143.757 kb on - strand at 1143.782 kb on + strand at 1143.782 kb on + strand at 1143.790 kb on - strand at 1143.790 kb on - strand at 1143.790 kb on - strand at 1143.790 kb on - strand at 1143.828 kb on + strand at 1144.527 kb on - strand at 1144.662 kb on - strand, within DvMF_0944 at 1144.880 kb on - strand, within DvMF_0944 at 1144.975 kb on + strand, within DvMF_0944 at 1144.983 kb on - strand, within DvMF_0944 at 1145.030 kb on - strand, within DvMF_0944 at 1145.053 kb on + strand, within DvMF_0944 at 1145.236 kb on - strand at 1145.274 kb on + strand at 1145.279 kb on + strand at 1145.383 kb on + strand at 1145.556 kb on + strand at 1145.647 kb on - strand at 1146.000 kb on + strand at 1146.024 kb on - strand, within DvMF_0945 at 1146.130 kb on - strand, within DvMF_0945
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Fusidic 0.03125 mg/ml remove 1,143,506 + DvMF_0942 0.85 -0.6 1,143,601 + -0.7 1,143,757 - -0.8 1,143,782 + -1.4 1,143,782 + -0.1 1,143,790 - -0.6 1,143,790 - -0.3 1,143,790 - -1.0 1,143,790 - -0.4 1,143,828 + -1.0 1,144,527 - -0.9 1,144,662 - DvMF_0944 0.25 -0.7 1,144,880 - DvMF_0944 0.50 +0.5 1,144,975 + DvMF_0944 0.62 -0.2 1,144,983 - DvMF_0944 0.63 -1.0 1,145,030 - DvMF_0944 0.68 -0.3 1,145,053 + DvMF_0944 0.71 -0.2 1,145,236 - -0.6 1,145,274 + -0.7 1,145,279 + +0.2 1,145,383 + -0.4 1,145,556 + -0.1 1,145,647 - +0.1 1,146,000 + -1.4 1,146,024 - DvMF_0945 0.11 -0.9 1,146,130 - DvMF_0945 0.21 -0.4
Or see this region's nucleotide sequence