Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaE and rnhB are separated by 117 nucleotides rnhB and lpxB overlap by 1 nucleotides lpxB and Shewana3_2803 are separated by 116 nucleotides Shewana3_2803 and fabZ overlap by 4 nucleotides
Shewana3_2800: dnaE - DNA polymerase III subunit alpha (RefSeq), at 3,344,158 to 3,347,634
dnaE
Shewana3_2801: rnhB - ribonuclease HII (RefSeq), at 3,347,752 to 3,348,381
rnhB
Shewana3_2802: lpxB - lipid-A-disaccharide synthase (RefSeq), at 3,348,381 to 3,349,538
lpxB
Shewana3_2803: Shewana3_2803 - UDP-N-acetylglucosamine acyltransferase (RefSeq), at 3,349,655 to 3,350,425
_2803
Shewana3_2804: fabZ - (3R)-hydroxymyristoyl-ACP dehydratase (RefSeq), at 3,350,422 to 3,350,874
fabZ
Position (kb) 3348
3349
3350 Strain fitness (log2 ratio) -1
0
1
2
3 at 3347.757 kb on - strand at 3347.757 kb on - strand at 3347.843 kb on + strand, within rnhB at 3347.852 kb on + strand, within rnhB at 3347.860 kb on - strand, within rnhB at 3347.860 kb on - strand, within rnhB at 3348.269 kb on + strand, within rnhB at 3348.330 kb on - strand at 3348.426 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,347,757 - -0.0 3,347,757 - -0.2 3,347,843 + rnhB Shewana3_2801 0.14 +0.4 3,347,852 + rnhB Shewana3_2801 0.16 -0.6 3,347,860 - rnhB Shewana3_2801 0.17 +3.0 3,347,860 - rnhB Shewana3_2801 0.17 +0.5 3,348,269 + rnhB Shewana3_2801 0.82 +2.4 3,348,330 - +0.8 3,348,426 - +1.4
Or see this region's nucleotide sequence