Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3021 and acpS are separated by 170 nucleotides acpS and Shewana3_3023 overlap by 1 nucleotides Shewana3_3023 and Shewana3_3024 are separated by 89 nucleotides Shewana3_3024 and era are separated by 49 nucleotides
Shewana3_3021: Shewana3_3021 - hypothetical protein (RefSeq), at 3,627,744 to 3,628,280
_3021
Shewana3_3022: acpS - 4'-phosphopantetheinyl transferase (RefSeq), at 3,628,451 to 3,628,834
acpS
Shewana3_3023: Shewana3_3023 - pyridoxine 5'-phosphate synthase (RefSeq), at 3,628,834 to 3,629,571
_3023
Shewana3_3024: Shewana3_3024 - DNA repair protein RecO (RefSeq), at 3,629,661 to 3,630,362
_3024
Shewana3_3025: era - GTP-binding protein Era (RefSeq), at 3,630,412 to 3,631,431
era
Position (kb)
3628
3629
3630 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3628.073 kb on - strand, within Shewana3_3021 at 3628.544 kb on - strand, within acpS at 3628.589 kb on - strand, within acpS at 3628.621 kb on - strand, within acpS at 3628.646 kb on + strand, within acpS at 3628.778 kb on - strand, within acpS at 3629.629 kb on - strand at 3629.629 kb on - strand at 3629.840 kb on + strand, within Shewana3_3024 at 3629.975 kb on + strand, within Shewana3_3024 at 3629.983 kb on - strand, within Shewana3_3024 at 3629.983 kb on - strand, within Shewana3_3024 at 3629.990 kb on + strand, within Shewana3_3024 at 3630.066 kb on - strand, within Shewana3_3024 at 3630.187 kb on - strand, within Shewana3_3024 at 3630.328 kb on - strand at 3630.409 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,628,073 - Shewana3_3021 0.61 -0.7 3,628,544 - acpS Shewana3_3022 0.24 -1.2 3,628,589 - acpS Shewana3_3022 0.36 -1.0 3,628,621 - acpS Shewana3_3022 0.44 +1.2 3,628,646 + acpS Shewana3_3022 0.51 +0.2 3,628,778 - acpS Shewana3_3022 0.85 +0.9 3,629,629 - +0.9 3,629,629 - -1.1 3,629,840 + Shewana3_3024 0.25 +0.2 3,629,975 + Shewana3_3024 0.45 -1.3 3,629,983 - Shewana3_3024 0.46 -2.3 3,629,983 - Shewana3_3024 0.46 -1.7 3,629,990 + Shewana3_3024 0.47 -1.6 3,630,066 - Shewana3_3024 0.58 -4.1 3,630,187 - Shewana3_3024 0.75 -2.0 3,630,328 - -1.2 3,630,409 + -1.5
Or see this region's nucleotide sequence