Experiment: MoYLS4 with Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0029 and DvMF_0030 are separated by 756 nucleotides DvMF_0030 and DvMF_0031 are separated by 298 nucleotides DvMF_0031 and DvMF_0032 are separated by 276 nucleotides
DvMF_0029: DvMF_0029 - hypothetical protein (RefSeq), at 37,215 to 37,841
_0029
DvMF_0030: DvMF_0030 - hypothetical protein (RefSeq), at 38,598 to 38,939
_0030
DvMF_0031: DvMF_0031 - hypothetical protein (RefSeq), at 39,238 to 39,513
_0031
DvMF_0032: DvMF_0032 - diguanylate cyclase (RefSeq), at 39,790 to 41,526
_0032
Position (kb) 38
39 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 37.843 kb on + strand at 37.977 kb on - strand at 37.997 kb on + strand at 38.069 kb on + strand at 38.140 kb on - strand at 38.251 kb on + strand at 38.371 kb on + strand at 38.400 kb on + strand at 38.469 kb on - strand at 38.482 kb on - strand at 38.558 kb on - strand at 38.569 kb on - strand at 38.663 kb on + strand, within DvMF_0030 at 38.703 kb on + strand, within DvMF_0030 at 38.788 kb on - strand, within DvMF_0030 at 38.913 kb on - strand at 38.931 kb on + strand at 38.996 kb on - strand at 39.227 kb on - strand at 39.295 kb on + strand, within DvMF_0031 at 39.303 kb on - strand, within DvMF_0031 at 39.765 kb on + strand at 39.774 kb on + strand at 39.798 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Fusidic 0.03125 mg/ml remove 37,843 + -0.6 37,977 - -0.9 37,997 + +2.0 38,069 + -2.0 38,140 - -1.0 38,251 + -0.7 38,371 + -0.9 38,400 + -0.3 38,469 - +0.2 38,482 - -0.3 38,558 - +0.3 38,569 - +0.1 38,663 + DvMF_0030 0.19 -0.3 38,703 + DvMF_0030 0.31 -3.8 38,788 - DvMF_0030 0.56 -2.1 38,913 - -0.5 38,931 + -0.2 38,996 - -1.3 39,227 - -0.5 39,295 + DvMF_0031 0.21 -0.6 39,303 - DvMF_0031 0.24 -0.4 39,765 + -0.4 39,774 + -0.6 39,798 + -0.3
Or see this region's nucleotide sequence