Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2043 and infC are separated by 114 nucleotides infC and rpmI are separated by 85 nucleotides rpmI and rplT are separated by 16 nucleotides rplT and Shewana3_2047 are separated by 193 nucleotides
Shewana3_2043: Shewana3_2043 - threonyl-tRNA synthetase (RefSeq), at 2,451,269 to 2,453,197
_2043
Shewana3_2044: infC - translation initiation factor IF-3 (RefSeq), at 2,453,312 to 2,453,743
infC
Shewana3_2045: rpmI - 50S ribosomal protein L35 (RefSeq), at 2,453,829 to 2,454,023
rpmI
Shewana3_2046: rplT - 50S ribosomal protein L20 (RefSeq), at 2,454,040 to 2,454,396
rplT
Shewana3_2047: Shewana3_2047 - thioredoxin reductase (RefSeq), at 2,454,590 to 2,455,543
_2047
Position (kb) 2454
2455 Strain fitness (log2 ratio) -1
0
1
2
3 at 2454.614 kb on - strand at 2455.093 kb on - strand, within Shewana3_2047 at 2455.175 kb on + strand at 2455.175 kb on + strand, within Shewana3_2047 at 2455.183 kb on - strand, within Shewana3_2047 at 2455.183 kb on - strand, within Shewana3_2047 at 2455.219 kb on + strand, within Shewana3_2047
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,454,614 - +1.4 2,455,093 - Shewana3_2047 0.53 +1.6 2,455,175 + +2.8 2,455,175 + Shewana3_2047 0.61 +1.8 2,455,183 - Shewana3_2047 0.62 +1.8 2,455,183 - Shewana3_2047 0.62 +2.0 2,455,219 + Shewana3_2047 0.66 +0.4
Or see this region's nucleotide sequence