Experiment: MoYLS4 with Fusidic 0.03125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0022 and DvMF_0023 are separated by 58 nucleotides DvMF_0023 and DvMF_0024 are separated by 81 nucleotides DvMF_0024 and DvMF_0025 are separated by 44 nucleotides DvMF_0025 and DvMF_0026 are separated by 235 nucleotides
DvMF_0022: DvMF_0022 - metal dependent phosphohydrolase (RefSeq), at 28,080 to 29,165
_0022
DvMF_0023: DvMF_0023 - dTMP kinase (RefSeq), at 29,224 to 29,871
_0023
DvMF_0024: DvMF_0024 - histone deacetylase superfamily (RefSeq), at 29,953 to 31,272
_0024
DvMF_0025: DvMF_0025 - protein of unknown function DUF1121 (RefSeq), at 31,317 to 32,003
_0025
DvMF_0026: DvMF_0026 - succinyl-CoA synthetase, alpha subunit (RefSeq), at 32,239 to 34,368
_0026
Position (kb) 29
30
31
32 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 29.002 kb on - strand, within DvMF_0022 at 29.928 kb on + strand at 30.401 kb on + strand, within DvMF_0024 at 30.587 kb on - strand, within DvMF_0024 at 30.616 kb on - strand, within DvMF_0024 at 30.725 kb on - strand, within DvMF_0024 at 30.900 kb on - strand, within DvMF_0024 at 31.335 kb on - strand at 31.678 kb on + strand, within DvMF_0025 at 31.793 kb on - strand, within DvMF_0025 at 31.803 kb on - strand, within DvMF_0025 at 31.865 kb on - strand, within DvMF_0025 at 32.154 kb on - strand at 32.164 kb on - strand at 32.165 kb on - strand at 32.175 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Fusidic 0.03125 mg/ml remove 29,002 - DvMF_0022 0.85 +0.1 29,928 + -2.0 30,401 + DvMF_0024 0.34 -0.4 30,587 - DvMF_0024 0.48 +0.2 30,616 - DvMF_0024 0.50 +0.7 30,725 - DvMF_0024 0.58 -2.4 30,900 - DvMF_0024 0.72 +0.2 31,335 - -0.4 31,678 + DvMF_0025 0.53 -1.0 31,793 - DvMF_0025 0.69 -2.1 31,803 - DvMF_0025 0.71 -0.9 31,865 - DvMF_0025 0.80 +1.4 32,154 - -1.0 32,164 - -0.9 32,165 - +0.1 32,175 - -2.9
Or see this region's nucleotide sequence