Experiment: MoYLS4 with Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ileS and DvMF_0267 are separated by 365 nucleotides DvMF_0267 and DvMF_0268 are separated by 147 nucleotides DvMF_0268 and DvMF_0269 are separated by 62 nucleotides
DvMF_0266: ileS - isoleucyl-tRNA synthetase (RefSeq), at 302,686 to 305,502
ileS
DvMF_0267: DvMF_0267 - lipolytic protein G-D-S-L family (RefSeq), at 305,868 to 306,515
_0267
DvMF_0268: DvMF_0268 - hydrogenase assembly chaperone hypC/hupF (RefSeq), at 306,663 to 306,914
_0268
DvMF_0269: DvMF_0269 - hydrogenase expression/formation protein (RefSeq), at 306,977 to 307,483
_0269
Position (kb) 305
306
307 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 305.981 kb on + strand, within DvMF_0267 at 306.135 kb on - strand, within DvMF_0267 at 306.466 kb on - strand at 306.507 kb on - strand at 306.653 kb on - strand at 306.758 kb on - strand, within DvMF_0268 at 307.252 kb on + strand, within DvMF_0269 at 307.318 kb on - strand, within DvMF_0269 at 307.318 kb on - strand, within DvMF_0269 at 307.380 kb on + strand, within DvMF_0269 at 307.431 kb on + strand, within DvMF_0269 at 307.499 kb on - strand at 307.502 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Fusidic 0.03125 mg/ml remove 305,981 + DvMF_0267 0.17 +0.1 306,135 - DvMF_0267 0.41 +0.5 306,466 - -0.6 306,507 - +0.1 306,653 - -0.9 306,758 - DvMF_0268 0.38 +1.9 307,252 + DvMF_0269 0.54 -1.2 307,318 - DvMF_0269 0.67 -0.7 307,318 - DvMF_0269 0.67 +0.4 307,380 + DvMF_0269 0.79 +0.1 307,431 + DvMF_0269 0.90 +0.1 307,499 - -4.8 307,502 - -1.4
Or see this region's nucleotide sequence