Experiment: MoYLS4 with Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0164 and DvMF_0165 are separated by 121 nucleotides DvMF_0165 and DvMF_0166 are separated by 11 nucleotides DvMF_0166 and DvMF_0167 are separated by 135 nucleotides
DvMF_0164: DvMF_0164 - hypothetical protein (RefSeq), at 178,837 to 179,658
_0164
DvMF_0165: DvMF_0165 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 179,780 to 180,337
_0165
DvMF_0166: DvMF_0166 - Formate dehydrogenase (RefSeq), at 180,349 to 182,484
_0166
DvMF_0167: DvMF_0167 - RNA polymerase, sigma-24 subunit, ECF subfamily (RefSeq), at 182,620 to 184,020
_0167
Position (kb) 180
181
182
183 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 179.499 kb on + strand, within DvMF_0164 at 179.523 kb on + strand, within DvMF_0164 at 180.066 kb on + strand, within DvMF_0165 at 180.110 kb on - strand, within DvMF_0165 at 180.407 kb on - strand at 180.502 kb on + strand at 180.783 kb on + strand, within DvMF_0166 at 180.783 kb on + strand, within DvMF_0166 at 180.970 kb on + strand, within DvMF_0166 at 181.079 kb on + strand, within DvMF_0166 at 181.167 kb on + strand, within DvMF_0166 at 181.175 kb on - strand, within DvMF_0166 at 181.251 kb on + strand, within DvMF_0166 at 181.299 kb on + strand, within DvMF_0166 at 181.411 kb on + strand, within DvMF_0166 at 181.419 kb on - strand, within DvMF_0166 at 181.496 kb on + strand, within DvMF_0166 at 181.697 kb on + strand, within DvMF_0166 at 181.705 kb on - strand, within DvMF_0166 at 181.850 kb on - strand, within DvMF_0166 at 181.850 kb on - strand, within DvMF_0166 at 181.871 kb on + strand, within DvMF_0166 at 181.997 kb on + strand, within DvMF_0166 at 181.999 kb on + strand, within DvMF_0166 at 182.054 kb on - strand, within DvMF_0166 at 182.427 kb on - strand at 182.438 kb on + strand at 182.438 kb on + strand at 182.546 kb on + strand at 182.554 kb on - strand at 182.818 kb on + strand, within DvMF_0167 at 182.913 kb on - strand, within DvMF_0167 at 182.972 kb on - strand, within DvMF_0167 at 182.982 kb on - strand, within DvMF_0167 at 183.014 kb on - strand, within DvMF_0167 at 183.032 kb on - strand, within DvMF_0167 at 183.214 kb on + strand, within DvMF_0167 at 183.261 kb on - strand, within DvMF_0167 at 183.437 kb on - strand, within DvMF_0167
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Fusidic 0.03125 mg/ml remove 179,499 + DvMF_0164 0.81 -0.3 179,523 + DvMF_0164 0.83 -0.3 180,066 + DvMF_0165 0.51 -1.7 180,110 - DvMF_0165 0.59 -3.1 180,407 - +0.3 180,502 + -0.1 180,783 + DvMF_0166 0.20 -0.1 180,783 + DvMF_0166 0.20 -1.7 180,970 + DvMF_0166 0.29 +0.6 181,079 + DvMF_0166 0.34 -0.5 181,167 + DvMF_0166 0.38 +0.7 181,175 - DvMF_0166 0.39 -1.1 181,251 + DvMF_0166 0.42 -0.6 181,299 + DvMF_0166 0.44 -1.6 181,411 + DvMF_0166 0.50 -1.1 181,419 - DvMF_0166 0.50 +2.4 181,496 + DvMF_0166 0.54 -0.4 181,697 + DvMF_0166 0.63 -0.3 181,705 - DvMF_0166 0.63 -0.9 181,850 - DvMF_0166 0.70 -0.4 181,850 - DvMF_0166 0.70 -0.3 181,871 + DvMF_0166 0.71 -0.9 181,997 + DvMF_0166 0.77 -0.9 181,999 + DvMF_0166 0.77 -0.4 182,054 - DvMF_0166 0.80 -1.3 182,427 - -0.9 182,438 + -0.4 182,438 + -0.5 182,546 + -0.5 182,554 - -0.7 182,818 + DvMF_0167 0.14 +0.0 182,913 - DvMF_0167 0.21 -0.5 182,972 - DvMF_0167 0.25 +0.1 182,982 - DvMF_0167 0.26 -1.0 183,014 - DvMF_0167 0.28 -0.7 183,032 - DvMF_0167 0.29 -0.7 183,214 + DvMF_0167 0.42 -0.2 183,261 - DvMF_0167 0.46 -1.2 183,437 - DvMF_0167 0.58 -1.3
Or see this region's nucleotide sequence